Construct: ORF ccsbBroad304_06224

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF011398.1_s304c1, BRDN0000408777
Derived from:
ccsbBroadEn_06224
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
FSHR (2492)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_06224
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 2492 FSHR follicle stimulating hormon... NM_000145.4 99.9% 99.7% 919G>A;2039G>A
2 human 2492 FSHR follicle stimulating hormon... NM_181446.3 96.1% 95.9% 444_445ins78;841G>A;1961G>A
3 human 2492 FSHR follicle stimulating hormon... XM_011532733.2 95.2% 95% 668_769del;1021G>A;2141G>A
4 human 2492 FSHR follicle stimulating hormon... XM_011532734.2 78.7% 77.4% (many diffs)
5 human 2492 FSHR follicle stimulating hormon... XM_011532735.2 61.9% 61.7% 0_1ins792;127G>A;1247G>A
6 human 2492 FSHR follicle stimulating hormon... XM_011532736.2 61.9% 61.7% 0_1ins792;127G>A;1247G>A
7 human 2492 FSHR follicle stimulating hormon... XM_011532740.1 39% 39% 668_769del;957_957delAins1231
8 mouse 14309 Fshr follicle stimulating hormon... NM_013523.3 86.3% 87.6% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
2154
ORF length:
2085
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcaccat ggccctgctc ctggtctctt tgctggcatt cctgagcttg ggctcaggat
121gtcatcatcg gatctgtcac tgctctaaca gggtttttct ctgccaagag agcaaggtga
181cagagattcc ttctgacctc ccgaggaatg ccattgaact gaggtttgtc ctcaccaagc
241ttcgagtcat ccaaaaaggt gcattttcag gatttgggga cctggagaaa atagagatct
301ctcagaatga tgtcttggag gtgatagagg cagatgtgtt ctccaacctt cccaaattac
361atgaaattag aattgaaaag gccaacaacc tgctctacat caaccctgag gccttccaga
421accttcccaa ccttcaatat ctgttaatat ccaacacagg tattaagcac cttccagatg
481ttcacaagat tcattctctc caaaaagttt tacttgacat tcaagataac ataaacatcc
541acacaattga aagaaattct ttcgtggggc tgagctttga aagtgtgatt ctatggctga
601ataagaatgg gattcaagaa atacacaact gtgcattcaa tggaacccaa ctagatgagc
661tgaatctaag cgataataat aatttagaag aattgcctaa tgatgttttc cacggagcct
721ctggaccagt cattctagat atttcaagaa caaggatcca ttccctgcct agctatggct
781tagaaaatct taagaagctg agggccaggt cgacttacaa cttaaaaaag ctgcctactc
841tggaaaagct tgtcgccctc atggaagcca gcctcaccta tcccagccat tgctgtgcct
901ttgcaaactg gagacggcaa atctctgagc ttcatccaat ttgcaacaaa tctattttaa
961ggcaagaagt tgattatatg actcagacta ggggtcagag atcctctctg gcagaagaca
1021atgagtccag ctacagcaga ggatttgaca tgacgtacac tgagtttgac tatgacttat
1081gcaatgaagt ggttgacgtg acctgctccc ctaagccaga tgcattcaac ccatgtgaag
1141atatcatggg gtacaacatc ctcagagtcc tgatatggtt tatcagcatc ctggccatca
1201ctgggaacat catagtgcta gtgatcctaa ctaccagcca atataaactc acagtcccca
1261ggttccttat gtgcaacctg gcctttgctg atctctgcat tggaatctac ctgctgctca
1321ttgcatcagt tgatatccat accaagagcc aatatcacaa ctatgccatt gactggcaaa
1381ctggggcagg ctgtgatgct gctggctttt tcactgtctt tgccagtgag ctgtcagtct
1441acactctgac agctatcacc ttggaaagat ggcataccat cacgcatgcc atgcagctgg
1501actgcaaggt gcagctccgc catgctgcca gtgtcatggt gatgggctgg atttttgctt
1561ttgcagctgc cctctttccc atctttggca tcagcagcta catgaaggtg agcatctgcc
1621tgcccatgga tattgacagc cctttgtcac agctgtatgt catgtccctc cttgtgctca
1681atgtcctggc ctttgtggtc atctgtggct gctatatcca catctacctc acagtgcgga
1741accccaacat cgtgtcctcc tctagtgaca ccaggatcgc caagcgcatg gccatgctca
1801tcttcactga cttcctctgc atggcaccca tttctttctt tgccatttct gcctccctca
1861aggtgcccct catcactgtg tccaaagcaa agattctgct ggttctgttt caccccatca
1921actcctgtgc caaccccttc ctctatgcca tctttaccaa aaactttcgc agagatttct
1981tcattctgct gagcaagtgt ggctgctatg aaatgcaagc ccaaatttat aggacagaaa
2041cttcatccac tgtccacaac acccatccaa ggaatggcca ctgctcttca gctcccagag
2101tcaccaatgg ttccacttac atacttgtcc ctctaagtca tttagcccaa aacttgccaa
2161ctttcttgta caaagtggtt ggtaagccta tccctaaccc tctcctcggt ctcgattcta
2221cgtagtaatg agctagcgct aaccggtggc gcgttaagtc gacaatcaac ctctggatta

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