Transcript: Human NM_004075.5

Homo sapiens cryptochrome circadian regulator 1 (CRY1), mRNA.

Source:
NCBI, updated 2019-09-27
Taxon:
Homo sapiens (human)
Gene:
CRY1 (1407)
Length:
2987
CDS:
589..2349

Additional Resources:

NCBI RefSeq record:
NM_004075.5
NBCI Gene record:
CRY1 (1407)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_004075.5, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000231066 GGACAACCGCCTCTAACTTAT pLKO_005 1018 CDS 100% 13.200 18.480 N CRY1 n/a
2 TRCN0000011306 CCGTTTGAATATCGAAAGGAT pLKO.1 2019 CDS 100% 3.000 4.200 N CRY1 n/a
3 TRCN0000011307 GCAACTGTTATGGCGTGAATT pLKO.1 1452 CDS 100% 0.000 0.000 N CRY1 n/a
4 TRCN0000231067 AGACTATATCAGGCGTTATTT pLKO_005 1866 CDS 100% 15.000 12.000 N CRY1 n/a
5 TRCN0000231068 CCAGAGACAGAGCACTAATTA pLKO_005 2328 CDS 100% 15.000 12.000 N CRY1 n/a
6 TRCN0000231065 GGAACGAGACGCAGCTATTAA pLKO_005 909 CDS 100% 15.000 10.500 N CRY1 n/a
7 TRCN0000218592 GCGTAATCCAAATTGGTATTG pLKO_005 2808 3UTR 100% 10.800 7.560 N CRY1 n/a
8 TRCN0000194462 GAGGATCTTGATGCCAATCTA pLKO.1 766 CDS 100% 5.625 3.938 N Cry1 n/a
9 TRCN0000011308 GACAAGATCATAGAACTCAAT pLKO.1 994 CDS 100% 4.950 3.465 N CRY1 n/a
10 TRCN0000011305 GCAACAAATAATCCACGCTTT pLKO.1 1486 CDS 100% 4.050 2.835 N CRY1 n/a
11 TRCN0000011304 CCTCTGTTAACACAGACAGTA pLKO.1 2832 3UTR 100% 0.495 0.347 N CRY1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_004075.5, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_06040 pDONR223 100% 99.9% 100% None 636T>C n/a
2 ccsbBroad304_06040 pLX_304 37.9% 99.9% 100% V5 636T>C n/a
3 TRCN0000466669 ACCGCCTCACAAACATCCCGGGCA pLX_317 11.6% 99.9% 100% V5 636T>C n/a
4 TRCN0000489831 CATATGCTTGTGTTTTGGCCGGAA pLX_317 22.2% 99.9% 100% V5 636T>C n/a
5 TRCN0000492211 TAGTACAGGGTCGCGACACTTACG pLX_317 20.9% 99.9% 100% V5 (not translated due to prior stop codon) 636T>C n/a
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