Construct: ORF ccsbBroad304_06363
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007907.1_s304c1, BRDN0000461955
- Derived from:
- ccsbBroadEn_06363
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- HGFAC (3083)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_06363
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3083 | HGFAC | HGF activator | NM_001528.4 | 99.8% | 99.8% | 618C>A;693C>G |
| 2 | human | 3083 | HGFAC | HGF activator | NM_001297439.2 | 98.8% | 98.7% | 618C>A;693C>G;1102_1122del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2034
- ORF length:
- 1965
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat ggggcgctgg gcctgggtcc ccagcccctg gcccccaccg gggctgggcc 121 ccttcctcct cctcctcctg ctgctgctgc tgctgccacg ggggttccag ccccagcctg 181 gcgggaaccg tacggagtcc ccagaaccta atgccacagc gacccctgcg atccccacta 241 tcctggtgac ctctgtgacc tctgagaccc cagcaacaag tgctccagag gcagagggac 301 cccaaagtgg ggggctcccg cccccgccca gggcagttcc ctcgagcagt agcccccagg 361 cccaagcact caccgaggac gggaggccct gcaggttccc cttccgctac gggggccgca 421 tgctgcatgc ctgcacttcg gagggcagtg cacacaggaa gtggtgtgcc acaactcaca 481 actacgaccg ggacagggcc tggggctact gtgtggaggc caccccgcct ccagggggcc 541 cagctgccct ggatccctgt gcctccggcc cctgcctcaa tggaggctcc tgctccaata 601 cccaggaccc ccagtcctat cactgcagct gcccccgggc cttcaccggc aaggactgcg 661 gcacagagaa atgctttgat gagacacgct acgagtacct ggaggggggc gaccgctggg 721 cccgcgtgcg ccagggccac gtggaacagt gcgagtgctt ggggggccgg acctggtgcg 781 aaggcacccg acatacagct tgtctgagca gcccttgcct gaacgggggc acctgccacc 841 tgatcgtggc caccgggacc accgtgtgtg cctgcccacc aggcttcgct ggacggctct 901 gcaacatcga gcctgatgag cgctgcttct tggggaacgg cactgggtac cgtggcgtgg 961 ccagcacctc agcctcgggc ctcagctgcc tggcctggaa ctccgatctg ctctaccagg 1021 agctgcacgt ggactccgtg ggcgccgcgg ccctgctggg cctgggcccc catgcctact 1081 gccggaatcc ggacaatgac gagaggccct ggtgctacgt ggtgaaggac agcgcgctct 1141 cctgggagta ctgccgcctg gaggcctgcg aatccctcac cagagtccaa ctgtcaccgg 1201 atctcctggc gaccctgcct gagccagcct ccccggggcg ccaggcctgc ggcaggaggc 1261 acaagaagag gacgttcctg cggccacgta tcatcggcgg ctcctcctcg ctgcccggct 1321 cgcacccctg gctggccgcc atctacatcg gggacagctt ctgcgccggg agcctggtcc 1381 acacctgctg ggtggtgtcg gccgcccact gcttctccca cagccccccc agggacagcg 1441 tctccgtggt gctgggccag cacttcttca accgcacgac ggacgtgacg cagaccttcg 1501 gcatcgagaa gtacatcccg tacaccctgt actcggtgtt caaccccagc gaccacgacc 1561 tcgtcctgat ccggctgaag aagaaagggg accgctgtgc cacacgctcg cagttcgtgc 1621 agcccatctg cctgcccgag cccggcagca ccttccccgc aggacacaag tgccagattg 1681 cgggctgggg ccacttggat gagaacgtga gcggctactc cagctccctg cgggaggccc 1741 tggtccccct ggtcgccgac cacaagtgca gcagccctga ggtctacggc gccgacatca 1801 gccccaacat gctctgtgcc ggctacttcg actgcaagtc cgacgcctgc cagggggact 1861 caggggggcc cctggcctgc gagaagaacg gcgtggctta cctctacggc atcatcagct 1921 ggggtgacgg ctgcgggcgg ctccacaagc cgggggtcta cacccgcgtg gccaactatg 1981 tggactggat caacgaccgg atacggcctc ccaggcggct tgtggctccc tccttgccaa 2041 ctttcttgta caaagtggtt ggtaagccta tccctaaccc tctcctcggt ctcgattcta 2101 cgtagtaatg agctagcgct aaccggtggc gcgttaagtc gacaatcaac ctctggatta