Transcript: Mouse NM_001253893.1

Mus musculus dynamin 2 (Dnm2), transcript variant 1, mRNA.

Source:
NCBI, updated 2017-05-28
Taxon:
Mus musculus (mouse)
Gene:
Dnm2 (13430)
Length:
3582
CDS:
189..2801

Additional Resources:

NCBI RefSeq record:
NM_001253893.1
NBCI Gene record:
Dnm2 (13430)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_001253893.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000349997 GAGCTCCTTTGGCCATATTAA pLKO_005 3224 3UTR 100% 15.000 21.000 N Dnm2 n/a
2 TRCN0000089961 CGCATCAATCGTATCTTTCAT pLKO.1 1269 CDS 100% 5.625 7.875 N Dnm2 n/a
3 TRCN0000089959 GCCCGCATCAATCGTATCTTT pLKO.1 1266 CDS 100% 5.625 7.875 N Dnm2 n/a
4 TRCN0000317550 GCCCGCATCAATCGTATCTTT pLKO_005 1266 CDS 100% 5.625 7.875 N Dnm2 n/a
5 TRCN0000089962 GCAGTCGTACATCAACACAAA pLKO.1 1634 CDS 100% 4.950 6.930 N Dnm2 n/a
6 TRCN0000317551 GCAGTCGTACATCAACACAAA pLKO_005 1634 CDS 100% 4.950 6.930 N Dnm2 n/a
7 TRCN0000089960 GCCCTTGAGAAGAGGCTATAT pLKO.1 863 CDS 100% 13.200 10.560 N Dnm2 n/a
8 TRCN0000317474 GCCCTTGAGAAGAGGCTATAT pLKO_005 863 CDS 100% 13.200 10.560 N Dnm2 n/a
9 TRCN0000089958 CCTAGTGGACATGACAATGAA pLKO.1 2939 3UTR 100% 5.625 3.938 N Dnm2 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001253893.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_06113 pDONR223 100% 89.3% 98.1% None (many diffs) n/a
2 ccsbBroad304_06113 pLX_304 0% 89.3% 98.1% V5 (many diffs) n/a
3 TRCN0000477226 CCTTTCAAGCTCTGCTGCCATAGT pLX_317 16.5% 89.3% 98.1% V5 (many diffs) n/a
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