Construct: ORF ccsbBroad304_06459
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002494.1_s304c1, BRDN0000402994
- Derived from:
- ccsbBroadEn_06459
- DNA Barcode:
- None
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- IRF4 (3662)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_06459
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3662 | IRF4 | interferon regulatory factor 4 | NM_002460.4 | 99.7% | 54.4% | 421C>T;751_752insG;823_824insG |
| 2 | human | 3662 | IRF4 | interferon regulatory factor 4 | NM_001195286.2 | 99.5% | 54.2% | (many diffs) |
| 3 | human | 3662 | IRF4 | interferon regulatory factor 4 | XM_006715090.2 | 91.8% | 51.9% | (many diffs) |
| 4 | human | 3662 | IRF4 | interferon regulatory factor 4 | NR_046000.3 | 24.7% | (many diffs) | |
| 5 | mouse | 16364 | Irf4 | interferon regulatory factor 4 | NM_013674.2 | 87.3% | 50.9% | (many diffs) |
| 6 | mouse | 16364 | Irf4 | interferon regulatory factor 4 | NM_001347508.1 | 87.1% | 50.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 990
- ORF length:
- 924
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgaa cctggagggc ggcggccgag gcggagagtt cggcatgagc gcggtgagct 121 gcggcaacgg gaagctccgc cagtggctga tcgaccagat cgacagcggc aagtaccccg 181 ggctggtgtg ggagaacgag gagaagagca tcttccgcat cccctggaag cacgcgggca 241 agcaggacta caaccgcgag gaggacgccg cgctcttcaa ggcttgggca ctgtttaaag 301 gaaagttccg agaaggcatc gacaagccgg accctcccac ctggaagacg cgcctgcggt 361 gcgctttgaa caagagcaat gactttgagg aactggttga gcggagccag ctggacatct 421 cagacccgta caaagtgtac aggattgttc ctgagggagc caaaaaagga gccaagcagc 481 tcaccttgga ggacccgcag atgtccatga gccaccccta caccatgaca acgccttacc 541 cttcgctccc agcccagcag gttcacaact acatgatgcc acccctcgac cgaagctgga 601 gggactacgt cccggatcag ccacacccgg aaatcccgta ccaatgtccc atgacgtttg 661 gaccccgcgg ccaccactgg caaggcccag cttgtgaaaa tggttgccag gtgacaggaa 721 ccttttatgc ttgtgcccca cctgagtccc aggctcccgg agtccccaca gagccaagca 781 taaggtctGC CGAAGCCTTG GCGTTCTCAG ACTGCCGGGC TGCACATCTG CCTGTACTAC 841 CGGGAAATCC TCGTGAAGGA GCTGACCACG TCCAGCCCCG AGGGCTGCCG GGATCTCCCA 901 TGGACATACG TATGACGCCA GCAACCTGGA CCAGGTCCTG TTCCCCTACC CAGAGGACAA 961 TGGCCAGAGG AAAAACATTG AGAAGCTGCT GAGCCACCTG GAGAGGGGCG TGGTCCTCTG 1021 GATGGCCCCC GACGGGCTCT ATGCGAAAAG ACTGTGCCAG AGCAGGATCT ACTGGGACGG 1081 GCCCCTGGCG CTGTGCAACG ACCGGCCCAA CAAACTGGAG AGAGACCAGA CCTGCAAGCT 1141 CTTTGACACA CAGCAGTTCT TGTCAGAGCT GCAAGCGTTT GCTCACCACG GCCGCTCCCT 1201 GCCAAGATTC CAGGTGACTC TATGCTTTGG AGAGGAGTTT CCAGACCCTC AGAGGCAAAG 1261 AAAGCTCATC ACAGCTCACG TAGAACCTCT GCTAGCCAGA CAACTATATT ATTTTGCTCA 1321 ACAAAACAGT GGACATTTCC TGAGGGGCTA CGATTTACCA GAACACATCA GCAATCCAGA 1381 AGATTACCAC AGATCTATCC GCCATTCCTC TATTCAAGAA TGCCCAACTT TCTTGTACaa 1441 agtggttggt aagcctatcc ctaaccctct cctcggtctc gattctacgt agtaatgagc 1501 tagcgctaac cggtggcgcg ttaagtcgac aatcaacctc tggatta