Construct: ORF ccsbBroad304_06223

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF014674.1_s304c1, BRDN0000399559
Derived from:
ccsbBroadEn_06223
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
FRZB (2487)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_06223
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 2487 FRZB frizzled related protein NM_001463.4 99.8% 99.6% 975C>A
2 mouse 20378 Frzb frizzled-related protein NM_011356.4 89.1% 91.3% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1041
ORF length:
975
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatggt ctgcggcagc ccgggaggga tgctgctgct gcgggccggg ctgcttgccc
121tggctgctct ctgcctgctc cgggtgcccg gggctcgggc tgcagcctgt gagcccgtcc
181gcatccccct gtgcaagtcc ctgccctgga acatgactaa gatgcccaac cacctgcacc
241acagcactca ggccaacgcc atcctggcca tcgagcagtt cgaaggtctg ctgggcaccc
301actgcagccc cgatctgctc ttcttcctct gtgccatgta cgcgcccatc tgcaccattg
361acttccagca cgagcccatc aagccctgta agtctgtgtg cgagcgggcc cggcagggct
421gtgagcccat actcatcaag taccgccact cgtggccgga gaacctggcc tgcgaggagc
481tgccagtgta cgacaggggc gtgtgcatct ctcccgaggc catcgttact gcggacggag
541ctgattttcc tatggattct agtaacggaa actgtagagg ggcaagcagt gaacgctgta
601aatgtaagcc tattagagct acacagaaga cctatttccg gaacaattac aactatgtca
661ttcgggctaa agttaaagag ataaagacta agtgccatga tgtgactgca gtagtggagg
721tgaaggagat tctaaagtcc tctctggtaa acattccacg ggacactgtc aacctctata
781ccagctctgg ctgcctctgc cctccactta atgttaatga ggaatatatc atcatgggct
841atgaagatga ggaacgttcc agattactct tggtggaagg ctctatagct gagaagtgga
901aggatcgact cggtaaaaaa gttaagcgct gggatatgaa gcttcgtcat cttggactca
961gtaaaagtga ttctagcaat agtgattcca ctcagagtca gaagtctggc aggaactcga
1021acccccggca agcacgcaaa tacccaactt tcttgtacaa agtggttggt aagcctatcc
1081ctaaccctct cctcggtctc gattctacgt agtaatgagc tagcgctaac cggtggcgcg
1141ttaagtcgac aatcaacctc tggatta

Download FASTA (ORF) (Full)