Construct: ORF ccsbBroad304_05772

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF002445.1_s304c1, BRDN0000462403
Derived from:
ccsbBroadEn_05772
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
ADCY2 (108)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_05772
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 108 ADCY2 adenylate cyclase 2 NM_020546.3 99.9% 99.9% 439G>T;1878C>T;2661C>T
2 human 108 ADCY2 adenylate cyclase 2 XM_011513942.2 95.6% 95.6% (many diffs)
3 human 108 ADCY2 adenylate cyclase 2 XR_427657.2 82.2% (many diffs)
4 human 108 ADCY2 adenylate cyclase 2 XR_001741974.2 80.5% (many diffs)
5 human 108 ADCY2 adenylate cyclase 2 XR_001741973.1 70.2% (many diffs)
6 mouse 210044 Adcy2 adenylate cyclase 2 NM_153534.2 88.1% 95.1% (many diffs)
7 mouse 210044 Adcy2 adenylate cyclase 2 XR_001780768.2 67.5% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
3342
ORF length:
3273
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcaccat gtggcaggag gcgatgcggc gccgccgcta cctgcgggac cgctccgagg
121aggcggcggg cggcggagac gggctgccgc ggtcccggga ctggctctac gagtcctact
181actgcatgag ccagcagcac ccgctcatcg tcttcctgct gctcatcgtc atgggctcct
241gcctcgccct gctcgccgtc ttcttcgcgc tcgggctgga agttgaagac catgtggcgt
301ttctaataac agttccaact gccctggcga ttttctttgc gatatttatc ctggtctgca
361tcgagtctgt gtttaagaag ctgctgcgcc tcttctcgtt ggtgatatgg atatgccttg
421ttgccatggg atacctgttc atgtgttttg gaggcaccgt ctctccctgg gaccaggtat
481cgttcttcct cttcatcatc ttcgtgttgt acaccatgct gcccttcaac atgcgagacg
541ccatcattgc cagcgtcctc acctcctcct cccacaccat cgtgcttagc gtctgcctgt
601ctgcaacacc gggaggcaag gagcacctgg tctggcagat cctggccaat gtgatcattt
661tcatctgtgg gaacctggcg ggagcctacc ataagcacct catggaactc gctcttcagc
721aaacatatca ggacacctgt aattgcatca agtcgcggat caagttggaa tttgaaaaac
781gtcaacagga gcggcttctg ctctccctgc tgccggccca catcgccatg gagatgaaag
841cggagatcat ccagaggctg cagggcccca aggcgggcca gatggagaac acaaataact
901tccacaacct gtatgtgaag cggcatacaa acgtgagcat cttatacgct gacatcgttg
961gctttacccg gctggcaagt gactgctccc cgggagaact agtccacatg ctgaatgagc
1021tctttggaaa gtttgatcaa attgcaaagg agaatgaatg catgagaatt aaaattttag
1081gagactgcta ctactgtgta tctggactcc ctatatctct ccctaaccat gccaagaact
1141gtgtgaaaat ggggctggac atgtgtgaag ccataaagaa agtgagggat gctactggag
1201ttgatatcaa catgcgcgtg ggcgtgcatt ctgggaatgt cctgtgtggc gtgattggtc
1261tgcagaagtg gcaatatgat gtgtggtcac atgatgtgac cttggccaac cacatggaag
1321ctggaggggt ccctggacgt gttcacattt cttctgtcac cctggagcac ttgaatggcg
1381cttataaagt ggaggaggga gatggtgaca ttagggaccc atatttaaaa cagcacctgg
1441tgaaaaccta ctttgtgatc aaccccaagg gagaacgacg gagcccccag catctcttca
1501gacctcgcca cacccttgat ggagccaaaa tgagggcctc ggtccgcatg acccggtact
1561tggagtcctg gggggcagcc aagccctttg cacacctaca tcacagggac agcatgacca
1621cagagaacgg caagatcagc accacggatg tacccatggg tcagcataat tttcaaaatc
1681gcaccttaag aaccaagtca caaaagaaga gatttgaaga agaattgaat gaaaggatga
1741ttcaagcaat tgatgggatt aatgcacaga agcaatggct caagtctgaa gacattcaga
1801gaatctcact gcttttctat aacaaagtac tagaaaaaga gtaccgggcc acggcactgc
1861cagcgttcaa gtattatgtg acttgtgcct gtctcatatt cttctgcatc ttcattgtgc
1921agattctcgt gctgccaaaa acgtctgtcc tgggcatctc ctttggggct gcgtttctct
1981tgctggcctt catcctcttc gtctgctttg ctggacagct tctgcaatgc agcaaaaaag
2041cctctcccct gctcatgtgg cttttgaagt cctcgggcat cattgccaac cgcccctggc
2101cacggatctc tctcacgatc atcaccacag ccatcatatt aatgatggcc gtgttcaaca
2161tgtttttcct gagtgactca gaggaaacaa tccctccaac tgccaacaca acaaacacaa
2221gcttttcagc ctcaaataat caggtggcga ttctgcgtgc gcagaattta tttttcctcc
2281cgtactttat ctacagctgc attctgggac tgatatcctg ttccgtgttc ctgcgggtaa
2341actatgagct gaagatgttg atcatgatgg tggccttggt gggctacaac accatcctac
2401tccacaccca cgcccacgtc ctgggcgact acagccaggt cttatttgag agaccaggca
2461tttggaaaga cctgaagacc atgggctctg tgtctctctc tatattcttc atcacactgc
2521ttgttctggg tagacagaat gaatattact gtaggttaga cttcttatgg aagaacaaat
2581tcaaaaaaga gcgggaggag atagagacca tggagaacct gaaccgcgtg ctgctggaga
2641acgtgcttcc cgcgcacgtg gctgagcact tcctggccag gagcctgaag aatgaggagc
2701tataccacca gtcctatgac tgcgtctgtg tcatgtttgc ctccattccg gatttcaaag
2761aattttatac agaatccgac gtgaacaagg agggcttgga atgccttcgg ctcctgaacg
2821agatcatcgc tgactttgat gatcttcttt ccaagccaaa attcagtgga gttgaaaaga
2881ttaagaccat tggcagcaca tacatggcag caacaggtct gagcgctgtg cccagccagg
2941agcactccca ggagcccgag cggcagtaca tgcacattgg caccatggtg gagtttgctt
3001ttgccctggt agggaagctg gatgccatca acaagcactc cttcaacgac ttcaaattgc
3061gagtgggtat taaccatgga cctgtgatag ctggtgtgat tggagctcag aagccacaat
3121atgatatctg gggcaacact gtcaatgtgg ccagtaggat ggacagcacc ggagtcctgg
3181acaaaataca ggttaccgag gagacgagcc tcgtcctgca gaccctcgga tacacgtgca
3241cctgtcgagg aataatcaac gtgaaaggaa agggggacct gaagacgtac tttgtaaaca
3301cagaaatgtc aaggtccctt tcccagagca acgtggcatc cttgccaact ttcttgtaca
3361aagtggttgg taagcctatc cctaaccctc tcctcggtct cgattctacg tagtaatgag
3421ctagcgctaa ccggtggcgc gttaagtcga caatcaacct ctggatta

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