Construct: ORF ccsbBroad304_06022
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014104.1_s304c1, BRDN0000407340
- Derived from:
- ccsbBroadEn_06022
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- COX7B (1349)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_06022
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1349 | COX7B | cytochrome c oxidase subuni... | NM_001866.3 | 99.5% | 100% | 12G>A |
| 2 | mouse | 66142 | Cox7b | cytochrome c oxidase subuni... | NM_025379.2 | 85.4% | 78.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 306
- ORF length:
- 240
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgtt tcccttagtc aaaagcgcac taaatcgtct ccaagttcga agcattcagc 121 aaacaatggc aaggcagagc caccagaaac gtacacctga ttttcatgac aaatacggta 181 atgctgtatt agctagtgga gccactttct gtattgttac atggacatat gtagcaacac 241 aagtcggaat agaatggaac ctgtcccctg ttggcagagt taccccaaag gaatggagga 301 atcagtaccc aactttcttg tacaaagtgg ttggtaagcc tatccctaac cctctcctcg 361 gtctcgattc tacgtagtaa tgagctagcg ctaaccggtg gcgcgttaag tcgacaatca 421 acctctggat ta