Transcript: Human NM_001330379.1

Homo sapiens crystallin alpha B (CRYAB), transcript variant 4, mRNA.

Source:
NCBI, updated 2019-09-16
Taxon:
Homo sapiens (human)
Gene:
CRYAB (1410)
Length:
571
CDS:
84..410

Additional Resources:

NCBI RefSeq record:
NM_001330379.1
NBCI Gene record:
CRYAB (1410)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001330379.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000003840 CCATTACTTCATCCCTGTCAT pLKO.1 277 CDS 100% 4.950 6.930 N CRYAB n/a
2 TRCN0000433426 CTCTGTCAACCTGGATGTGAA pLKO_005 107 CDS 100% 4.950 3.465 N CRYAB n/a
3 TRCN0000010822 GAAGAGCGCCAGGATGAACAT pLKO.1 195 CDS 100% 4.950 3.465 N CRYAB n/a
4 TRCN0000434713 TGTGATTGAGGTGCATGGAAA pLKO_005 170 CDS 100% 4.950 3.465 N CRYAB n/a
5 TRCN0000003841 GCAGGCCCAAATTATCAAGCT pLKO.1 507 3UTR 100% 2.640 1.848 N CRYAB n/a
6 TRCN0000097212 TCACTGTGAATGGACCAAGGA pLKO.1 310 CDS 100% 2.640 1.848 N Cryab n/a
7 TRCN0000317169 TCACTGTGAATGGACCAAGGA pLKO_005 310 CDS 100% 2.640 1.848 N Cryab n/a
8 TRCN0000010823 CCGTGAAGAGAAGCCTGCTGT pLKO.1 368 CDS 100% 0.880 0.616 N CRYAB n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001330379.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_06042 pDONR223 100% 61.5% 61.7% None 0_1ins201;12G>A n/a
2 ccsbBroad304_06042 pLX_304 0% 61.5% 61.7% V5 0_1ins201;12G>A n/a
3 TRCN0000480151 ATTACTATCTTGTGATTCGACGGA pLX_317 71.7% 61.5% 61.7% V5 0_1ins201;12G>A n/a
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