Transcript: Mouse NM_007420.3

Mus musculus adrenergic receptor, beta 2 (Adrb2), mRNA.

Source:
NCBI, updated 2017-06-22
Taxon:
Mus musculus (mouse)
Gene:
Adrb2 (11555)
Length:
2269
CDS:
230..1486

Additional Resources:

NCBI RefSeq record:
NM_007420.3
NBCI Gene record:
Adrb2 (11555)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_007420.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000418476 CAAATGACTCGCCACTGTAAT pLKO_005 1467 CDS 100% 13.200 18.480 N Adrb2 n/a
2 TRCN0000412379 TGTCAATATCGTGCACGTTAT pLKO_005 1102 CDS 100% 10.800 15.120 N Adrb2 n/a
3 TRCN0000414086 ACTGTATTTGAGTGCTTATTG pLKO_005 1661 3UTR 100% 13.200 9.240 N Adrb2 n/a
4 TRCN0000027525 CCTCATCCCTAAGGAAGTTTA pLKO.1 1132 CDS 100% 13.200 9.240 N Adrb2 n/a
5 TRCN0000027563 CCTCTTTGTATGGAACTTAAA pLKO.1 1702 3UTR 100% 13.200 9.240 N Adrb2 n/a
6 TRCN0000027532 GCCAGTCACATCCTTATGAAA pLKO.1 500 CDS 100% 5.625 3.938 N Adrb2 n/a
7 TRCN0000027556 CCCACAAGAAAGCTATCGATT pLKO.1 759 CDS 100% 4.950 3.465 N Adrb2 n/a
8 TRCN0000027549 CGGCTACTCTAGCAATAGCAA pLKO.1 1285 CDS 100% 3.000 2.100 N Adrb2 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_007420.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_05786 pDONR223 100% 84.7% 87% None (many diffs) n/a
2 ccsbBroad304_05786 pLX_304 0% 84.7% 87% V5 (many diffs) n/a
3 TRCN0000488338 AACCACCCTAACTTCCAACATAGA pLX_317 24.6% 84.6% 86.6% V5 (many diffs) n/a
4 TRCN0000488268 CTACATTCCGGTATCAGCTAAGTC pLX_317 24.8% 84.6% 86.8% V5 (not translated due to prior stop codon) (many diffs) n/a
5 TRCN0000491269 AAATCCCGCTTGTTCCGGCGGATA pLX_317 24.9% 84.5% 86.3% V5 (not translated due to prior stop codon) (many diffs) n/a
6 TRCN0000488071 TACATCTGCGGGACACGCTTTAGC pLX_317 24.8% 84.5% 86.3% V5 (not translated due to prior stop codon) (many diffs) n/a
7 TRCN0000487831 CGTCCATAACCCTTAACCCGCTTT pLX_317 21.1% 84.4% 86.1% V5 (many diffs) n/a
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