Construct: ORF ccsbBroad304_06253
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001704.1_s304c1, BRDN0000402337
- Derived from:
- ccsbBroadEn_06253
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GALT (2592)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_06253
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2592 | GALT | galactose-1-phosphate uridy... | NM_000155.4 | 99.9% | 99.7% | 940A>G |
| 2 | human | 2592 | GALT | galactose-1-phosphate uridy... | NM_001258332.1 | 71.1% | 65% | 0_1ins202;49_50ins125;613A>G |
| 3 | mouse | 14430 | Galt | galactose-1-phosphate uridy... | NM_016658.3 | 82.5% | 87% | (many diffs) |
| 4 | mouse | 14430 | Galt | galactose-1-phosphate uridy... | NM_001302511.1 | 46% | 48.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1203
- ORF length:
- 1137
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgtc gcgcagtgga accgatcctc agcaacgcca gcaggcgtca gaggcggacg 121 ccgcagcagc aaccttccgg gcaaacgacc atcagcatat ccgctacaac ccgctgcagg 181 atgagtgggt gctggtgtca gctcaccgca tgaagcggcc ctggcagggt caagtggagc 241 cccagcttct gaagacagtg ccccgccatg accctctcaa ccctctgtgt cctggggcca 301 tccgagccaa cggagaggtg aatccccagt acgatagcac cttcctgttt gacaacgact 361 tcccagctct gcagcctgat gcccccagtc caggacccag tgatcatccc cttttccaag 421 caaagtctgc tcgaggagtc tgtaaggtca tgtgcttcca cccctggtcg gatgtaacgc 481 tgccactcat gtcggtccct gagatccggg ctgttgttga tgcatgggcc tcagtcacag 541 aggagctggg tgcccagtac ccttgggtgc agatctttga aaacaaaggt gccatgatgg 601 gctgttctaa cccccacccc cactgccagg tatgggccag cagtttcctg ccagatattg 661 cccagcgtga ggagcgatct cagcaggcct ataagagtca gcatggagag cccctgctaa 721 tggagtacag ccgccaggag ctactcagga aggaacgtct ggtcctaacc agtgagcact 781 ggttagtact ggtccccttc tgggcaacat ggccctacca gacactgctg ctgccccgtc 841 ggcatgtgcg gcggctacct gagctgaccc ctgctgagcg tgatgatcta gcctccatca 901 tgaagaagct cttgaccaag tatgacaacc tctttgagac gtcctttccc tactccatgg 961 gctggcatgg ggctcccaca ggatcagagg ctggggccaa ctgggaccat tggcagctgc 1021 acgctcatta ctaccctccg ctcctgcgct ctgccactgt ccggaaattc atggttggct 1081 acgaaatgct tgctcaggct cagagggacc tcacccctga gcaggctgca gagagactaa 1141 gggcacttcc tgaggttcat taccacctgg ggcagaagga cagggagaca gcaaccatcg 1201 cctgcccaac tttcttgtac aaagtggttg gtaagcctat ccctaaccct ctcctcggtc 1261 tcgattctac gtagtaatga gctagcgcta accggtggcg cgttaagtcg acaatcaacc 1321 tctggatta