Construct: ORF ccsbBroad304_05647
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008517.1_s304c1, BRDN0000407333
- Derived from:
- ccsbBroadEn_05647
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TOMM5 (401505)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_05647
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 401505 | TOMM5 | translocase of outer mitoch... | NM_001001790.3 | 100% | 100% | |
| 2 | human | 401505 | TOMM5 | translocase of outer mitoch... | NM_001134485.1 | 60% | 58.8% | 122_223del |
| 3 | human | 401505 | TOMM5 | translocase of outer mitoch... | NM_001134484.1 | 50.3% | 40.4% | (many diffs) |
| 4 | human | 107986372 | LOC107986372 | mitochondrial import recept... | XR_002956250.1 | 23.3% | (many diffs) | |
| 5 | human | 107986373 | LOC107986373 | mitochondrial import recept... | XR_002956251.1 | 23.3% | (many diffs) | |
| 6 | mouse | 68512 | Tomm5 | translocase of outer mitoch... | NM_001099675.1 | 90.1% | 96% | (many diffs) |
| 7 | mouse | 68512 | Tomm5 | translocase of outer mitoch... | NM_001134646.1 | 69.4% | 65.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 219
- ORF length:
- 153
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgtt ccggattgag ggcctcgcgc cgaagctgga cccggaggag atgaaacgga 121 agatgcgcga ggatgtgatc tcctccatac ggaactttct catctacgtg gccctcctgc 181 gagtcactcc atttatctta aagaaattgg acagcatatg cccaactttc ttgtacaaag 241 tggttggtaa gcctatccct aaccctctcc tcggtctcga ttctacgtag taatgagcta 301 gcgctaaccg gtggcgcgtt aagtcgacaa tcaacctctg gatta