Construct: ORF ccsbBroad304_06104

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF013669.1_s304c1, BRDN0000462374
Derived from:
ccsbBroadEn_06104
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
SARDH (1757)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_06104
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 1757 SARDH sarcosine dehydrogenase NM_001134707.2 99.9% 99.8% 1222G>T
2 human 1757 SARDH sarcosine dehydrogenase NM_007101.4 99.9% 99.8% 1222G>T
3 human 1757 SARDH sarcosine dehydrogenase XM_017014367.1 95.6% 95.4% 1222G>T;2632_2633insT;2634_2635ins119
4 human 1757 SARDH sarcosine dehydrogenase XM_017014368.1 90.6% 90.5% (many diffs)
5 human 1757 SARDH sarcosine dehydrogenase XR_001746217.1 83.1% (many diffs)
6 human 1757 SARDH sarcosine dehydrogenase XR_001746218.1 82.6% (many diffs)
7 human 1757 SARDH sarcosine dehydrogenase XR_001746216.1 76.4% 1_296del;1518G>T;3051_3601del
8 human 1757 SARDH sarcosine dehydrogenase XR_929726.2 76.1% 1_167del;1389G>T;2922_3613del
9 human 1757 SARDH sarcosine dehydrogenase XR_001746213.1 73.5% 1_296del;1518G>T;3051_3742del
10 human 1757 SARDH sarcosine dehydrogenase XR_001746215.1 73.5% 1_298del;1520G>T;3053_3744del
11 human 1757 SARDH sarcosine dehydrogenase XR_002956762.1 56.9% (many diffs)
12 human 1757 SARDH sarcosine dehydrogenase XR_001746214.1 55.8% 1_1479del;2701G>T;4234_4925del
13 mouse 192166 Sardh sarcosine dehydrogenase NM_138665.2 84.9% 88.4% (many diffs)
14 mouse 192166 Sardh sarcosine dehydrogenase XM_006497788.3 84.9% 88.4% (many diffs)
15 mouse 192166 Sardh sarcosine dehydrogenase XM_006497789.3 84.9% 88.4% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
2823
ORF length:
2754
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcaccat ggcctcactg agccgagccc tacgtgtggc tgctgcccac cctcgccaga
121gccctacccg gggcatgggg ccatgcaacc tgtccagcgc agctggcccc acagccgaga
181agagtgtgcc atatcagcgg accctgaagg agggacaggg cacctcggtg gtggcccaag
241gcccaagccg gcccctgccc agcacggcca acgtggtggt cattggtgga ggcagcttgg
301gctgccagac cctgtaccac ctggccaagc tgggcatgag tggggcggtg ctgctggagc
361gggagcggct gacctccggg accacctggc acacggcagg cctgctgtgg cagctgcggc
421ccagtgacgt ggaggtggag cttctggccc acactcggcg ggtggtgagc cgggagctgg
481aggaggagac gggactacac acgggctgga tccagaatgg gggcctcttc atcgcgtcca
541accggcagcg cctggacgag tacaagaggc tcatgtcgct gggcaaggcg tatggtgtgg
601aatcccatgt gctgagcccg gcagagacca agactctgta cccgctgatg aatgtggacg
661acctctacgg gaccctgtat gtgccgcacg acggtaccat ggaccccgct ggcacctgta
721ccaccctcgc cagggcagct tctgcccgag gagcacaggt cattgagaac tgcccagtga
781ccggcattcg tgtgtggacg gatgattttg gggtgcggcg ggtcgcgggt gtggagactc
841agcatggttc catccagaca ccctgcgtgg tcaactgtgc aggagtgtgg gcaagtgctg
901tgggccggat ggctggagtc aaggtcccgc tggtggccat gcaccatgcc tatgtcgtca
961ccgagcgcat cgaggggatt cagaacatgc ccaatgtccg tgatcatgat gcctctgtct
1021acctccgcct ccaaggggat gccttgtctg tgggtggcta tgaggccaac cccatctttt
1081gggaggaggt gtcagacaag tttgccttcg gcctctttga cctggactgg gaggtgttca
1141cccagcacat tgaaggcgcc atcaacaggg tccccgtgct ggagaagaca ggaatcaagt
1201ccacggtctg cggccctgaa tccttcacgc ccgaccacaa gcccctgatg ggggaggcac
1261ctgagctccg agggttcttc ctgggctgtt gcttcaacag cgcaggaatg atgctgggtg
1321gtggctgtgg gcaggagctg gcccactgga tcatccatgg gcgcccggag aaggacatgc
1381atggctatga catcaggcgc ttccatcact cgctcacgga ccacccccgc tggatccgag
1441agcgaagcca tgagtcctac gccaagaact actccgtcgt cttcccccac gatgagccgc
1501tggccgggcg caacatgagg agagacccgc tgcacgagga actccttgga caaggctgcg
1561tgttccagga gcggcatggc tgggagcgac cgggatggtt tcatccccga ggcccagctc
1621cggtcctcga gtacgactac tacggggctt acgggagccg cgcgcacgag gactacgcct
1681accgcaggct gctggcagac gagtacacct tcgccttccc gccccaccac gacacgatca
1741agaaggagtg cctggcctgc agaggggccg ccgctgtgtt tgacatgtcc tacttcggga
1801agttctacct ggtggggctg gatgcaagga aggctgccga ctggctcttc tccgcagatg
1861tcagccgacc cccaggctcc accgtgtaca cgtgcatgct caaccaccgt gggggcaccg
1921agagtgacct gactgtcagc cgcctggcac ccagccacca ggcctccccg ctggcccccg
1981cctttgaagg ggacggttac tacctggcca tgggcggggc cgtggcccag cacaactggt
2041cccacatcac caccgtgctg caggaccaga agtcccagtg ccagctcatc gacagctccg
2101aggacctggg tatgatcagt atccagggcc cagccagccg agccattttg caggaggtgc
2161tggacgcaga cctgagcaac gaggccttcc cgttctccac ccacaagcta ctgagagccg
2221cagggcacct ggtccgagcc atgcggctgt cctttgtggg ggagctgggc tgggagctgc
2281acattccaaa ggcgtcctgc gtgcctgtgt accgggctgt gatggccgcg ggtgccaagc
2341acggcctcat caacgcaggg taccgcgcca tcgactccct gagcattgag aaaggctacc
2401ggcactggca cgcggacctg cggccagacg acagccccct ggaggcaggc ctggccttca
2461cctgcaagct caagtcgccg gtgcccttcc tggggaggga ggccctggag cagcagcggg
2521ccgcaggcct ccgccggcgc ctggtgtgct tcaccatgga ggacaaagta cccatgtttg
2581gcctggaggc catctggagg aacggccaag tggtgggcca tgtccggagg gctgactttg
2641ggttcgccat cgacaagacc atcgcctacg gttacatcca tgaccccagc ggtgggccgg
2701tctcgctgga ctttgtgaag agcggggact atgccctgga gagaatgggg gtgacctatg
2761gtgcccaggc tcacctgaag tcgcccttcg accccaacaa caagagggtg aagggaatct
2821acttgccaac tttcttgtac aaagtggttg gtaagcctat ccctaaccct ctcctcggtc
2881tcgattctac gtagtaatga gctagcgcta accggtggcg cgttaagtcg acaatcaacc
2941tctggatta

Download FASTA (ORF) (Full)