Transcript: Mouse NM_001042711.2

Mus musculus amylase 2a5 (Amy2a5), mRNA.

Source:
NCBI, updated 2017-05-14
Taxon:
Mus musculus (mouse)
Gene:
Amy2a5 (109959)
Length:
1577
CDS:
18..1544

Additional Resources:

NCBI RefSeq record:
NM_001042711.2
NBCI Gene record:
Amy2a5 (109959)

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_001042711.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000256686 CAATGTTGGTGTCCGTATTTA pLKO_005 323 CDS 100% 15.000 7.500 Y Amy2a5 n/a
2 TRCN0000250553 CTGGGTGGTGAGGCAATTAAA pLKO_005 762 CDS 100% 15.000 7.500 Y Amy2a5 n/a
3 TRCN0000250554 CTTGGGACTTTAACGATAATA pLKO_005 460 CDS 100% 15.000 7.500 Y Amy2a5 n/a
4 TRCN0000256687 GCGAGAAGATGTCCTATTTAA pLKO_005 865 CDS 100% 15.000 7.500 Y Amy2a5 n/a
5 TRCN0000258060 GGCGAGAAGATGTCCTATTTA pLKO_005 864 CDS 100% 15.000 7.500 Y Amy2a5 n/a
6 TRCN0000347951 TGGGACTTTAACGATAATAAA pLKO_005 462 CDS 100% 15.000 7.500 Y Amy2a2 n/a
7 TRCN0000348025 TTACCGTTGGAATAGAAATTT pLKO_005 1076 CDS 100% 15.000 7.500 Y Amy2a3 n/a
8 TRCN0000255965 TTCAAACTGGTGGGATAATAA pLKO_005 1271 CDS 100% 15.000 7.500 Y Amy2a5 n/a
9 TRCN0000255969 TTGGGACTTTAACGATAATAA pLKO_005 461 CDS 100% 15.000 7.500 Y Amy2a5 n/a
10 TRCN0000348024 TTTCAAACTGGTGGGATAATA pLKO_005 1270 CDS 100% 15.000 7.500 Y Amy2a4 n/a
11 TRCN0000250555 ACAATGTTGGTGTCCGTATTT pLKO_005 322 CDS 100% 13.200 6.600 Y Amy2a5 n/a
12 TRCN0000348022 ACCAAGGTGGCTGACTATATG pLKO_005 582 CDS 100% 13.200 6.600 Y Amy2a4 n/a
13 TRCN0000348030 ACTCTGCTGAGGACCCATTTA pLKO_005 1495 CDS 100% 13.200 6.600 Y Amy2a2 n/a
14 TRCN0000255966 ATACCAACCAATCAGCTATAA pLKO_005 245 CDS 100% 13.200 6.600 Y Amy2a5 n/a
15 TRCN0000255967 GAGTGGCGCTGGGTTGATATT pLKO_005 114 CDS 100% 13.200 6.600 Y Amy2a5 n/a
16 TRCN0000176801 GATGCTTATCAGGTCAGAAAT pLKO.1 510 CDS 100% 13.200 6.600 Y Amy2a5 n/a
17 TRCN0000256690 GCTTGGGACTTTAACGATAAT pLKO_005 459 CDS 100% 13.200 6.600 Y Amy2a5 n/a
18 TRCN0000255968 GGGTTCTGCTGGGCTCAATAT pLKO_005 48 CDS 100% 13.200 6.600 Y Amy2a5 n/a
19 TRCN0000256689 TGAACACAGATGGCGTCAAAT pLKO_005 1205 CDS 100% 13.200 6.600 Y Amy2a5 n/a
20 TRCN0000347943 TGGCGAGAAGATGTCCTATTT pLKO_005 863 CDS 100% 13.200 6.600 Y Amy2a4 n/a
21 TRCN0000348026 CAGATGGGAGGACTGCTATTG pLKO_005 82 CDS 100% 10.800 5.400 Y Amy2a3 n/a
22 TRCN0000364003 GATACCAACCAATCAGCTATA pLKO_005 244 CDS 100% 10.800 5.400 Y Amy2a4 n/a
23 TRCN0000347948 TGAACCATCTCATTGACATTG pLKO_005 601 CDS 100% 10.800 5.400 Y Amy2a3 n/a
24 TRCN0000348028 TGCTTGGGACTTTAACGATAA pLKO_005 458 CDS 100% 10.800 5.400 Y Amy2a3 n/a
25 TRCN0000348029 TGGTGACAAGGTGCAACAATG pLKO_005 307 CDS 100% 10.800 5.400 Y Amy2a2 n/a
26 TRCN0000348031 TTAAAGGTAGTGAGTACTTTG pLKO_005 778 CDS 100% 10.800 5.400 Y Amy2a2 n/a
27 TRCN0000181494 GCAACAATGTTGGTGTCCGTA pLKO.1 319 CDS 100% 2.640 1.320 Y Amy2a5 n/a
28 TRCN0000250552 TACCAACCAATCAGCTATAAA pLKO_005 246 CDS 100% 0.000 0.000 Y Amy2a5 n/a
29 TRCN0000256688 TACCAAGGTGGCTGACTATAT pLKO_005 581 CDS 100% 0.000 0.000 Y Amy2a5 n/a
30 TRCN0000056025 GCACATACTGTGATGTCATTT pLKO.1 1393 CDS 100% 13.200 6.600 Y AMY2A n/a
31 TRCN0000151403 GCACATACTGTGATGTCATTT pLKO.1 1393 CDS 100% 13.200 6.600 Y AMY1C n/a
32 TRCN0000056026 CGTATTTATGTGGATGCTGTA pLKO.1 336 CDS 100% 4.050 2.025 Y AMY2A n/a
Download CSV

shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001042711.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

Download CSV

All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_05813 pDONR223 100% 83.9% 84.7% None (many diffs) n/a
2 ccsbBroad304_05813 pLX_304 0% 83.9% 84.7% V5 (many diffs) n/a
3 TRCN0000471163 CACTTCGGTCTGCGCATCTCCTTG pLX_317 27.3% 83.9% 84.7% V5 (many diffs) n/a
4 ccsbBroadEn_00065 pDONR223 100% 83.8% 85.1% None (many diffs) n/a
5 ccsbBroad304_00065 pLX_304 0% 83.8% 85.1% V5 (many diffs) n/a
6 TRCN0000472453 AACGACGCGTATCCGTTCGTTCAA pLX_317 30.1% 83.8% 85.1% V5 (many diffs) n/a
7 ccsbBroadEn_05812 pDONR223 100% 83.6% 84.3% None (many diffs) n/a
8 ccsbBroad304_05812 pLX_304 0% 83.6% 84.3% V5 (many diffs) n/a
9 TRCN0000472633 ACTCACATTAATATATTCTTGGCG pLX_317 30.1% 83.6% 84.3% V5 (many diffs) n/a
Download CSV

GPP Web Portal Terms of Service

Effective Date: December 8, 2025
By using this site, you agree to our terms and conditions below.

Overview of Terms

The data made available on this website were generated for research purposes and are not intended for clinical or commercial uses. Commercial use (or other use for profit-making purposes) of the GPP Web Portal and its tools, is not permitted under these terms and may require a separate license agreement from Broad or its contributors. For more information, please contact partnering@broadinstitute.org.

The original data may be subject to rights claimed by third parties, including but not limited to, patent, copyright, other intellectual property rights, biodiversity-related access and benefit-sharing rights. It is the responsibility of users of Broad Institute services to ensure that their use of the data does not infringe any of the rights of such third parties.

Any questions or comments concerning these Terms of Use can be addressed to: legal@broadinstitute.org.

By accessing and viewing this GPP Web Portal, you agree to the following terms and conditions:

Attribution

You agree to acknowledge the Broad Institute (e.g., in publications, services or products) for any of your use of its online services, databases or software in accordance with good scientific practice. You agree to use the acknowledgment wording provided for the relevant tools as indicated on the FAQ for each tool.

Updating the Terms of Use

We reserve the right to update these Terms of Use at any time. When alterations are inevitable, we will attempt to give reasonable notice of any changes by placing a notice on our website, but you may wish to check each time you use the website. The date of the most recent revision will appear on this, the "GPP Web Portal Terms of Use" page. If you do not agree to these changes, please do not continue to use our online services. We will also make available an archived copy of the previous Terms of Use for comparison.

Indemnification and Disclaimer of Warranties

You are using this GPP Web Portal at your own risk, and you hereby agree to hold Broad and its contributors and their trustees, directors, officers, employees, and affiliated investigators harmless for any third party claims which may arise from your use of the GPP Web Portal, the tools available therein, or any portion thereof. Further, you agree to indemnify Broad, its contributors, and its and their trustees, directors, officers, employees, affiliated investigators, students, and affiliates for any loss, costs, claims, damages, or other liabilities arising from any unpermitted commercial or profit-making use you make of the GPP Web Portal. The GPP Web Portal is a research tool and is provided "as is". Broad does not represent that the GPP Web Portal is free of errors or bugs or suitable for any particular tasks.

ANY EXPRESS OR IMPLIED WARRANTIES, INCLUDING, BUT NOT LIMITED TO, THE IMPLIED WARRANTIES OF MERCHANTABILITY, FITNESS FOR A PARTICULAR PURPOSE, NONINFRINGEMENT, OR THE ABSENCE OF LATENT OR OTHER DEFECTS ARE DISCLAIMED. IN NO EVENT SHALL BROAD OR ITS CONTRIBUTORS BE LIABLE FOR ANY DIRECT, INDIRECT, INCIDENTAL, SPECIAL, EXEMPLARY, OR CONSEQUENTIAL DAMAGES HOWEVER CAUSED AND ON ANY THEORY OF LIABILITY, WHETHER IN CONTRACT, STRICT LIABILITY, OR TORT (INCLUDING NEGLIGENCE OR OTHERWISE) ARISING IN ANY WAY OUT OF THE USE OF THE GPP WEB PORTAL, EVEN IF ADVISED OF THE POSSIBILITY OF SUCH DAMAGE.

Governing Law

The terms and conditions herein shall be construed, governed, interpreted, and applied in accordance with the internal laws of the Commonwealth of Massachusetts, U.S.A. Furthermore, by accessing, downloading, or using the Database, You consent to the personal jurisdiction of, and venue in, the state and federal courts within Massachusetts with respect to Your download or use of the Database.