Construct: ORF ccsbBroad304_05903
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016499.1_s304c1, BRDN0000407649
- Derived from:
- ccsbBroadEn_05903
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- BPI (671)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_05903
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 671 | BPI | bactericidal permeability i... | NM_001725.3 | 99% | 98.7% | 1_1delAinsATGAGAGAGAACT;634G>A |
| 2 | human | 671 | BPI | bactericidal permeability i... | XM_024451972.1 | 51.3% | 50.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1527
- ORF length:
- 1461
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgag agagaacttg gccaggggcc cttgcaacgc gccgagatgg gcgtccctga 121 tggtgctggt cgccataggc accgccgtga cagcggccgt caaccctggc gtcgtggtca 181 ggatctccca gaagggcctg gactacgcca gccagcaggg gacggccgct ctgcagaagg 241 agctgaagag gatcaagatt cctgactact cagacagctt taagatcaag catcttggga 301 aggggcatta tagcttctac agcatggaca tccgtgaatt ccagcttccc agttcccaga 361 taagcatggt gcccaatgtg ggccttaagt tctccatcag caacgccaat atcaagatca 421 gcgggaaatg gaaggcacaa aagagattct taaaaatgag cggcaatttt gacctgagca 481 tagaaggcat gtccatttcg gctgatctga agctgggcag taaccccacg tcaggcaagc 541 ccaccatcac ctgctccagc tgcagcagcc acatcaacag tgtccacgtg cacatctcaa 601 agagcaaagt ggggtggctg atccaactct tccacaaaaa aattgagtct gcgcttcgaa 661 acaagatgaa cagccaggtc tgcgagaaag tgaccaattc tgtatcctcc aagctgcaac 721 cttatttcca gactctgcca gtaatgacca aaatagattc tgtggctgga atcaactatg 781 gtctggtggc acctccagca accacggctg agaccctgga tgtacagatg aagggggagt 841 tttacagtga gaaccaccac aatccacctc cctttgctcc accagtgatg gagtttcccg 901 ctgcccatga ccgcatggta tacctgggcc tctcagacta cttcttcaac acagccgggc 961 ttgtatacca agaggctggg gtcttgaaga tgacccttag agatgacatg attccaaagg 1021 agtccaaatt tcgactgaca accaagttct ttggaacctt cctacctgag gtggccaaga 1081 agtttcccaa catgaagata cagatccatg tctcagcctc caccccgcca cacctgtctg 1141 tgcagcccac cggccttacc ttctaccctg ccgtggatgt ccaggccttt gccgtcctcc 1201 ccaactcctc cctggcttcc ctcttcctga ttggcatgca cacaactggt tccatggagg 1261 tcagcgccga gtccaacagg cttgttggag agctcaagct ggataggctg ctcctggaac 1321 tgaagcactc aaatattggc cccttcccgg ttgaattgct gcaggatatc atgaactaca 1381 ttgtacccat tcttgtgctg cccagggtta acgagaaact acagaaaggc ttccctctcc 1441 cgacgccggc cagagtccag ctctacaacg tagtgcttca gcctcaccag aacttcctgc 1501 tgttcggtgc agacgttgtc tataaatgcc caactttctt gtacaaagtg gttggtaagc 1561 ctatccctaa ccctctcctc ggtctcgatt ctacgtagta atgagctagc gctaaccggt 1621 ggcgcgttaa gtcgacaatc aacctctgga tta