Construct: ORF ccsbBroad304_06262
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016734.1_s304c1, BRDN0000406934
- Derived from:
- ccsbBroadEn_06262
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GBE1 (2632)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_06262
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2632 | GBE1 | 1,4-alpha-glucan branching ... | NM_000158.4 | 99.9% | 99.7% | 793A>T;1000A>G |
| 2 | mouse | 74185 | Gbe1 | glucan (1,4-alpha-), branch... | NM_028803.4 | 86.6% | 91.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2172
- ORF length:
- 2106
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatggc ggctccgatg actcccgcgg ctcggcccga ggactacgag gcggcgctca 121 atgccgccct ggctgacgtg cccgaactgg ccagactcct ggagatcgac ccgtacttga 181 agccctacgc cgtggacttc cagcgcaggt ataagcagtt tagccaaatt ttgaagaaca 241 ttggagaaaa tgaaggtggt attgataagt tttccagagg ctatgaatca tttggcgtcc 301 acagatgtgc tgatggtggt ttatactgca aagaatgggc cccgggagca gaaggagttt 361 ttcttactgg agattttaat ggttggaatc cattttcgta cccatacaaa aaactggatt 421 atggaaaatg ggagctgtat atcccaccaa agcagaataa atctgtactc gtgcctcatg 481 gatccaaatt aaaggtagtt attactagta aaagcggaga gatcttgtat cgtatttcac 541 cgtgggcaaa gtatgtggtt cgtgaaggtg ataatgtgaa ttatgattgg atacactggg 601 atccagaaca ctcatatgag tttaagcatt ccagaccaaa gaagccacgg agtctaagaa 661 tttatgaatc tcatgtggga atttcttccc atgaaggaaa agtagcttct tataaacatt 721 ttacatgcaa tgtactacca agaatcaaag gccttggata caactgcatt cagttgatgg 781 caatcatgga gcatgcttac tatgccagct ttggttacca aatcacaagc ttctttgcag 841 cttccagccg ttatggatca cctgaagagc tacaagaact ggtagacaca gctcattcca 901 tgggtatcat agtcctctta gatgtggtac acagccatgc ttcaaaaaat tcagcagatg 961 gattgaatat gtttgatggg acagattcct gttattttca ttctggacct agagggactc 1021 atgatctttg ggatagcaga ttgtttgcct actccagctg ggaagtttta agattccttc 1081 tgtcaaacat aagatggtgg ttggaagaat atcgctttga tggatttcgt tttgatggtg 1141 ttacgtccat gctttatcat caccatggag tgggtcaagg tttctcaggt gattacagtg 1201 aatatttcgg actacaagta gatgaagatg ccttgactta cctcatgttg gcaaatcatt 1261 tggttcacac gctgtgtccc gattctataa caatagctga ggatgtatca ggaatgccag 1321 ctctgtgctc tccaatttcc cagggagggg gtggttttga ctatcgacta gccatggcaa 1381 ttccagataa gtggattcag ctacttaaag agtttaaaga tgaagactgg aacatgggcg 1441 atatagtata cacgctcaca aacaggcgct accttgaaaa gtgcattgct tatgcagaga 1501 gccatgatca ggcattggtt ggggataagt cgctggcatt ttggttgatg gatgccgaaa 1561 tgtatacaaa catgagtgtc ctgactcctt ttactccagt tattgatcgt ggaatacagc 1621 ttcataaaat gattcgactc attacgcatg ggcttggtgg agaaggctat ctcaatttca 1681 tgggtaatga atttgggcat cctgaatggt tagacttccc aagaaaagga aataatgaga 1741 gttaccatta tgccaggcgg cagtttcatt taactgacga cgaccttctt cgctacaagt 1801 tcctaaataa ttttgacagg gatatgaata gattggaaga aagatatggt tggcttgcag 1861 ctccacaggc ctacgtgagt gaaaaacatg aaggcaataa gatcattgct tttgaaagag 1921 caggtcttct tttcattttc aacttccatc caagcaagag ctacactgac taccgagttg 1981 gaacagcatt gccagggaaa ttcaaaattg tgctagattc agatgcagcg gaatatggag 2041 ggcatcagag actggaccac agcactgact ttttttctga ggcttttgaa cataatgggc 2101 gtccctattc tcttttggtg tacattccaa gcagagtggc cctcatcctt cagaatgtgg 2161 atctgccgaa ttgcccaact ttcttgtaca aagtggttgg taagcctatc cctaaccctc 2221 tcctcggtct cgattctacg tagtaatgag ctagcgctaa ccggtggcgc gttaagtcga 2281 caatcaacct ctggatta