Construct: ORF ccsbBroad304_06042

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF009410.1_s304c1, BRDN0000410315
Derived from:
ccsbBroadEn_06042
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
CRYAB (1410)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_06042
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 1410 CRYAB crystallin alpha B NM_001289807.1 99.8% 100% 213G>A
2 human 1410 CRYAB crystallin alpha B NM_001289808.2 99.8% 100% 213G>A
3 human 1410 CRYAB crystallin alpha B NM_001368245.1 99.8% 100% 213G>A
4 human 1410 CRYAB crystallin alpha B NM_001885.3 99.8% 100% 213G>A
5 human 1410 CRYAB crystallin alpha B NM_001330379.1 61.5% 61.7% 0_1ins201;12G>A
6 human 1410 CRYAB crystallin alpha B NM_001368246.1 61.5% 61.7% 0_1ins201;12G>A
7 mouse 12955 Cryab crystallin, alpha B NM_001289782.1 91% 97.7% (many diffs)
8 mouse 12955 Cryab crystallin, alpha B NM_001289784.1 91% 97.7% (many diffs)
9 mouse 12955 Cryab crystallin, alpha B NM_001289785.1 91% 97.7% (many diffs)
10 mouse 12955 Cryab crystallin, alpha B NM_009964.3 91% 97.7% (many diffs)
11 mouse 12955 Cryab crystallin, alpha B XM_006509970.3 91% 97.7% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
591
ORF length:
525
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatgga catcgccatc caccacccct ggatccgccg ccccttcttt cctttccact
121cccccagccg cctctttgac cagttcttcg gagagcacct gttggagtct gatcttttcc
181cgacgtctac ttccctgagt cccttctacc ttcggccacc ctccttcctg cgggcaccca
241gctggtttga cactggactc tcagagatgc gcctggaaaa ggacaggttc tctgtcaacc
301tggatgtgaa gcacttctcc ccagaggaac tcaaagttaa ggtgttggga gatgtgattg
361aggtgcatgg aaaacatgaa gagcgccagg atgaacatgg tttcatctcc agggagttcc
421acaggaaata ccggatccca gctgatgtag accctctcac cattacttca tccctgtcat
481ctgatggggt cctcactgtg aatggaccaa ggaaacaggt ctctggccct gagcgcacca
541ttcccatcac ccgtgaagag aagcctgctg tcaccgcagc ccccaagaaa tacccaactt
601tcttgtacaa agtggttggt aagcctatcc ctaaccctct cctcggtctc gattctacgt
661agtaatgagc tagcgctaac cggtggcgcg ttaagtcgac aatcaacctc tggatta

Download FASTA (ORF) (Full)