Construct: ORF ccsbBroad304_06091

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF017022.1_s304c1, BRDN0000400315
Derived from:
ccsbBroadEn_06091
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
DDX1 (1653)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_06091
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 1653 DDX1 DEAD-box helicase 1 NM_004939.3 99.9% 100% 912C>T
2 mouse 104721 Ddx1 DEAD (Asp-Glu-Ala-Asp) box ... NM_134040.1 90.1% 97.5% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
2286
ORF length:
2220
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatggc ggccttctcc gagatgggtg taatgcctga gattgcacaa gctgtggaag
121agatggattg gctcctccca actgatatcc aggctgaatc tatcccattg atcttaggag
181gaggtgatgt acttatggct gcagaaacag gaagtggcaa aactggtgct tttagtattc
241cagttatcca gatagtttat gaaactctga aagaccaaca ggaaggcaaa aaaggaaaaa
301caacaattaa aactggtgct tcagtgctga acaaatggca gatgaaccca tatgacagag
361gatctgcttt tgcaattggg tcagatggtc tttgttgtca aagcagagaa gtaaaggaat
421ggcatgggtg tagagctact aaaggattaa tgaaagggaa acactactat gaagtatcct
481gtcatgacca agggttatgc agggtcgggt ggtctaccat gcaggcctct ttggacctag
541gtactgacaa gtttggattt ggctttggtg gaacaggaaa gaaatcccat aacaaacaat
601ttgataatta tggagaggaa ttcactatgc atgataccat tggatgttac ctggatatag
661ataagggaca tgtcaagttc tccaaaaatg gaaaagatct tggtctggca tttgaaatac
721caccacatat gaaaaaccaa gccctctttc ctgcctgtgt tttgaagaat gctgaactga
781aatttaactt cggtgaagag gaatttaagt ttccaccaaa agatggcttt gttgctcttt
841ccaaggcacc ggatggttac attgtcaaat cacagcactc aggtaatgca caggtgacac
901aaacaaagtt tctccccaat gctccgaaag ctctcattgt tgaaccttcc cgggagttag
961ctgaacaaac tttgaataac atcaagcagt ttaagaaata cattgataat cctaaattaa
1021gggagcttct gataattgga ggtgttgcag cccgggatca gctctctgtt ttggaaaatg
1081gagtagatat agttgtaggt actccgggaa gactagatga cttggtgtca actggaaagc
1141tgaacttatc tcaagttaga ttcctggtcc tggatgaagc tgatgggctt ctttctcaag
1201gttattctga ttttataaat aggatgcaca atcagattcc tcaggttacc tctgatggaa
1261aaagacttca ggtgattgtt tgctctgcca ctttgcattc tttcgatgta aagaaactgt
1321ccgagaagat aatgcatttt cctacatggg ttgacttaaa aggagaagac tctgttccag
1381atactgtaca ccatgttgtt gtcccagtaa atcccaaaac tgacagactc tgggaaaggc
1441ttggaaagag ccacattaga actgatgatg tacatgcaaa agataacaca agacctggtg
1501ctaatagtcc agagatgtgg tctgaagcta ttaaaatcct gaaaggggag tatgctgtcc
1561gggcaatcaa ggaacataag atggatcaag caattatctt ctgtagaacc aaaattgact
1621gtgataactt ggagcagtac tttatacaac aaggaggagg acctgataaa aaaggacacc
1681agttctcatg tgtttgtctt catggtgaca gaaagcctca tgagagaaag caaaacttgg
1741aaagatttaa gaaaggagat gtaagattct tgatttgcac agatgtagct gctagaggaa
1801ttgatatcca cggtgttcct tatgttataa atgtcactct gcccgatgaa aagcaaaact
1861acgtacatcg aattggcaga gtaggaagag ctgaaaggat gggtctggca atttccctgg
1921tggcaacaga aaaagaaaag gtttggtacc atgtatgtag cagccgtgga aaagggtgtt
1981ataacacaag actcaaggaa gatggaggct gtaccatatg gtacaacgag atgcagttac
2041tatctgagat agaagaacac ctgaactgta ccatttctca ggttgagccg gatataaagg
2101taccagtgga tgaatttgat gggaaagtta cctacggtca gaaaagggct gctggtggtg
2161gaagctataa aggccatgtg gatattttgg cacctactgt tcaagagttg gctgcccttg
2221aaaaggaggc gcagacatct ttcctgcatc ttggctacct tcctaaccag ctgttcagaa
2281ccttctgccc aactttcttg tacaaagtgg ttggtaagcc tatccctaac cctctcctcg
2341gtctcgattc tacgtagtaa tgagctagcg ctaaccggtg gcgcgttaag tcgacaatca
2401acctctggat ta

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