Construct: ORF ccsbBroad304_06413
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008989.1_s304c1, BRDN0000401993
- Derived from:
- ccsbBroadEn_06413
- DNA Barcode:
- None
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- HSP90AA1 (3320)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_06413
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3320 | HSP90AA1 | heat shock protein 90 alpha... | NM_005348.4 | 81.4% | 69.5% | (many diffs) |
| 2 | human | 3320 | HSP90AA1 | heat shock protein 90 alpha... | XM_011536718.2 | 70.3% | 59.7% | (many diffs) |
| 3 | human | 3320 | HSP90AA1 | heat shock protein 90 alpha... | NM_001017963.3 | 70.3% | 59.6% | (many diffs) |
| 4 | mouse | 15519 | Hsp90aa1 | heat shock protein 90, alph... | NM_010480.5 | 73.7% | 68.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1653
- ORF length:
- 1584
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat gcctgaggaa acccagaccc aagaccaacc gatggaggag gaggaggttg 121 agacgttcgc ctttcaggca gaaattgccc agttgatgtc attgatcatc aatactttct 181 actcgaacaa agagatcttt ctgagagagc tcatttcaaa ttcatcagat gcattggaca 241 aaatccggta tgaaagcttg acagatccca gtaaattaga ctctgggaaa gagctgcata 301 ttaaccttat accgaacaaa caagatcgaa ctctcactat tgtggatact ggaattggaa 361 tgaccaaggc tgacttgatc aataaccttg gtactatcgc caagtctggg accaaagcgt 421 tcatggaagc tttgcaggct ggtgcagata tctctatgat tggccagttc ggtgttggtt 481 tttattctgc ttatttggtt gctgagaaag taactgtgat caccaaacat aacgatgatg 541 agcagtacgc ttgggagtcc tcagcagggg gatcattcac agtgaggaca gacacaggtg 601 aacctatggg tcgtggaaca aaagttatcc tacacctgaa agaagaccaa actgagtact 661 tggaggaacg aagaataaag gagattgtga agaaacattc tcagtttatt ggatatccca 721 ttactctttt tgtggagaag gaacgtgata aagaagtaag cgatgatgag gctgaagaaa 781 aggaagacaa agaagaagaa aaagaaaaag aagagaaaga gtcggaagac aaacctgaaa 841 ttgaagatgt tggttctgat gaggaagaag aaaagaagga tggtgacaag aagaagaaga 901 agaagattaa ggaaaagtac atcgatcaag aagagctcaa caaaacaaag cccatctgga 961 ccagaaatcc cgacgatatt actaatgagg agtacggaga attctataag agcttgacca 1021 atgactggga agatcacttg gcagtgaagc atttttcagt tgaaggacag ttggaattca 1081 gagcccttct atttgtccca cgacgtgctc cttttgatct gtttgaaaac agaaagaaaa 1141 agaacaatat caaattgtat gtacgcagag ttttcatcat ggataactgt gaggagctaa 1201 tccctgaata tctgaacttc attagagggg tggtagactc ggaggatctc cctctaaaca 1261 tatcccgtga gatgttgcaa caaagcaaaa ttttgaaagt tatcaggaag aatttggtca 1321 aaaaatgctt agaactcttt actgaactgg cggaagataa agagaactac aagaaattct 1381 atgagcagtt ctctaaaaac ataaagcttg gaatacacga agactctcaa aatcggaaga 1441 agctttcaga gctgttaagg tactacacat ctgcctctgg tgatgagatg gtttctctca 1501 aggactactg caccagaatg aaggagaacc agaaacatat ctattatatc acaggtgaga 1561 ccaaggacca ggtagctaac tcATCCTATG CAGACAGGGT CTCCTATGGG CTCTGTATAT 1621 TCAGCCCATT TTTGAATAGA ATAGACATCA CCTGACGCTC TACATGCAGA GTCTCCAGGA 1681 TCATTCGCCA CAGCATCCTC AGAACATCCA GTGGGAAGCC AGAGAGACAC AAGCCCTAAG 1741 AGTGAACCTA CCCAGCCAGA GATGTCTATC ATCATATCAC GAAAGACATA CAAGAGACAC 1801 TGTATGCACA GACTGCCAGA TCGTCGTCCG AAACATACAG TCCTTTCACA TGTACACACA 1861 AGCACATAGT GACATACAAT ATCCATGCAG GGAGTCCCAA GAGGTCGACG CGTATGAAGG 1921 GGTTGGATGG CACATCCACA GGCTCTCACA CTTATGGATT CAAGATAAAC CCCCTAGGAA 1981 TCACCTTCAT GACCTTAAGG CAAGAAAAGC CTGCAAATAA GGCAGCCAAG TAGGCGGCCC 2041 AGGACGTGAT CCAGTGTGCC GTGGTTGAGG GGTACTTGAC TCCAAACAGT CTGGAGAAGG 2101 GCCACAGTCA CGTGGTCAGC ATCCTCTGGC CGGTCCAGGC TTGTTATAAC ATCATCAGCG 2161 CCGGGCCTCA TTTCAAGCAC TGCCCAACTT TCGTGTACGA AGAGGTGGCT ATCGGTAAGC 2221 GTGTCCCTAA CCCTTTCCTC GGTGAAGATT TAGAGTAGTA ATGAACTAGT CCGTAACTTG 2281 AAAGTATTTC GATTTCTTGG CCTCTATATA TGTTGTGGAA AGGACGATTA GTTCCATCGT 2341 TATGTCGCCA GGCGCGTTAA GTCGACAATC AACCTCTGGA TTACAAAATT TGTGAAAGAT 2401 TGACTGGTAT TCTTAACTAT GTTGCTCCTT TTACGCTATG TGGATACGCT GCTTTAATGC 2461 CTTGTATCAT GCCAGGCCTT GT