Construct: ORF ccsbBroad304_06040
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007697.1_s304c1, BRDN0000410199
- Derived from:
- ccsbBroadEn_06040
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CRY1 (1407)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_06040
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1407 | CRY1 | cryptochrome circadian regu... | NM_004075.5 | 99.9% | 100% | 636T>C |
| 2 | human | 1407 | CRY1 | cryptochrome circadian regu... | XM_017018832.2 | 93.8% | 91.3% | (many diffs) |
| 3 | human | 1407 | CRY1 | cryptochrome circadian regu... | XM_024448844.1 | 92.6% | 87.2% | 636T>C;1656_1729del;1833_1896del |
| 4 | human | 1407 | CRY1 | cryptochrome circadian regu... | XM_024448845.1 | 86.9% | 79.3% | (many diffs) |
| 5 | mouse | 12952 | Cry1 | cryptochrome 1 (photolyase-... | NM_007771.3 | 86.3% | 93.3% | (many diffs) |
| 6 | mouse | 12952 | Cry1 | cryptochrome 1 (photolyase-... | XM_011243348.1 | 79% | 83.7% | (many diffs) |
| 7 | mouse | 12952 | Cry1 | cryptochrome 1 (photolyase-... | XM_011243349.1 | 62.2% | 67.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1824
- ORF length:
- 1758
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatggg ggtgaacgcc gtgcactggt tccgaaaggg gctccggctc cacgacaacc 121 ccgccctgaa ggagtgcatt cagggcgccg acaccatccg ctgcgtctac atcctggacc 181 cctggttcgc cggctcctcc aatgtgggca tcaacaggtg gcgatttttg cttcagtgtc 241 ttgaggatct tgatgccaat ctacgaaaat taaactcccg tctgtttgtg attcgtggac 301 aaccagcaga tgtgtttccc aggcttttca aggaatggaa cattactaaa ctttcaattg 361 agtatgattc tgagcccttt ggaaaggaac gagacgcagc tattaagaaa ctggcaactg 421 aagctggagt agaagtcatt gtaagaattt cacatacatt atatgaccta gacaagatca 481 tagaactcaa tggtggacaa ccgcctctaa cttataaaag attccagact ctcatcagca 541 aaatggaacc actagagata ccagtagaga caattacttc agaagtgata gaaaagtgca 601 caactcctct gtctgatgac catgatgaga aatatggagt cccttcactg gaagagctag 661 gttttgatac agatggctta tcctctgcag tgtggccagg cggagaaact gaagcactta 721 ctcgtttgga aaggcatttg gaaagaaaag cttgggtggc aaattttgaa agacctcgaa 781 tgaatgcgaa ttctctgctt gcaagcccta ctggacttag tccttatctc cgatttggtt 841 gtttgtcatg tcgactgttt tacttcaaac taacagatct ctacaaaaag gtaaagaaga 901 acagttcccc tcccctttcc ctttatgggc aactgttatg gcgtgaattt ttctatacag 961 cagcaacaaa taatccacgc tttgataaaa tggaaggaaa ccctatctgt gttcagattc 1021 cttgggataa aaatcctgag gctttagcca aatgggcgga aggccggaca ggctttccat 1081 ggattgatgc catcatgaca cagcttcgtc aggagggttg gattcatcat ctagccaggc 1141 atgcagttgc ttgcttcctG ACACGAGGGG ACCTGTGGAT TAGTTGGGAA GAAGGAATGA 1201 AGGTATTTGA AGAATTATTG CTTGATGCAG ATTGGAGCAT AAATGCTGGA AGTTGGATGT 1261 GGCTGTCTTG TAGTTCCTTT TTTCAACAGT TTTTTCACTG CTATTGCCCT GTTGGTTTTG 1321 GTAGGAGAAC AGATCCCAAT GGAGACTATA TCAGGCGTTA TTTGCCTGTC CTAAGAGGCT 1381 TCCCTGCAAA ATATATCTAT GATCCCTGGA ATGCACCAGA AGGTATCCAA AAGGTAGCCA 1441 AATGTTTGAT AGGAGTTAAT TATCCTAAAC CAATGGTGAA CCATGCTGAG GCAAGCCGTT 1501 TGAATATCGA AAGGATGAAA CAGATCTATC AGCAGCTTTC ACGATATAGA GGACTAGGTC 1561 TTCTGGCATC AGTACCTTCT AATCCTAATG GGAATGGAGG CTTCATGGGA TATTCTGCAG 1621 AAAATATCCC AGGTTGTAGC AGCAGTGGAA GTTGCTCTCA AGGGAGTGGT ATTTTACACT 1681 ATGCTCATGG CGACAGTCAG CAAACTCACC TGTTGAAGCA AGGAAGAAGC TCCATGGGCA 1741 CTGGTCTCAG TGGTGGGAAA CGTCCTAGTC AGGAAGAGGA CACACAGAGT ATTGGTCCTA 1801 AAGTCCAGAG ACAGAGCACT AATTACCCAA CTTTCTTGTA Caaagtggtt ggtaagccta 1861 tccctaaccc tctcctcggt ctcgattcta cgtagtaatg agctagcgct aaccggtggc 1921 gcgttaagtc gacaatcaac ctctggatta