Transcript: Human NM_001198974.3

Homo sapiens STIMATE-MUSTN1 readthrough (STIMATE-MUSTN1), mRNA.

Source:
NCBI, updated 2019-06-02
Taxon:
Homo sapiens (human)
Gene:
STIMATE-MUSTN1 (100526772)
Length:
1420
CDS:
99..1217

Additional Resources:

NCBI RefSeq record:
NM_001198974.3
NBCI Gene record:
STIMATE-MUSTN1 (100526772)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001198974.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000418871 GAATGCTGTTCATCCACTTTG pLKO_005 337 CDS 100% 10.800 5.400 Y STIMATE n/a
2 TRCN0000370398 ACCTGACCAAGAACCAGGAAA pLKO_005 1054 CDS 100% 4.950 2.475 Y MUSTN1 n/a
3 TRCN0000377570 CCGAGGAAACCTGACCAAGAA pLKO_005 1046 CDS 100% 4.950 2.475 Y MUSTN1 n/a
4 TRCN0000129193 CCTTCTTTGTCAACGCTTTGA pLKO.1 703 CDS 100% 4.950 2.475 Y STIMATE n/a
5 TRCN0000149795 CGCTCTTTACATCGTGATCAT pLKO.1 563 CDS 100% 4.950 2.475 Y STIMATE n/a
6 TRCN0000444207 CGTCCGTGGAGGATATGGTTT pLKO_005 291 CDS 100% 4.950 2.475 Y STIMATE n/a
7 TRCN0000426443 GACGAAAGCTAAGCTAGAAGA pLKO_005 764 CDS 100% 4.950 2.475 Y STIMATE n/a
8 TRCN0000244703 GAGCCTTTCCCAGGAACTCTT pLKO_005 1262 3UTR 100% 4.950 2.475 Y MUSTN1 n/a
9 TRCN0000429157 GCGCTCTTTACATCGTGATCA pLKO_005 562 CDS 100% 4.950 2.475 Y STIMATE n/a
10 TRCN0000127828 GTCTGAGTCTGAGATCCTGAT pLKO.1 854 CDS 100% 4.050 2.025 Y STIMATE n/a
11 TRCN0000127960 CGCTTCAGAGAACCAAAGCAT pLKO.1 264 CDS 100% 3.000 1.500 Y STIMATE n/a
12 TRCN0000370343 ATCAAGTCCAAGACCTACCAG pLKO_005 1074 CDS 100% 2.640 1.320 Y MUSTN1 n/a
13 TRCN0000127756 GCACGTTAATGCTCAAACGCT pLKO.1 247 CDS 100% 0.750 0.375 Y STIMATE n/a
14 TRCN0000200433 GAGTCTGAGATCCTGATCTCA pLKO.1 858 CDS 100% 0.300 0.150 Y Tmem110 n/a
15 TRCN0000244705 TACCAGGTCATGCGAGAGTGT pLKO_005 1089 CDS 100% 0.000 0.000 Y MUSTN1 n/a
16 TRCN0000244704 TACCGAGACTGTCTTTGAGAA pLKO_005 1160 CDS 100% 0.000 0.000 Y MUSTN1 n/a
17 TRCN0000250931 CACTGTACCTCATCAACTTTC pLKO_005 400 CDS 100% 10.800 5.400 Y Tmem110 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001198974.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_05547 pDONR223 100% 78.8% 78.7% None 881_1116delinsTA n/a
2 ccsbBroad304_05547 pLX_304 0% 78.8% 78.7% V5 881_1116delinsTA n/a
3 TRCN0000472940 TCTGCCAGAAGTTCTTTTGAGCCA pLX_317 49.1% 78.8% 78.7% V5 881_1116delinsTA n/a
4 ccsbBroadEn_05599 pDONR223 100% 21.4% 20.9% None (many diffs) n/a
5 ccsbBroad304_05599 pLX_304 0% 21.4% 20.9% V5 (many diffs) n/a
6 TRCN0000465881 TGAGTTATTATACCGCAGATCAAG pLX_317 100% 21.4% 20.9% V5 (many diffs) n/a
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