Construct: ORF ccsbBroad304_06178
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006095.1_s304c1, BRDN0000403782
- Derived from:
- ccsbBroadEn_06178
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ERCC5 (2073)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_06178
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2073 | ERCC5 | ERCC excision repair 5, end... | NM_000123.3 | 99.9% | 100% | 138T>C |
| 2 | human | 100533467 | BIVM-ERCC5 | BIVM-ERCC5 readthrough | NM_001204425.1 | 71.3% | 69.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 3624
- ORF length:
- 3558
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatggg ggtccagggg ctctggaagc tgctggagtg ctccgggcgg caggtcagcc 121 ccgaagcgct ggaagggaag atcctggctg ttgatattag catttggtta aaccaagcac 181 ttaaaggagt ccgggatcgc cacgggaact caatagaaaa tcctcatctt ctcactttgt 241 ttcatcggct ctgcaaactc ttattttttc gaattcgtcc tatttttgtg tttgatgggg 301 atgctccact attgaagaaa cagactttgg tgaagagaag gcagagaaag gacttagcgt 361 ccagtgactc caggaaaacg acagagaagc ttctgaaaac atttttgaaa agacaagcca 421 tcaaaactgc cttcagaagc aaaagagatg aagcactacc cagtcttacc caagttcgaa 481 gagaaaacga cctctatgtt ttgcctcctt tacaagagga agaaaaacac agttcagaag 541 aggaagatga aaaagaatgg caagaaagaa tgaatcaaaa acaagcatta caggaagagt 601 tctttcataa tcctcaagcg atagatattg agtctgagga cttcagcagc ctgccccctg 661 aagtaaagca tgaaatcttg actgatatga aagagttcac caagcgcaga agaacattat 721 ttgaagcaat gccagaggag tctgatgact tttcacagta ccaactcaaa ggcttgctta 781 aaaagaacta tctgaaccag catatagaac atgtccaaaa ggaaatgaat cagcaacatt 841 caggacacat ccgaaggcag tatgaagatg aagggggctt tctgaaggag gtagagtcaa 901 ggagagtggt ctctgaagac acttcacatt acatcttgat aaaaggtatt caagctaaga 961 cagttgcaga agtggattca gagtctcttc cttcttccag caaaatgcac ggcatgtctt 1021 ttgacgtgaa gtcatctcca tgtgaaaaac tgaagacaga gaaagagcct gatgctaccc 1081 ctccttctcc aagaacttta ctagctatgc aagctgccct gctgggaagt agctcagaag 1141 aggagctgga gagtgaaaat cgaaggcagg cccgtgggag gaacgcacct gctgctgtag 1201 acgaaggctc catatcaccc cggactcttt cagccattaa gagagctctt gacgatgacg 1261 aagatgtaaa agtgtgtgct ggggatgatg tgcagacggg agggccagga gcagaagaaa 1321 tgcgtataaa cagctccacc gagaacagtg atgaaggact taaagtgaga gatggaaaag 1381 gaataccgtt tactgcaaca cttgcgtcat ctagtgtgaa ctctgcagag gagcacgtag 1441 ccagcactaa tgaggggaga gagcccacag actcagttcc aaaagaacaa atgtcacttg 1501 ttcacgtggg gactgaagcc tttccgataa gtgatgagtc tatgattaag gacagaaaag 1561 atcggctgcc tctggagagt gcagtggtta gacatagtga cgcacctggg ctcccgaatg 1621 gaagggaact gacaccggca tctccaactt gtacaaattc tgtgtcaaag aatgaaacac 1681 atgctgaagt gcttgagcag cagaacgaac tttgcccata tgagagtaaa ttcgattctt 1741 ctcttctttc aagtgatgat gaaacaaaat gtaaaccgaa ttctgcttct gaagtcattg 1801 gccctgtcag tttgcaagaa acaagtagca tagtaagtgt cccttcagag gcagtagata 1861 atgtggaaaa tgtggtgtca tttaatgcta aagagcatga gaattttctg gaaaccatcc 1921 aagaacagca gaccactgaa tctgcaggcc aggatttaat ttccattcca aaggccgtgg 1981 aaccaatgga aattgactcg gaagaaagtg aatctgatgg aagtttcatt gaagtgcaaa 2041 gtgtgattag tgatgaggaa cttcaagcag aattccctga aacttccaaa cctccctcag 2101 aacaaggcga agaggaactg gtaggaacta gggagggaga agcccctgct gagtccgaga 2161 gcctcctgag ggacaactct gagagggacg acgtggatgg tgagccacag gaagctgaga 2221 aagatgcgga agattcgctc catgaatggc aagatattaa tttggaggag ttggaaactc 2281 tggagagcaa cctcttagca cagcagaatt cactgaaagc tcaaaaacag cagcaagaac 2341 ggatcgctgc tactgtcacc ggacagatgt tcctggaaag ccaggaactc ctgcgcctgt 2401 tcggcattcc ctacatccag gctcccatgg aagcagaggc gcagtgcgcc atcctggacc 2461 tgactgatca gacttccgga accatcactg atgacagtga tatctggctg tttggagcgc 2521 ggcatgtcta tagaaacttt tttaataaaa acaagtttgt agaatattat caatatgtgg 2581 actttcacaa tcaattggga ttggaccgga ataagttaat aaatttggct tatttgcttg 2641 gaagtgatta taccgaagga ataccaactg tgggttgtgt aaccgccatg gaaattctca 2701 atgaattccc tgggcatggc ctggaacctc tcctaaaatt ctcagaatgg tggcatgaag 2761 ctcaaaaaaa tccaaagata agacctaatc ctcatgacac caaagtgaaa aaaaaattac 2821 ggacattgca actcacccct ggctttccta acccagctgt tgccgaggcc tacctcaaac 2881 ccgtggtgga tgactcgaag ggatcctttc tgtgggggaa acctgatctc gacaaaatta 2941 gagaattttg tcagcggtat ttcggctgga acagaacgaa gacagatgaa tctctgtttc 3001 ctgtattaaa gcaactcgat gcccagcaga cacagctccg aattgattcc ttctttagat 3061 tagcacaaca ggagaaagaa gatgctaaac gtattaagag ccagagacta aacagagctg 3121 tgacatgtat gctaaggaaa gagaaagaag cagcagccag cgaaatagaa gcagtttctg 3181 ttgccatgga gaaagaattt gagctacttg ataaggcaaa acgaaaaacc cagaagagag 3241 gcataacaaa taccttagaa gagtcatcaa gcctgaaaag aaagaggctt tcagattcta 3301 aacgaaagaa tacatgcggt ggatttttgg gggagacctg cctctcagaa tcatctgatg 3361 gatcttcaag tgaagatgct gaaagttcat ctttaatgaa tgtacaaagg agaacagctg 3421 cgaaagagcc aaaaaccagt gcttcagatt cgcagaactc agtgaaggaa gctcccgtga 3481 agaatggagg tgcgaccacc agcagctcta gtgatagtga tgacgatgga gggaaagaga 3541 agatggtcct cgtgaccgcc agatctgtgt ttgggaagaa aagaaggaaa ctaagacgtg 3601 cgaggggaag aaaaaggaaa acctacccaa ctttcttgta caaagtggtt ggtaagccta 3661 tccctaaccc tctcctcggt ctcgattcta cgtagtaatg agctagcgct aaccggtggc 3721 gcgttaagtc gacaatcaac ctctggatta