Construct: ORF ccsbBroad304_05599
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004773.1_s304c1, BRDN0000401295
- Derived from:
- ccsbBroadEn_05599
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MUSTN1 (389125)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_05599
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 389125 | MUSTN1 | musculoskeletal, embryonic ... | NM_205853.4 | 99.5% | 98.7% | 60C>G |
| 2 | human | 100526772 | STIMATE-MUSTN1 | STIMATE-MUSTN1 readthrough | NM_001198974.3 | 21.4% | 20.9% | (many diffs) |
| 3 | mouse | 66175 | Mustn1 | musculoskeletal, embryonic ... | NM_181390.3 | 85.3% | 85.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 315
- ORF length:
- 246
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat gtcccaggct ggtgctcagg aagcccctat caagaagaag cgcccccctg 121 tgaaggagga ggacctgaag ggggcccgag gaaacctgac caagaaccag gaaatcaagt 181 ccaagaccta ccaggtcatg cgagagtgtg agcaagctgg ctcggccgcc ccgtcggtgt 241 tcagccgcac ccgcacaggt accgagactg tctttgagaa gcccaaagcc ggacccacca 301 agagtgtctt cggcttgcca actttcttgt acaaagtggt tggtaagcct atccctaacc 361 ctctcctcgg tctcgattct acgtagtaat gagctagcgc taaccggtgg cgcgttaagt 421 cgacaatcaa cctctggatt a