Transcript: Mouse NM_138665.2

Mus musculus sarcosine dehydrogenase (Sardh), mRNA.

Source:
NCBI, updated 2017-05-28
Taxon:
Mus musculus (mouse)
Gene:
Sardh (192166)
Length:
4573
CDS:
673..3432

Additional Resources:

NCBI RefSeq record:
NM_138665.2
NBCI Gene record:
Sardh (192166)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_138665.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000041409 CGATGAGTACACCTTCGACTT pLKO.1 2304 CDS 100% 4.050 5.670 N Sardh n/a
2 TRCN0000041412 GCAAGGCCTATGGTATAGAAT pLKO.1 1190 CDS 100% 5.625 4.500 N Sardh n/a
3 TRCN0000041408 GCAGAGACCAAGAGTCTATAT pLKO.1 1228 CDS 100% 13.200 9.240 N Sardh n/a
4 TRCN0000041411 CGGAGAGCTGACTTTGGATTT pLKO.1 3232 CDS 100% 10.800 7.560 N Sardh n/a
5 TRCN0000041410 CACCGAGAAGAGTGTTCCTTA pLKO.1 777 CDS 100% 4.950 3.465 N Sardh n/a
Download CSV

shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_138665.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

Download CSV

All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_06104 pDONR223 100% 84.9% 88.4% None (many diffs) n/a
2 ccsbBroad304_06104 pLX_304 0% 84.9% 88.4% V5 (many diffs) n/a
3 TRCN0000475878 ATGTTTCTAACCAGAGTCAGCAGC pLX_317 12.6% 84.9% 88.4% V5 (many diffs) n/a
Download CSV