Construct: ORF ccsbBroad304_05893
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011284.1_s304c1, BRDN0000462393
- Derived from:
- ccsbBroadEn_05893
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- BLM (641)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_05893
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 641 | BLM | BLM RecQ like helicase | NM_000057.4 | 99.9% | 99.9% | 3157G>A |
| 2 | human | 641 | BLM | BLM RecQ like helicase | NM_001287246.2 | 99.9% | 99.9% | 3157G>A |
| 3 | human | 641 | BLM | BLM RecQ like helicase | NM_001287247.2 | 90.7% | 90.6% | 3157G>A;3357_3358ins393 |
| 4 | human | 641 | BLM | BLM RecQ like helicase | NM_001287248.2 | 73.5% | 73.4% | 0_1ins1125;2032G>A |
| 5 | human | 641 | BLM | BLM RecQ like helicase | XM_011521881.2 | 69% | 69% | 0_1ins1314;1843G>A |
| 6 | human | 641 | BLM | BLM RecQ like helicase | XM_011521882.3 | 62.4% | 60.3% | (many diffs) |
| 7 | human | 641 | BLM | BLM RecQ like helicase | XM_006720632.2 | 53.8% | 53.7% | 0_1ins1962;1195G>A |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 4320
- ORF length:
- 4251
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat ggctgctgtt cctcaaaata atctacagga gcaactagaa cgtcactcag 121 ccagaacact taataataaa ttaagtcttt caaaaccaaa attttcaggt ttcactttta 181 aaaagaaaac atcttcagat aacaatgtat ctgtaactaa tgtgtcagta gcaaaaacac 241 ctgtattaag aaataaagat gttaatgtta ccgaagactt ttccttcagt gaacctctac 301 ccaacaccac aaatcagcaa agggtcaagg acttctttaa aaatgctcca gcaggacagg 361 aaacacagag aggtggatca aaatcattat tgccagattt cttgcagact ccgaaggaag 421 ttgtatgcac tacccaaaac acaccaactg taaagaaatc ccgggatact gctctcaaga 481 aattagaatt tagttcttca ccagattctt taagtaccat caatgattgg gatgatatgg 541 atgactttga tacttctgag acttcaaaat catttgttac accaccccaa agtcactttg 601 taagagtaag cactgctcag aaatcaaaaa agggtaagag aaactttttt aaagcacagc 661 tttatacaac aaacacagta aagactgatt tgcctccacc ctcctctgaa agcgagcaaa 721 tagatttgac tgaggaacag aaggatgact cagaatggtt aagcagcgat gtgatttgca 781 tcgatgatgg ccccattgct gaagtgcata taaatgaaga tgctcaggaa agtgactctc 841 tgaaaactca tttggaagat gaaagagata atagcgaaaa gaagaagaat ttggaagaag 901 ctgaattaca ttcaactgag aaagttccat gtattgaatt tgatgatgat gattatgata 961 cggattttgt tccaccttct ccagaagaaa ttatttctgc ttcttcttcc tcttcaaaat 1021 gccttagtac gttaaaggac cttgacacct ctgacagaaa agaggatgtt cttagcacat 1081 caaaagatct tttgtcaaaa cctgagaaaa tgagtatgca ggagctgaat ccagaaacca 1141 gcacagactg tgacgctaga cagataagtt tacagcagca gcttattcat gtgatggagc 1201 acatctgtaa attaattgat actattcctg atgataaact gaaacttttg gattgtggga 1261 acgaactgct tcagcagcgg aacataagaa ggaaacttct aacggaagta gattttaata 1321 aaagtgatgc cagtcttctt ggctcattgt ggagatacag gcctgattca cttgatggcc 1381 ctatggaggg tgattcctgc cctacaggga attctatgaa ggagttaaat ttttcacacc 1441 ttccctcaaa ttctgtttct cctggggact gtttactgac taccacccta ggaaagacag 1501 gattctctgc caccaggaag aatctttttg aaaggccttt attcaatacc catttacaga 1561 agtcctttgt aagtagcaac tgggctgaaa caccaagact aggaaaaaaa aatgaaagct 1621 cttatttccc aggaaatgtt ctcacaagca ctgctgtgaa agatcagaat aaacatactg 1681 cttcaataaa tgacttagaa agagaaaccc aaccttccta tgatattgat aattttgaca 1741 tagatgactt tgatgatgat gatgactggg aagacataat gcataattta gcagccagca 1801 aatcttccac agctgcctat caacccatca aggaaggtcg gccaattaaa tcagtatcag 1861 aaagactttc ctcagccaag acagactgtc ttccagtgtc atctactgct caaaatataa 1921 acttctcaga gtcaattcag aattatactg acaagtcagc acaaaattta gcatccagaa 1981 atctgaaaca tgagcgtttc caaagtctta gttttcctca tacaaaggaa atgatgaaga 2041 tttttcataa aaaatttggc ctgcataatt ttagaactaa tcagctagag gcgatcaatg 2101 ctgcactgct tggtgaagac tgttttatcc tgatgccgac tggaggtggt aagagtttgt 2161 gttaccagct ccctgcctgt gtttctcctg gggtcactgt tgtcatttct cccttgagat 2221 cacttatcgt agatcaagtc caaaagctga cttccttgga tattccagct acatatctga 2281 caggtgataa gactgactca gaagctacaa atatttacct ccagttatca aaaaaagacc 2341 caatcataaa acttctatat gtcactccag aaaagatctg tgcaagtaac agactcattt 2401 ctactctgga gaatctctat gagaggaagc tcttggcacg ttttgttatt gatgaagcac 2461 attgtgtcag tcagtgggga catgattttc gtcaagatta caaaagaatg aatatgcttc 2521 gccagaagtt tccttctgtt ccggtgatgg ctcttacggc cacagctaat cccagggtac 2581 agaaggacat cctgactcag ctgaagattc tcagacctca ggtgtttagc atgagcttta 2641 acagacataa tctgaaatac tatgtattac cgaaaaagcc taaaaaggtg gcatttgatt 2701 gcctagaatg gatcagaaag caccacccat atgattcagg gataatttac tgcctctcca 2761 ggcgagaatg tgacaccatg gctgacacgt tacagagaga tgggctcgct gctcttgctt 2821 accatgctgg cctcagtgat tctgccagag atgaagtgca gcagaagtgg attaatcagg 2881 atggctgtca ggttatctgt gctacaattg catttggaat ggggattgac aaaccggacg 2941 tgcgatttgt gattcatgca tctctcccta aatctgtgga gggttactac caagaatctg 3001 gcagagctgg aagagatggg gaaatatctc actgcctgct tttctatacc tatcatgatg 3061 tgaccagact gaaaagactt ataatgatgg aaaaagatgg aaaccatcat acaagagaaa 3121 ctcacttcaa taatttgtat agcatggtac attactgtga aaatataacg gaatgcagga 3181 gaatacagct tttggcctac tttggtgaaa atggatttaa tcctaatttt tgtaagaaac 3241 acccagatgt ttcttgtgat aattgctgta aaacaaagga ttataaaaca agagatgtga 3301 ctgacgatgt gaaaagtatt gtaagatttg ttcaagaaca tagttcatca caaggaatga 3361 gaaatataaa acatgtaggt ccttctggaa gatttactat gaatatgctg gtcgacattt 3421 tcttggggag taagagtgca aaaatccagt caggtatatt tggaaaagga tctgcttatt 3481 cacgacacaa tgccgaaaga ctttttaaaa agctgatact tgacaagatt ttggatgaag 3541 acttatatat caatgccaat gaccaggcga tcgcttatgt gatgctcgga aataaagccc 3601 aaactgtact aaatggcaat ttaaaggtag actttatgga aacagaaaat tccagcagtg 3661 tgaaaaaaca aaaagcgtta gtagcaaaag tgtctcagag ggaagagatg gttaaaaaat 3721 gtcttggaga acttacagaa gtctgcaaat ctctggggaa agtttttggt gtccattact 3781 tcaatatttt taataccgtc actctcaaga agcttgcaga atctttatct tctgatcctg 3841 aggttttgct tcaaattgat ggtgttactg aagacaaact ggaaaaatat ggtgcggaag 3901 tgatttcagt attacagaaa tactctgaat ggacatcgcc agctgaagac agttccccag 3961 ggataagcct gtccagcagc agaggccccg gaagaagtgc cgctgaggag ctcgacgagg 4021 aaatacccgt atcttcccac tactttgcaa gtaaaaccag aaatgaaagg aagaggaaaa 4081 agatgccagc ctcccaaagg tctaagagga gaaaaactgc ttccagtggt tccaaggcaa 4141 agggggggtc tgccacatgt agaaagatat cttccaaaac gaaatcctcc agcatcattg 4201 gatccagttc agcctcacat acttctcaag cgacatcagg agccaatagc aaattgggga 4261 ttatggctcc accgaagcct ataaatagac cgtttcttaa gccttcatat gcattctcat 4321 tgccaacttt cttgtacaaa gtggttggta agcctatccc taaccctctc ctcggtctcg 4381 attctacgta gtaatgagct agcgctaacc ggtggcgcgt taagtcgaca atcaacctct 4441 ggatta