Construct: ORF ccsbBroad304_06400
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013207.1_s304c1, BRDN0000406765
- Derived from:
- ccsbBroadEn_06400
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- AGFG2 (3268)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_06400
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3268 | AGFG2 | ArfGAP with FG repeats 2 | NM_006076.5 | 98.8% | 98.7% | 1046T>C;1429_1443del |
| 2 | human | 3268 | AGFG2 | ArfGAP with FG repeats 2 | XM_005250306.2 | 96.6% | 96.5% | 877_909del;1079T>C;1462_1476del |
| 3 | human | 3268 | AGFG2 | ArfGAP with FG repeats 2 | XM_017012112.1 | 80.8% | 80.6% | 0_1ins261;785T>C;1168_1182del |
| 4 | human | 3268 | AGFG2 | ArfGAP with FG repeats 2 | XM_017012113.1 | 61.6% | 61.3% | 878_879delTC;883delT;885_886ins546 |
| 5 | human | 3268 | AGFG2 | ArfGAP with FG repeats 2 | XM_017012114.1 | 46.7% | 45.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1494
- ORF length:
- 1428
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatggt gatggcggcg aagaagggcc cgggcccggg cggcggggtc agcgggggca 121 aggcggaggc ggaggcggcc tcggaggtgt ggtgccgtcg ggtgcgggag ctgggtggct 181 gcagccaggc cgggaaccgc cactgcttcg agtgcgccca gcgcggggtc acctacgtgg 241 atatcaccgt gggcagcttc gtgtgcacca cctgctccgg cctcctgaga gggctgaacc 301 cccctcatcg tgtcaagtca atctccatga caactttcac tgagcctgaa gtagtattcc 361 tgcaatcccg tggaaatgag gtttgccgga agatttggtt gggtctgttt gatgctcgga 421 catctttagt accagattcc agggatcctc agaaagtgaa ggagtttctc caggaaaaat 481 atgagaagaa gagatggtat gtccccccag accaagtcaa ggggcccact tataccaaag 541 gcagtgcctc cacccctgtg cagggctcca tcccagaagg gaagcccctt cggacacttc 601 tgggtgatcc tgcaccgtct ctctcagttg ctgcctccac ctcgagccag cccgtcagtc 661 agtctcacgc tcggacatcc caggcccgga gcactcagcc acctccccac tcctctgtca 721 aaaaagccag tactgacctg ctggctgaca tcggtggaga cccctttgct gcaccccaga 781 tggcaccagc ttttgctgca ttccctgcct ttgggggcca gacaccttcc caaggaggct 841 ttgccaactt tgatgccttt agcagtggcc ccagctcttc tgtgtttgga agcctccctc 901 cagctggtca agcctcgttc caggcccagc caactcctgc agggagcagc caggggactc 961 catttggtgc cactcccctg gcacccgcca gtcagccaaa cagcctcgca gacgtgggca 1021 gcttcctggg acccggggtg cccgctgcag gtgttcctag cagcctcttc gggatggctg 1081 gccaggtccc cccgctccag tctgtcacga cgggcggcgg cggcggcagc agcacagggc 1141 tggcctttgg agccttcact aaccctttca cagctcccgc cgcccagtcc ccgctgcctt 1201 ccaccaaccc gttccagccc aatggcttgg cgccagggcc cggctttggg atgagcagtg 1261 ctgggcctgg cttcccccag gcagtgccac ccactggggc ctttgccagc tccttcccag 1321 caccgctgtt ccccccgcag accccgcttg ttcagcagca gaatggctct tccttcgggg 1381 acttaggatc agccaagttg gggcagaggc cactgagcca gccagctggg atctccacca 1441 accccttcat gactggaccc tcatcaagcc cattcgcctc caaacctcca accaccaacc 1501 ccttcttgta cccaactttc ttgtacaaag tggttggtaa gcctatccct aaccctctcc 1561 tcggtctcga ttctacgtag taatgagcta gcgctaaccg gtggcgcgtt aagtcgacaa 1621 tcaacctctg gatta