Construct: ORF ccsbBroad304_05678
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010129.1_s304c1, BRDN0000404681
- Derived from:
- ccsbBroadEn_05678
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TYW1B (441250)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_05678
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 441250 | TYW1B | tRNA-yW synthesizing protei... | NM_001145440.3 | 99.9% | 99.8% | 1532T>C |
| 2 | human | 55253 | TYW1 | tRNA-yW synthesizing protei... | NM_018264.4 | 89.6% | 87.4% | (many diffs) |
| 3 | human | 55253 | TYW1 | tRNA-yW synthesizing protei... | XM_017012392.2 | 81.6% | 81% | (many diffs) |
| 4 | human | 55253 | TYW1 | tRNA-yW synthesizing protei... | XM_011516372.3 | 81% | 78.6% | (many diffs) |
| 5 | human | 55253 | TYW1 | tRNA-yW synthesizing protei... | XR_002956462.1 | 61.7% | (many diffs) | |
| 6 | human | 55253 | TYW1 | tRNA-yW synthesizing protei... | NR_134540.1 | 59.3% | (many diffs) | |
| 7 | human | 55253 | TYW1 | tRNA-yW synthesizing protei... | XR_001744833.2 | 57.2% | (many diffs) | |
| 8 | human | 55253 | TYW1 | tRNA-yW synthesizing protei... | XR_001744834.2 | 57.1% | (many diffs) | |
| 9 | human | 55253 | TYW1 | tRNA-yW synthesizing protei... | XR_001744831.2 | 56.4% | (many diffs) | |
| 10 | human | 55253 | TYW1 | tRNA-yW synthesizing protei... | XR_001744832.2 | 56.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2070
- ORF length:
- 2004
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgga tccttctgcg gatacatggg acctctcctc acctttaata tcattatgga 121 taaacaggtt ttacatttat ctgggctttg ctgttagcat tagcctttgg atttgtgtcc 181 agattgtcat cgagatgcag ggatttgcaa cagttcttgc tgaagcagtt acgtccctgg 241 atctgcctgt ggccattatt aatctaaaag aatatgatcc agatgatcat ctgatagaag 301 aggtgactag taaaaatgtc tgtgtcttcc tggttgcgac atacactgac ggcctaccaa 361 ccgaaagtgc agagtggttc tgcaaatggt tagaggaagc atccattgat tttcgatttg 421 gcaaaactta cctgaagggt atgagagatg cggtatttgg cctgggaaat tctgcctatg 481 ctagccactt caacaaggtt ggcaaaaatg ttgacaagtg gctctggatg cttggcgtgc 541 atcgtgtgat gagtcgaggg gagggcgact gcgacgtggt taaaagcaag cacggcagca 601 ttgaggccaa cttcagagca tggaagacca agttcatctc ccagctgcag gcacttcaga 661 aaggggagag aaagaagtcc tgtggcggcc actgcaagaa aggcaaatgt gaatctcacc 721 aacatggctc agaggagagg gaggaaggat ctcaagagca ggacgaattg catcacagag 781 acaccaagga ggaagaaccc ttcgagagct ccagtgaaga agagtttggt ggtgaggacc 841 atcagagcct aaattccatt gttgatgttg aagatttggg caaaattatg gatcacgtga 901 agaaagaaaa gagagaaaag gaacagcagg aagagaagtc tggtttgttc aggaacatgg 961 ggaggaatga agatggtgaa agaagagcta tgataactcc tgctctccga gaagccctta 1021 ctaaacaagt cgatgctccg agggagagga gcttgttaca aacacacatt ctatggaatg 1081 agagccatcg ctgcatggaa accaccccga gcttggcgtg tgctaataaa tgtgtcttct 1141 gttggtggca ccacaacaac cctgtgggca ctgaatggtt gtggaagatg gaccagcctg 1201 aaatgatctt gaaggaagcc attgaaaacc atcagaacat gattaagcag tttaaaggag 1261 taccgggcgt caaagcagaa cgctttgaag aaggaatgac ggtaaagcac tgtgcattgt 1321 ccctcgtggg agaaccaata atgtacccag agatcaacag gtttttgaag ctactccacc 1381 agtgtaaaat ctccagcttc ctggtcacaa atgcacaatt tcctgcggaa atcaggaacc 1441 tcgagccagt tactcagctg tatgtcagtg tggatgccag taccaaagac agcctgaaga 1501 aaatcgaccg cccactcttc aaggatttct ggcagcaatt ccttgacagt ttaaaagcct 1561 tggcagtcaa gcaacaacga actgtctaca gactgacgct cgtgaaagca tggaacgtgg 1621 acgagctcca ggcctacgcg cagctcgtgt ccctggggaa tcctgacttc atcgaagtga 1681 agggcgttac ctactgcaga gaaagttcag caagcagtct taccatggcc catgtgccct 1741 ggcatgagga agtggtacag tttgtccgcg agctggtgga tctgatcccc gaatatgaaa 1801 ttgcatgtga acacgaacac tctaattgcc tcctgatagc acacagaaag tttaaaattg 1861 gtggtgaatg gtggacatgg atcgattata accgcttcca ggagctcatc caggaatatg 1921 aagatagtgg tggatcaaaa acgttcagcg caaaggatta tatggccaga actcctcact 1981 gggcattatt tggtgccaat gaaagaagct ttgatcccaa ggacacaaga catcagagaa 2041 agaacaaatc aaaggctatt tctggatgtt gcccaacttt cttgtacaaa gtggttggta 2101 agcctatccc taaccctctc ctcggtctcg attctacgta gtaatgagct agcgctaacc 2161 ggtggcgcgt taagtcgaca atcaacctct ggatta