Construct: ORF ccsbBroad304_06445
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014768.1_s304c1, BRDN0000410610
- Derived from:
- ccsbBroadEn_06445
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- IL12RB1 (3594)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_06445
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3594 | IL12RB1 | interleukin 12 receptor sub... | NM_153701.3 | 99.7% | 99.7% | 387G>C;641A>G;684C>T |
| 2 | human | 3594 | IL12RB1 | interleukin 12 receptor sub... | XM_011527977.2 | 90.2% | 90.2% | (many diffs) |
| 3 | human | 3594 | IL12RB1 | interleukin 12 receptor sub... | XM_011527976.2 | 61.4% | 59% | (many diffs) |
| 4 | human | 3594 | IL12RB1 | interleukin 12 receptor sub... | NM_001290023.2 | 55.9% | 53.7% | (many diffs) |
| 5 | human | 3594 | IL12RB1 | interleukin 12 receptor sub... | NM_005535.3 | 55.7% | 53.6% | (many diffs) |
| 6 | human | 3594 | IL12RB1 | interleukin 12 receptor sub... | XM_011527975.2 | 52.7% | 50.7% | (many diffs) |
| 7 | human | 3594 | IL12RB1 | interleukin 12 receptor sub... | XM_011527974.2 | 52.7% | 50.6% | (many diffs) |
| 8 | human | 3594 | IL12RB1 | interleukin 12 receptor sub... | NM_001290024.1 | 52.6% | 50.5% | (many diffs) |
| 9 | human | 3594 | IL12RB1 | interleukin 12 receptor sub... | XM_011527973.2 | 52.4% | 50.3% | (many diffs) |
| 10 | human | 3594 | IL12RB1 | interleukin 12 receptor sub... | XM_011527972.3 | 51.9% | 49.9% | (many diffs) |
| 11 | human | 3594 | IL12RB1 | interleukin 12 receptor sub... | XM_011527971.3 | 51.8% | 49.7% | (many diffs) |
| 12 | human | 3594 | IL12RB1 | interleukin 12 receptor sub... | XM_006722741.3 | 51.7% | 49.7% | (many diffs) |
| 13 | human | 3594 | IL12RB1 | interleukin 12 receptor sub... | XM_011527970.2 | 50.9% | 48.9% | (many diffs) |
| 14 | human | 3594 | IL12RB1 | interleukin 12 receptor sub... | XM_011527969.2 | 50.3% | 48.3% | (many diffs) |
| 15 | human | 3594 | IL12RB1 | interleukin 12 receptor sub... | XM_011527968.3 | 50% | 48.1% | (many diffs) |
| 16 | human | 3594 | IL12RB1 | interleukin 12 receptor sub... | XM_011527967.2 | 49.8% | 47.9% | (many diffs) |
| 17 | human | 3594 | IL12RB1 | interleukin 12 receptor sub... | XM_011527966.2 | 49.5% | 47.6% | (many diffs) |
| 18 | human | 3594 | IL12RB1 | interleukin 12 receptor sub... | XM_017026762.1 | 24.1% | 22% | (many diffs) |
| 19 | human | 7576 | ZNF28 | zinc finger protein 28 | NM_001369767.1 | 9.8% | 8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1209
- ORF length:
- 1143
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgga gccgctggtg acctgggtgg tccccctcct cttcctcttc ctgctgtcca 121 ggcagggcgc tgcctgcaga accagtgagt gctgttttca ggacccgcca tatccggatg 181 cagactcagg ctcggcctcg ggccctaggg acctgagatg ctatcggata tccagtgatc 241 gttacgagtg ctcctggcag tatgagggtc ccacagctgg ggtcagccac ttcctgcggt 301 gttgccttag ctccgggcgc tgctgctact tcgccgccgg ctcagccacc aggctgcagt 361 tctccgacca ggctggggtg tctgtgctgt acactgtcac actctgggtg gaatcctggg 421 ccaggaacca gacagagaag tctcctgagg tcaccctgca gctctacaac tcagttaaat 481 atgagcctcc tctgggagac atcaaggtgt ccaagttggc cgggcagctg cgtatggagt 541 gggagacccc ggataaccag gttggtgctg aggtgcagtt ccggcaccgg acacccagca 601 gcccatggaa gttgggcgac tgcggacctc aggatgatga tactgagtcc tgcctctgcc 661 ccctggagat gaatgtggcc caggaattcc agctccgacg acggcggctg gggagccaag 721 gaagttcctg gagcaagtgg agcagccctg tgtgcgttcc ccctgaaaac cccccacagc 781 ctcaggtgag attctcggtg gagcagctgg gccaggatgg gaggaggcgg ctgaccctga 841 aagagcagcc aacccagctg gagcttccag aaggctgtca agggctggcg cctggcacgg 901 aggtcactta ccgactacag ctccacatgc tgtcctgccc gtgtaaggcc aaggccacca 961 ggaccctgca cctggggaag atgccctatc tctcgggtgc tgcctacaac gtggctgtca 1021 tctcctcgaa ccaatttggt cctggcctga accagacgtg gcacattcct gccgacaccc 1081 acacagatgg catgatctca gctcactgca acctccgcct tccagattca agagattctc 1141 ctgcttcagc ctcccgagta gctgggatta caggcatctg ccaccatacc cggctaattt 1201 tgtattttta cccaactttc ttgtacaaag tggttggtaa gcctatccct aaccctctcc 1261 tcggtctcga ttctacgtag taatgagcta gcgctaaccg gtggcgcgtt aagtcgacaa 1321 tcaacctctg gatta