Construct: ORF ccsbBroad304_06418

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF010349.1_s304c1, BRDN0000404368
Derived from:
ccsbBroadEn_06418
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
HTR2B (3357)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_06418
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 3357 HTR2B 5-hydroxytryptamine recepto... NM_000867.5 99.9% 99.7% 1430A>G
2 human 3357 HTR2B 5-hydroxytryptamine recepto... XM_006712482.3 92.8% 92.7% (many diffs)
3 human 3357 HTR2B 5-hydroxytryptamine recepto... NM_001320758.2 91.1% 91% 0_1ins126;1304A>G
4 human 3357 HTR2B 5-hydroxytryptamine recepto... XM_005246520.4 75.1% 75% 0_1ins357;1073A>G
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1509
ORF length:
1443
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatggc tctctcttac agagtgtctg aacttcaaag cacaattcct gagcacattt
121tgcagagcac ctttgttcac gttatctctt ctaactggtc tggattacag acagaatcaa
181taccagagga aatgaaacag attgttgagg aacagggaaa taaactgcac tgggcagctc
241ttctgatact catggtgata atacccacaa ttggtggaaa tacccttgtt attctggctg
301tttcactgga gaagaagctg cagtatgcta ctaattactt tctaatgtcc ttggcggtgg
361ctgatttgct ggttggattg tttgtgatgc caattgccct cttgacaata atgtttgagg
421ctatgtggcc cctcccactt gttctatgtc ctgcctggtt atttcttgac gttctctttt
481caaccgcatc catcatgcat ctctgtgcca tttcagtgga tcgttacata gccatcaaaa
541agccaatcca ggccaatcaa tataactcac gggctacagc attcatcaag attacagtgg
601tgtggttaat ttcaataggc attgccattc cagtccctat taaagggata gagactgatg
661tggacaaccc aaacaatatc acttgtgtgc tgacaaagga acgttttggc gatttcatgc
721tctttggctc actggctgcc ttcttcacac ctcttgcaat tatgattgtc acctactttc
781tcactatcca tgctttacag aagaaggctt acttagtcaa aaacaagcca cctcaacgcc
841taacatggtt gactgtgtct acagttttcc aaagggatga aacaccttgc tcgtcaccgg
901aaaaggtggc aatgctggat ggttctcgaa aggacaaggc tctgcccaac tcaggtgatg
961aaacacttat gcgaagaaca tccacaattg ggaaaaagtc agtgcagacc atttccaacg
1021aacagagagc ctcaaaggtc ctagggattg tgtttttcct ctttttgctt atgtggtgtc
1081ccttctttat tacaaatata actttagttt tatgtgattc ctgtaaccaa actactctcc
1141aaatgctcct ggagatattt gtgtggatag gctatgtttc ctcaggagtg aatcctttgg
1201tctacaccct cttcaataag acatttcggg atgcatttgg ccgatatatc acctgcaatt
1261accgggccac aaagtcagta aaaactctca gaaaacgctc cagtaagatc tacttccgga
1321atccaatggc agagaactct aagtttttca agaaacatgg aattcgaaat gggattaacc
1381ctgccatgta ccagagtcca atgaggctcc gaagttcaac cattcagtct tcatcaatca
1441ttctactaga tacgcttctc ctcactgaaa atgaaggtga caaaactgaa gagcgagtta
1501gttatgtata cccaactttc ttgtacaaag tggttggtaa gcctatccct aaccctctcc
1561tcggtctcga ttctacgtag taatgagcta gcgctaaccg gtggcgcgtt aagtcgacaa
1621tcaacctctg gatta

Download FASTA (ORF) (Full)