Transcript: Human XM_017012113.1

PREDICTED: Homo sapiens ArfGAP with FG repeats 2 (AGFG2), transcript variant X3, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
AGFG2 (3268)
Length:
1025
CDS:
123..1010

Additional Resources:

NCBI RefSeq record:
XM_017012113.1
NBCI Gene record:
AGFG2 (3268)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_017012113.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000149837 CGGACATCTTTAGTACCAGAT pLKO.1 474 CDS 100% 4.050 5.670 N AGFG2 n/a
2 TRCN0000147372 GTCAATCTCCATGACAACTTT pLKO.1 374 CDS 100% 5.625 3.938 N AGFG2 n/a
3 TRCN0000419486 TGAGAAGAAGAGATGGTATGT pLKO_005 539 CDS 100% 4.950 3.465 N AGFG2 n/a
4 TRCN0000146397 CCAACTTTGATGCCTTTAGCA pLKO.1 901 CDS 100% 3.000 2.100 N AGFG2 n/a
5 TRCN0000148766 CCTGAAGTAGTATTCCTGCAA pLKO.1 402 CDS 100% 2.640 1.848 N AGFG2 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_017012113.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_06400 pDONR223 100% 61.6% 61.3% None 878_879delTC;883delT;885_886ins546 n/a
2 ccsbBroad304_06400 pLX_304 0% 61.6% 61.3% V5 878_879delTC;883delT;885_886ins546 n/a
3 TRCN0000480883 CCTAAAGTTGCGACTCTAATAAGT pLX_317 30.9% 61.5% 80% V5 (not translated due to prior stop codon) 878_879delTC;883delT;885_886ins547 n/a
4 ccsbBroadEn_13875 pDONR223 100% 50.3% 48.3% None (many diffs) n/a
5 ccsbBroad304_13875 pLX_304 0% 50.3% 48.3% V5 (many diffs) n/a
6 TRCN0000479605 CACTTCATTTGTATTATAGTATAT pLX_317 82.6% 50.3% 48.3% V5 (many diffs) n/a
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