Construct: ORF ccsbBroad304_05863
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000864.1_s304c1, BRDN0000407057
- Derived from:
- ccsbBroadEn_05863
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ATF3 (467)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_05863
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 467 | ATF3 | activating transcription fa... | NM_001030287.3 | 99.8% | 99.4% | 533C>A |
| 2 | human | 467 | ATF3 | activating transcription fa... | NM_001674.4 | 99.8% | 99.4% | 533C>A |
| 3 | human | 467 | ATF3 | activating transcription fa... | XM_005273146.1 | 99.8% | 99.4% | 533C>A |
| 4 | human | 467 | ATF3 | activating transcription fa... | XM_011509579.1 | 99.8% | 99.4% | 533C>A |
| 5 | human | 467 | ATF3 | activating transcription fa... | NM_001206484.3 | 68.3% | 67.9% | 0_1ins171;362C>A |
| 6 | human | 467 | ATF3 | activating transcription fa... | NM_001206488.3 | 68.3% | 67.9% | 0_1ins171;362C>A |
| 7 | human | 467 | ATF3 | activating transcription fa... | NM_001040619.3 | 68.2% | 65.5% | (many diffs) |
| 8 | human | 467 | ATF3 | activating transcription fa... | NM_001206486.2 | 52.6% | 50% | (many diffs) |
| 9 | human | 467 | ATF3 | activating transcription fa... | XR_001737203.1 | 18.2% | (many diffs) | |
| 10 | mouse | 11910 | Atf3 | activating transcription fa... | NM_007498.3 | 88.7% | 94.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 609
- ORF length:
- 543
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 tgggcatgat gcttcaacac ccaggccagg tctctgcctc ggaagtgagt gcttctgcca 121 tcgtcccctg cctgtcccct cctgggtcac tggtgtttga ggattttgct aacctgacgc 181 cctttgtcaa ggaagagctg aggtttgcca tccagaacaa gcacctctgc caccggatgt 241 cctctgcgct ggaatcagtc actgtcagcg acagacccct cggggtgtcc atcacaaaag 301 ccgaggtagc ccctgaagaa gatgaaagga aaaagaggcg acgagaaaga aataagattg 361 cagctgcaaa gtgccgaaac aagaagaagg agaagacgga gtgcctgcag aaagagtcgg 421 agaagctgga aagtgtgaat gctgaactga aggctcagat tgaggagctc aagaacgaga 481 agcagcattt gatatacatg ctcaaccttc atcggcccac gtgtattgtc cgggctcaga 541 atgggaggac tccagaagat gagagaaacc tctttatcca acagataaaa gaaggaaaat 601 tgcagagcta cccaactttc ttgtacaaag tggttggtaa gcctatccct aaccctctcc 661 tcggtctcga ttctacgtag taatgagcta gcgctaaccg gtggcgcgtt aagtcgacaa 721 tcaacctctg gatta