Construct: ORF ccsbBroad304_05786
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009578.1_s304c1, BRDN0000404622
- Derived from:
- ccsbBroadEn_05786
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ADRB2 (154)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_05786
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 154 | ADRB2 | adrenoceptor beta 2 | NM_000024.5 | 99.8% | 99.5% | 46A>G;79C>G |
| 2 | mouse | 11555 | Adrb2 | adrenergic receptor, beta 2 | NM_007420.3 | 84.7% | 87% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1305
- ORF length:
- 1239
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatggg gcaacccggg aacggcagcg ccttcttgct ggcacccaat ggaagccatg 121 cgccggacca cgacgtcacg caggaaaggg acgaggtgtg ggtggtgggc atgggcatcg 181 tcatgtctct catcgtcctg gccatcgtgt ttggcaatgt gctggtcatc acagccattg 241 ccaagttcga gcgtctgcag acggtcacca actacttcat cacttcactg gcctgtgctg 301 atctggtcat gggcctggca gtggtgccct ttggggccgc ccatattctt atgaaaatgt 361 ggacttttgg caacttctgg tgcgagtttt ggacttccat tgatgtgctg tgcgtcacgg 421 ccagcattga gaccctgtgc gtgatcgcag tggatcgcta ctttgccatt acttcacctt 481 tcaagtacca gagcctgctg accaagaata aggcccgggt gatcattctg atggtgtgga 541 ttgtgtcagg ccttacctcc ttcttgccca ttcagatgca ctggtaccgg gccacccacc 601 aggaagccat caactgctat gccaatgaga cctgctgtga cttcttcacg aaccaagcct 661 atgccattgc ctcttccatc gtgtccttct acgttcccct ggtgatcatg gtcttcgtct 721 actccagggt ctttcaggag gccaaaaggc agctccagaa gattgacaaa tctgagggcc 781 gcttccatgt ccagaacctt agccaggtgg agcaggatgg gcggacgggg catggactcc 841 gcagatcttc caagttctgc ttgaaggagc acaaagccct caagacgtta ggcatcatca 901 tgggcacttt caccctctgc tggctgccct tcttcatcgt taacattgtg catgtgatcc 961 aggataacct catccgtaag gaagtttaca tcctcctaaa ttggataggc tatgtcaatt 1021 ctggtttcaa tccccttatc tactgccgga gcccagattt caggattgcc ttccaggagc 1081 ttctgtgcct gcgcaggtct tctttgaagg cctatgggaa tggctactcc agcaacggca 1141 acacagggga gcagagtgga tatcacgtgg aacaggagaa agaaaataaa ctgctgtgtg 1201 aagacctccc aggcacggaa gactttgtgg gccatcaagg tactgtgcct agcgataaca 1261 ttgattcaca agggaggaat tgtagtacaa atgactcact gctgtaccca actttcttgt 1321 acaaagtggt tggtaagcct atccctaacc ctctcctcgg tctcgattct acgtagtaat 1381 gagctagcgc taaccggtgg cgcgttaagt cgacaatcaa cctctggatt a