Construct: ORF ccsbBroad304_06367

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF016685.1_s304c1, BRDN0000399200
Derived from:
ccsbBroadEn_06367
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
HK1 (3098)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_06367
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 3098 HK1 hexokinase 1 NM_000188.2 99.9% 100% 78C>G;1443G>A
2 human 3098 HK1 hexokinase 1 NM_033496.2 98.3% 96% (many diffs)
3 human 3098 HK1 hexokinase 1 NM_001322364.1 98.1% 96.7% (many diffs)
4 human 3098 HK1 hexokinase 1 NM_001358263.1 98.1% 96.7% (many diffs)
5 human 3098 HK1 hexokinase 1 NM_033497.2 98.1% 96.7% (many diffs)
6 human 3098 HK1 hexokinase 1 NM_033498.2 98.1% 96.7% (many diffs)
7 human 3098 HK1 hexokinase 1 XM_024447969.1 98.1% 96.7% (many diffs)
8 human 3098 HK1 hexokinase 1 NM_033500.2 97.3% 97.2% (many diffs)
9 human 3098 HK1 hexokinase 1 XM_011539732.1 97.3% 97.2% (many diffs)
10 human 3098 HK1 hexokinase 1 NM_001322366.1 96.9% 96.9% 0_1ins84;1359G>A
11 human 3098 HK1 hexokinase 1 XM_005269737.1 96.9% 96.9% 0_1ins84;1359G>A
12 human 3098 HK1 hexokinase 1 NM_001322367.1 96.4% 96.5% 78C>G;494_495ins96;1347G>A
13 human 3098 HK1 hexokinase 1 NM_001322365.1 95.1% 94.4% (many diffs)
14 mouse 15275 Hk1 hexokinase 1 NM_001146100.1 86.7% 92.7% (many diffs)
15 mouse 15275 Hk1 hexokinase 1 XM_006513246.3 85.5% 89.2% (many diffs)
16 mouse 15275 Hk1 hexokinase 1 XM_006513247.1 85.2% 90.6% (many diffs)
17 mouse 15275 Hk1 hexokinase 1 XM_006513243.3 84.7% 89.4% (many diffs)
18 mouse 15275 Hk1 hexokinase 1 XM_006513244.3 84.7% 89.4% (many diffs)
19 mouse 15275 Hk1 hexokinase 1 XM_006513245.3 84.7% 89.4% (many diffs)
20 mouse 15275 Hk1 hexokinase 1 NM_010438.3 82.7% 87.3% (many diffs)
21 mouse 15275 Hk1 hexokinase 1 XM_006513242.1 81.2% 84.7% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
2817
ORF length:
2751
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatgat cgccgcgcag ctcctggcct attacttcac ggagctgaag gatgaccagg
121tcaaaaagat tgacaagtat ctgtatgcca tgcggctctc cgatgaaact ctcatagata
181tcatgactcg cttcaggaag gagatgaaga atggcctctc ccgggatttt aatccaacag
241ccacagtcaa gatgttgcca acattcgtaa ggtccattcc tgatggctct gaaaagggag
301atttcattgc cctggatctt ggtgggtctt cctttcgaat tctgcgggtg caagtgaatc
361atgagaaaaa ccagaatgtt cacatggagt ccgaggttta tgacacccca gagaacatcg
421tgcacggcag tggaagccag ctttttgatc atgttgctga gtgcctggga gatttcatgg
481agaaaaggaa gatcaaggac aagaagttac ctgtgggatt cacgttttct tttccttgcc
541aacaatccaa aatagatgag gccatcctga tcacctggac aaagcgattt aaagcgagcg
601gagtggaagg agcagatgtg gtcaaactgc ttaacaaagc catcaaaaag cgaggggact
661atgatgccaa catcgtagct gtggtgaatg acacagtggg caccatgatg acctgtggct
721atgacgacca gcactgtgaa gtcggcctga tcatcggcac tggcaccaat gcttgctaca
781tggaggaact gaggcacatt gatctggtgg aaggagacga ggggaggatg tgtatcaata
841cagaatgggg agcctttgga gacgatggat cattagaaga catccggaca gagtttgaca
901gggagataga ccggggatcc ctcaaccctg gaaaacagct gtttgagaag atggtcagtg
961gcatgtactt gggagagctg gttcgactga tcctagtcaa gatggccaag gagggcctct
1021tatttgaagg gcggatcacc ccggagctgc tcacccgagg gaagtttaac accagtgatg
1081tgtcagccat cgaaaagaat aaggaaggcc tccacaatgc caaagaaatc ctgacccgcc
1141tgggagtgga gccgtccgat gatgactgtg tctcagtcca gcacgtttgc accattgtct
1201catttcgctc agccaacttg gtggctgcca cactgggcgc catcttgaac cgcctgcgtg
1261ataacaaggg cacacccagg ctgcggacca cggttggtgt cgacggatct ctttacaaga
1321cgcacccaca gtattcccgg cgtttccaca agactctaag gcgcttggtg ccagactccg
1381atgtgcgctt cctcctctcg gagagtggca gcggcaaggg ggctgccatg gtgacggcgg
1441tggcctaccg cttggccgag cagcaccggc agatagagga gaccctggct catttccacc
1501tcaccaaaga catgctgctg gaggtgaaga agaggatgcg ggccgagatg gagctggggc
1561tgaggaagca gacgcacaac aatgccgtgg ttaagatgct gccctccttc gtccggagaa
1621ctcccgacgg gaccgagaat ggtgacttct tggccctgga tcttggagga accaatttcc
1681gtgtgctgct ggtgaaaatc cgtagtggga aaaagagaac ggtggaaatg cacaacaaga
1741tctacgccat tcctattgaa atcatgcagg gcactgggga agagctgttt gatcacattg
1801tctcctgcat ctctgacttc ttggactaca tggggatcaa aggccccagg atgcctctgg
1861gcttcacgtt ctcatttccc tgccagcaga cgagtctgga cgcgggaatc ttgatcacgt
1921ggacaaaggg ttttaaggca acagactgcg tgggccacga tgtagtcacc ttactaaggg
1981atgcgataaa aaggagagag gaatttgacc tggacgtggt ggctgtggtc aacgacacag
2041tgggcaccat gatgacctgt gcttatgagg agcccacctg tgaggttgga ctcattgttg
2101ggaccggcag caatgcctgc tacatggagg agatgaagaa cgtggagatg gtggaggggg
2161accaggggca gatgtgcatc aacatggagt ggggggcctt tggggacaac gggtgtctgg
2221atgatatcag gacacactac gacagactgg tggacgaata ttccctaaat gctgggaaac
2281aaaggtatga gaagatgatc agtggtatgt acctgggtga aatcgtccgc aacatcttaa
2341tcgacttcac caagaaggga ttcctcttcc gagggcagat ctctgagacg ctgaagaccc
2401ggggcatctt tgagaccaag tttctctctc agatcgagag tgaccgatta gcactgctcc
2461aggtccgggc tatcctccag cagctaggtc tgaatagcac ctgcgatgac agtatcctcg
2521tcaagacagt gtgcggggtg gtgtccagga gggccgcaca gctgtgtggc gcaggcatgg
2581ctgcggttgt ggataagatc cgcgagaaca gaggactgga ccgtctgaat gtgactgtgg
2641gagtggacgg gacactctac aagcttcatc cacacttctc cagaatcatg caccagacgg
2701tgaaggaact gtcaccaaaa tgtaacgtgt ccttcctcct gtctgaggat ggcagcggca
2761agggggccgc cctcatcacg gccgtgggcg tgcggttacg cacagaggca agcagctacc
2821caactttctt gtacaaagtg gttggtaagc ctatccctaa ccctctcctc ggtctcgatt
2881ctacgtagta atgagctagc gctaaccggt ggcgcgttaa gtcgacaatc aacctctgga
2941tta

Download FASTA (ORF) (Full)