Construct: ORF ccsbBroad304_05925
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001450.1_s304c1, BRDN0000461501
- Derived from:
- ccsbBroadEn_05925
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CACNB3 (784)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_05925
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 784 | CACNB3 | calcium voltage-gated chann... | NM_000725.4 | 99.8% | 100% | 555C>A;1131C>G |
| 2 | human | 784 | CACNB3 | calcium voltage-gated chann... | NM_001206916.2 | 97.4% | 96.5% | (many diffs) |
| 3 | human | 784 | CACNB3 | calcium voltage-gated chann... | NM_001206917.2 | 96.9% | 96.9% | (many diffs) |
| 4 | human | 784 | CACNB3 | calcium voltage-gated chann... | NM_001206915.2 | 91.3% | 91.5% | 166_167ins123;432C>A;1008C>G |
| 5 | human | 784 | CACNB3 | calcium voltage-gated chann... | XM_011538727.3 | 63.4% | 63.6% | 0_1ins528;27C>A;603C>G |
| 6 | human | 784 | CACNB3 | calcium voltage-gated chann... | XM_011538728.2 | 63.4% | 63.6% | 0_1ins528;27C>A;603C>G |
| 7 | human | 784 | CACNB3 | calcium voltage-gated chann... | XM_017019957.1 | 63.4% | 63.6% | 0_1ins528;27C>A;603C>G |
| 8 | human | 784 | CACNB3 | calcium voltage-gated chann... | XM_024449180.1 | 63.4% | 63.6% | 0_1ins528;27C>A;603C>G |
| 9 | human | 784 | CACNB3 | calcium voltage-gated chann... | XM_024449181.1 | 63.4% | 63.6% | 0_1ins528;27C>A;603C>G |
| 10 | human | 784 | CACNB3 | calcium voltage-gated chann... | XR_001748868.1 | 53.9% | (many diffs) | |
| 11 | human | 784 | CACNB3 | calcium voltage-gated chann... | XR_001748867.1 | 52.1% | (many diffs) | |
| 12 | mouse | 12297 | Cacnb3 | calcium channel, voltage-de... | NM_007581.3 | 93.5% | 99.1% | (many diffs) |
| 13 | mouse | 12297 | Cacnb3 | calcium channel, voltage-de... | XM_017316415.1 | 92.4% | 97.9% | (many diffs) |
| 14 | mouse | 12297 | Cacnb3 | calcium channel, voltage-de... | XM_006520361.2 | 92.2% | 97.7% | (many diffs) |
| 15 | mouse | 12297 | Cacnb3 | calcium channel, voltage-de... | NM_001044741.2 | 91.2% | 95.7% | (many diffs) |
| 16 | mouse | 12297 | Cacnb3 | calcium channel, voltage-de... | XM_017316416.1 | 91% | 95.5% | (many diffs) |
| 17 | mouse | 12297 | Cacnb3 | calcium channel, voltage-de... | XM_017316417.1 | 90.7% | 96% | (many diffs) |
| 18 | mouse | 12297 | Cacnb3 | calcium channel, voltage-de... | XM_006520363.2 | 90.6% | 95.5% | (many diffs) |
| 19 | mouse | 12297 | Cacnb3 | calcium channel, voltage-de... | XM_006520360.1 | 90.1% | 94.5% | (many diffs) |
| 20 | mouse | 12297 | Cacnb3 | calcium channel, voltage-de... | XM_006520364.2 | 90% | 94.8% | (many diffs) |
| 21 | mouse | 12297 | Cacnb3 | calcium channel, voltage-de... | XM_006520362.3 | 89.9% | 94.3% | (many diffs) |
| 22 | mouse | 12297 | Cacnb3 | calcium channel, voltage-de... | XM_006520365.1 | 89.5% | 94.8% | (many diffs) |
| 23 | mouse | 12297 | Cacnb3 | calcium channel, voltage-de... | XM_017316418.1 | 89.5% | 94.8% | (many diffs) |
| 24 | mouse | 12297 | Cacnb3 | calcium channel, voltage-de... | NM_001286226.1 | 88.2% | 93.5% | (many diffs) |
| 25 | mouse | 12297 | Cacnb3 | calcium channel, voltage-de... | XM_006520367.1 | 57.8% | 62.2% | (many diffs) |
| 26 | mouse | 12297 | Cacnb3 | calcium channel, voltage-de... | XM_017316419.1 | 57.8% | 62.2% | (many diffs) |
| 27 | mouse | 12297 | Cacnb3 | calcium channel, voltage-de... | XM_006520370.1 | 44.5% | 47.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1518
- ORF length:
- 1452
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgta tgacgactcc tacgtgcccg ggtttgagga ctcggaggcg ggttcagccg 121 actcctacac cagccgccca tctctggact cagacgtctc cctggaggag gaccgggaga 181 gtgcccggcg tgaagtagag agccaggctc agcagcagct cgaaagggcc aagcacaaac 241 ctgtggcatt tgcggtgagg accaatgtca gctactgtgg cgtactggat gaggagtgcc 301 cagtccaggg ctctggagtc aactttgagg ccaaagattt tctgcacatt aaagagaagt 361 acagcaatga ctggtggatc gggcggctag tgaaagaggg cggggacatc gccttcatcc 421 ccagccccca gcgcctggag agcatccggc tcaaacagga gcagaaggcc aggagatctg 481 ggaacccttc cagcctgagt gacattggca accgacgctc ccctccgcca tctctagcca 541 agcagaagca aaagcaggcg gaacatgttc ccccatatga cgtggtgccc tccatgcggc 601 ctgtggtgct ggtgggacca tctctgaaag gttatgaggt cacagacatg atgcagaagg 661 ctctcttcga cttcctcaaa cacagatttg atggcaggat ctccatcacc cgagtcacag 721 ccgacctctc cctggcaaag cgatctgtgc tcaacaatcc gggcaagagg accatcattg 781 agcgctcctc tgcccgctcc agcattgcgg aagtgcagag tgagatcgag cgcatatttg 841 agctggccaa atccctgcag ctagtagtgt tggacgctga caccatcaac cacccagcac 901 agctggccaa gacctcgctg gcccccatca tcgtctttgt caaagtgtcc tcaccaaagg 961 tactccagcg tctcattcgc tcccggggga agtcacagat gaagcacctg accgtacaga 1021 tgatggcata tgataagctg gttcagtgcc caccggagtc atttgatgtg attctggatg 1081 agaaccagct ggaggatgcc tgtgagcacc tggctgagta cctggaggtt tactggcggg 1141 ccacgcacca cccagcccct ggccccggac ttctgggtcc tcccagtgcc atcccgggac 1201 ttcagaacca gcagctgctg ggggagcgtg gcgaggagca ctcccccctt gagcgggaca 1261 gcttgatgcc ctctgatgag gccagcgaga gctcccgcca agcctggaca ggatcttcac 1321 agcgtagctc ccgccacctg gaggaggact atgcagatgc ctaccaggac ctgtaccagc 1381 ctcaccgcca acacacctcg gggctgccta gtgctaacgg gcatgacccc caagaccggc 1441 ttctagccca ggactcagag cacaaccaca gtgaccggaa ctggcagcgc aaccggcctt 1501 ggcccaagga tagctactgc ccaactttct tgtacaaagt ggttggtaag cctatcccta 1561 accctctcct cggtctcgat tctacgtagt aatgagctag cgctaaccgg tggcgcgtta 1621 agtcgacaat caacctctgg atta