Transcript: Human NM_001204425.1

Homo sapiens BIVM-ERCC5 readthrough (BIVM-ERCC5), mRNA.

Source:
NCBI, updated 2018-05-12
Taxon:
Homo sapiens (human)
Gene:
BIVM-ERCC5 (100533467)
Length:
5143
CDS:
123..5045

Additional Resources:

NCBI RefSeq record:
NM_001204425.1
NBCI Gene record:
BIVM-ERCC5 (100533467)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001204425.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000358809 AGAAGATGCTAAACGTATTAA pLKO_005 4496 CDS 100% 15.000 7.500 Y ERCC5 n/a
2 TRCN0000358807 AGCAGAAGAAATGCGTATAAA pLKO_005 2729 CDS 100% 15.000 7.500 Y ERCC5 n/a
3 TRCN0000358878 TTGGGATTGGACCGGAATAAG pLKO_005 4014 CDS 100% 13.200 6.600 Y ERCC5 n/a
4 TRCN0000358806 CCGATAAGTGATGAGTCTATG pLKO_005 2943 CDS 100% 10.800 5.400 Y ERCC5 n/a
5 TRCN0000435725 GATATTGTTACTGATCTAAAC pLKO_005 1251 CDS 100% 10.800 5.400 Y BIVM n/a
6 TRCN0000130597 CCCGTGGATTGAAAGATGAAT pLKO.1 1048 CDS 100% 5.625 2.813 Y BIVM n/a
7 TRCN0000146354 CCACAGTATAAGACTTCTTGT pLKO.1 744 CDS 100% 4.950 2.475 Y BIVM n/a
8 TRCN0000050778 CCAGCGAAATAGAAGCAGTTT pLKO.1 4576 CDS 100% 4.950 2.475 Y ERCC5 n/a
9 TRCN0000050779 CCTCCTTTACAAGAGGAAGAA pLKO.1 1923 CDS 100% 4.950 2.475 Y ERCC5 n/a
10 TRCN0000050780 GCTTTCAGATTCTAAACGAAA pLKO.1 4706 CDS 100% 4.950 2.475 Y ERCC5 n/a
11 TRCN0000130467 GCTTTACATATTCTGGGCTTT pLKO.1 849 CDS 100% 4.050 2.025 Y BIVM n/a
12 TRCN0000050782 CCAATGGAAATTGACTCGGAA pLKO.1 3402 CDS 100% 2.640 1.320 Y ERCC5 n/a
13 TRCN0000050781 CCTGTATTAAAGCAACTCGAT pLKO.1 4419 CDS 100% 2.640 1.320 Y ERCC5 n/a
14 TRCN0000175161 GCTTATATCTATCATTGCCAA pLKO.1 1074 CDS 100% 2.640 1.320 Y Bivm n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001204425.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_06178 pDONR223 100% 71.3% 69.8% None (many diffs) n/a
2 ccsbBroad304_06178 pLX_304 0% 71.3% 69.8% V5 (many diffs) n/a
3 TRCN0000478964 ACTTTTTCTGAGTATACGTCTAAT pLX_317 9.6% 71.3% 69.8% V5 (many diffs) n/a
4 ccsbBroadEn_03466 pDONR223 100% 30.1% 29.8% None (many diffs) n/a
5 ccsbBroad304_03466 pLX_304 0% 30.1% 29.8% V5 (many diffs) n/a
6 TRCN0000478072 GAGTGCCCCGGCTGTTTGGCGAGC pLX_317 23.6% 30.1% 29.8% V5 (many diffs) n/a
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