Construct: ORF ccsbBroad304_06113
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002567.1_s304c1, BRDN0000462182
- Derived from:
- ccsbBroadEn_06113
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- DNM2 (1785)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_06113
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1785 | DNM2 | dynamin 2 | NM_001005360.2 | 99.9% | 100% | 2139T>C |
| 2 | human | 1785 | DNM2 | dynamin 2 | NM_001190716.2 | 99.8% | 99.8% | 2139T>C;2540_2541insCAG |
| 3 | human | 1785 | DNM2 | dynamin 2 | NM_004945.3 | 99.5% | 99.5% | 1544_1545insGGGGGAGATCCT;2127T>C |
| 4 | human | 1785 | DNM2 | dynamin 2 | NM_001005361.2 | 98.1% | 98.5% | (many diffs) |
| 5 | human | 1785 | DNM2 | dynamin 2 | NM_001005362.2 | 97.6% | 98% | (many diffs) |
| 6 | mouse | 13430 | Dnm2 | dynamin 2 | NM_001253893.1 | 89.3% | 98.1% | (many diffs) |
| 7 | mouse | 13430 | Dnm2 | dynamin 2 | XM_006509979.1 | 89.2% | 98% | (many diffs) |
| 8 | mouse | 13430 | Dnm2 | dynamin 2 | NM_007871.2 | 88.9% | 97.7% | (many diffs) |
| 9 | mouse | 13430 | Dnm2 | dynamin 2 | XM_017313127.1 | 88.8% | 97.5% | (many diffs) |
| 10 | mouse | 13430 | Dnm2 | dynamin 2 | XM_006509973.1 | 88.3% | 97% | (many diffs) |
| 11 | mouse | 13430 | Dnm2 | dynamin 2 | XM_006509977.1 | 88.3% | 96.7% | (many diffs) |
| 12 | mouse | 13430 | Dnm2 | dynamin 2 | XM_006509975.1 | 88.2% | 96.9% | (many diffs) |
| 13 | mouse | 13430 | Dnm2 | dynamin 2 | NM_001039520.2 | 88.1% | 96.6% | (many diffs) |
| 14 | mouse | 13430 | Dnm2 | dynamin 2 | XM_006509976.2 | 87.9% | 96.5% | (many diffs) |
| 15 | mouse | 13430 | Dnm2 | dynamin 2 | XM_006509980.2 | 87.8% | 96.3% | (many diffs) |
| 16 | mouse | 13430 | Dnm2 | dynamin 2 | XM_017313125.1 | 87.8% | 96.4% | (many diffs) |
| 17 | mouse | 13430 | Dnm2 | dynamin 2 | XM_017313128.1 | 87.7% | 96.2% | (many diffs) |
| 18 | mouse | 13430 | Dnm2 | dynamin 2 | NM_001253894.1 | 87.5% | 96.4% | (many diffs) |
| 19 | mouse | 13430 | Dnm2 | dynamin 2 | XM_006509974.1 | 87.3% | 95.6% | (many diffs) |
| 20 | mouse | 13430 | Dnm2 | dynamin 2 | XM_006509982.2 | 87.1% | 95.9% | (many diffs) |
| 21 | mouse | 13430 | Dnm2 | dynamin 2 | XM_006509978.2 | 86.5% | 95.3% | (many diffs) |
| 22 | mouse | 13430 | Dnm2 | dynamin 2 | XM_006509981.2 | 86.5% | 95% | (many diffs) |
| 23 | mouse | 13430 | Dnm2 | dynamin 2 | XM_017313126.1 | 86.1% | 94.8% | (many diffs) |
| 24 | mouse | 13430 | Dnm2 | dynamin 2 | XM_017313129.1 | 86% | 94.5% | (many diffs) |
| 25 | mouse | 13430 | Dnm2 | dynamin 2 | XM_006509983.1 | 71.6% | 79.2% | (many diffs) |
| 26 | mouse | 13430 | Dnm2 | dynamin 2 | XM_006509984.3 | 52.5% | 57% | (many diffs) |
| 27 | mouse | 13430 | Dnm2 | dynamin 2 | XM_006509985.3 | 46.1% | 49.7% | (many diffs) |
| 28 | mouse | 13430 | Dnm2 | dynamin 2 | XR_001778793.1 | 44.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2676
- ORF length:
- 2610
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatggg caaccgcggg atggaagagc tgatcccgct ggtcaacaaa ctgcaggacg 121 ccttcagctc catcggccag agctgccacc tggacctgcc gcagatcgct gtagtgggcg 181 gccagagcgc cggcaagagc tcggtgctgg agaacttcgt gggccgggac ttccttcccc 241 gcggttcagg aatcgtcacc cggcggcctc tcattctgca gctcatcttc tcaaaaacag 301 aacatgccga gtttttgcac tgcaagtcca aaaagtttac agactttgat gaagtccggc 361 aggagattga agcagagacc gacagggtca cggggaccaa caaaggcatc tccccagtgc 421 ccatcaacct tcgagtctac tcgccacacg tgttgaactt gaccctcatc gacctcccgg 481 gtatcaccaa ggtgcctgtg ggcgaccagc ctccagacat cgagtaccag atcaaggaca 541 tgatcctgca gttcatcagc cgggagagca gcctcattct ggctgtcacg cccgccaaca 601 tggacctggc caactccgac gccctcaagc tggccaagga agtcgatccc caaggcctac 661 ggaccatcgg tgtcatcacc aagcttgacc tgatggacga gggcaccgac gccagggacg 721 tcttggagaa caagttgctc ccgttgagaa gaggctacat tggcgtggtg aaccgcagcc 781 agaaggatat tgagggcaag aaggacatcc gtgcagcact ggcagctgag aggaagttct 841 tcctctccca cccggcctac cggcacatgg ccgaccgcat gggcacgcca catctgcaga 901 agacgctgaa tcagcaactg accaaccaca tccgggagtc gctgccggcc ctacgtagca 961 aactacagag ccagctgctg tccctggaga aggaggtgga ggagtacaag aactttcggc 1021 ccgacgaccc cacccgcaaa accaaagccc tgctgcagat ggtccagcag tttggggtgg 1081 attttgagaa gaggatcgag ggctcaggag atcaggtgga cactctggag ctctccgggg 1141 gcgcccgaat caatcgcatc ttccacgagc ggttcccatt tgagctggtg aagatggagt 1201 ttgacgagaa ggacttacga cgggagatca gctatgccat taagaacatc catggagtca 1261 ggaccgggct tttcaccccg gacttggcat tcgaggccat tgtgaaaaag caggtcgtca 1321 agctgaaaga gccctgtctg aaatgtgtcg acctggttat ccaggagcta atcaatacag 1381 ttaggcagtg taccagtaag ctcagttcct acccccggtt gcgagaggag acagagcgaa 1441 tcgtcaccac ttacatccgg gaacgggagg ggagaacgaa ggaccagatt cttctgctga 1501 tcgacattga gcagtcctac atcaacacga accatgagga cttcatcggg tttgccaatg 1561 cccagcagag gagcacgcag ctgaacaaga agagagccat ccccaatcag ggggagatcc 1621 tggtgatccg caggggctgg ctgaccatca acaacatcag cctgatgaaa ggcggctcca 1681 aggagtactg gtttgtgctg actgccgagt cactgtcctg gtacaaggat gaggaggaga 1741 aagagaagaa gtacatgctg cctctggaca acctcaagat ccgtgatgtg gagaagggct 1801 tcatgtccaa caagcacgtc ttcgccatct tcaacacgga gcagagaaac gtctacaagg 1861 acctgcggca gatcgagctg gcctgtgact cccaggaaga cgtggacagc tggaaggcct 1921 cgttcctccg agctggcgtc taccccgaga aggaccaggc agaaaacgag gatggggccc 1981 aggagaacac cttctccatg gacccccaac tggagcggca ggtggagacc attcgcaacc 2041 tggtggactc atacgtggcc atcatcaaca agtccatccg cgacctcatg ccaaagacca 2101 tcatgcacct catgatcaac aatacgaagg ccttcatcca ccacgagctg ctggcctacc 2161 tatactcctc ggcagaccag agcagcctca tggaggagtc ggccgaccag gcacagcggc 2221 gggacgacat gctgcgcatg taccatgccc tcaaggaggc gctcaacatc atcggtgaca 2281 tcagcaccag cactgtgtcc acgcctgtac ccccgcctgt cgatgacacc tggctccaga 2341 gcgccagcag ccacagcccc actccacagc gccgaccggt gtccagcata cacccccctg 2401 gccggccccc agcagtgagg ggccccactc cagggccccc cctgattcct gttcccgtgg 2461 gggcagcagc ctccttctcg gcgcccccaa tcccatcccg gcctggaccc cagagcgtgt 2521 ttgccaacag tgacctcttc ccagccccgc ctcagatccc atctcggcca gttcggatcc 2581 ccccagggat tcccccagga gtgcccagca gaagaccccc tgctgcgccc agccggccca 2641 ccattatccg cccagccgag ccatccctgc tcgactaccc aactttcttg tacaaagtgg 2701 ttggtaagcc tatccctaac cctctcctcg gtctcgattc tacgtagtaa tgagctagcg 2761 ctaaccggtg gcgcgttaag tcgacaatca acctctggat ta