Construct: ORF ccsbBroad304_05812
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011419.1_s304c1, BRDN0000400175
- Derived from:
- ccsbBroadEn_05812
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- AMY2B (280)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_05812
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 280 | AMY2B | amylase alpha 2B | NM_020978.4 | 99.8% | 99.6% | 9C>A;1510G>C;1512A>T |
| 2 | human | 279 | AMY2A | amylase alpha 2A | NM_000699.3 | 98.1% | 98.4% | (many diffs) |
| 3 | human | 277 | AMY1B | amylase alpha 1B | NM_001008218.1 | 97.3% | 97.4% | (many diffs) |
| 4 | human | 278 | AMY1C | amylase alpha 1C | NM_001008219.2 | 97.3% | 97.4% | (many diffs) |
| 5 | human | 276 | AMY1A | amylase alpha 1A | NM_001008221.1 | 97.3% | 97.4% | (many diffs) |
| 6 | human | 278 | AMY1C | amylase alpha 1C | NM_001346780.1 | 97.3% | 97.4% | (many diffs) |
| 7 | human | 276 | AMY1A | amylase alpha 1A | NM_004038.3 | 97.3% | 97.4% | (many diffs) |
| 8 | human | 277 | AMY1B | amylase alpha 1B | XM_011541262.1 | 97.3% | 97.4% | (many diffs) |
| 9 | human | 278 | AMY1C | amylase alpha 1C | XM_017001058.1 | 97.3% | 97.4% | (many diffs) |
| 10 | mouse | 109959 | Amy2a5 | amylase 2a5 | NM_001042711.2 | 83.6% | 84.3% | (many diffs) |
| 11 | mouse | 100043684 | Amy2a4 | amylase 2a4 | NM_001160150.1 | 83.6% | 84.3% | (many diffs) |
| 12 | mouse | 100043686 | Amy2a3 | amylase 2a3 | NM_001160151.1 | 83.6% | 84.3% | (many diffs) |
| 13 | mouse | 100043688 | Amy2a2 | amylase 2a2 | NM_001160152.1 | 83.6% | 84.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1599
- ORF length:
- 1533
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgaa gttatttctg ttgcttttca ccattgggtt ctgctgggct cagtattccc 121 caaatacaca acaaggacgg acatctattg ttcatctgtt tgaatggcga tgggttgata 181 ttgctcttga atgtgagcga tatttagctc ccaagggatt tggaggggtt caggtctctc 241 caccaaatga aaatgttgca attcacaacc ctttcagacc ttggtgggaa agataccaac 301 cagttagcta taaattatgc acaagatctg gaaatgaaga tgaatttaga aacatggtga 361 ctagatgtaa caatgttggg gttcgtattt atgtggatgc tgtaattaat catatgtctg 421 gtaatgctgt gagtgcagga acaagcagta cctgtggaag ttacttcaac cctggaagta 481 gggactttcc agcagtccca tattctggat gggattttaa tgatggtaaa tgtaaaactg 541 gaagtggaga tatcgagaac tacaatgatg ctactcaggt cagagattgt cgtctggttg 601 gtcttcttga tcttgcactg gagaaagatt atgtgcgttc caagattgcc gaatatatga 661 atcatctcat tgacattggt gttgcagggt tcagacttga tgcttccaag cacatgtggc 721 ctggagacat aaaggcaatt ttggacaaac tgcataatct aaacagtaac tggttccctg 781 caggaagtaa acctttcatt taccaggagg taattgatct gggtggtgag ccaattaaaa 841 gcagtgacta ctttggaaat ggccgggtga cagaattcaa gtatggtgca aaactcggca 901 cagttattcg caagtggaat ggagagaaga tgtcttacct aaagaactgg ggagaaggtt 961 ggggtttcat gccttctgac agagcacttg tctttgtgga taaccatgac aatcaacgag 1021 gacatggggc tggaggagcc tctattctta ccttctggga tgctaggctg tataaaatgg 1081 cagttggatt tatgcttgct catccttatg gttttacacg agtaatgtca agctaccgtt 1141 ggccaagaca gtttcaaaat ggaaacgatg ttaatgattg ggttgggcca ccaaataata 1201 atggagtaat taaagaagtt actattaatc cagacactac ttgtggcaat gactgggtct 1261 gtgaacatcg atggcgccaa ataaggaaca tggttaattt ccgcaatgta gtggatggcc 1321 agccttttac aaactggtat gataatggga gcaaccaagt ggcttttggg agaggaaaca 1381 gaggattcat tgttttcaac aatgatgact ggacattttc tttaactttg caaactggtc 1441 ttcctgctgg cacatactgt gatgtcattt ctggagataa aattaatggc aattgcacag 1501 gcattaaaat ctacgtttct gacgatggca aagctcattt ttctattagt aactctgctg 1561 aggatccatt tattcctatt catgctgaat ctaaattata cccaactttc ttgtacaaag 1621 tggttggtaa gcctatccct aaccctctcc tcggtctcga ttctacgtag taatgagcta 1681 gcgctaaccg gtggcgcgtt aagtcgacaa tcaacctctg gatta