Construct: ORF ccsbBroad304_06478
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012674.1_s304c1, BRDN0000461342
- Derived from:
- ccsbBroadEn_06478
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- KCNJ6 (3763)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_06478
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3763 | KCNJ6 | potassium inwardly rectifyi... | NM_002240.5 | 99.9% | 100% | 495A>G |
| 2 | mouse | 16522 | Kcnj6 | potassium inwardly-rectifyi... | NM_010606.2 | 89.8% | 98.8% | (many diffs) |
| 3 | mouse | 16522 | Kcnj6 | potassium inwardly-rectifyi... | NM_001025584.2 | 87.3% | 96.2% | (many diffs) |
| 4 | mouse | 16522 | Kcnj6 | potassium inwardly-rectifyi... | XM_011246104.1 | 86.9% | 95.5% | (many diffs) |
| 5 | mouse | 16522 | Kcnj6 | potassium inwardly-rectifyi... | NM_001025585.2 | 68.4% | 74.5% | (many diffs) |
| 6 | mouse | 16522 | Kcnj6 | potassium inwardly-rectifyi... | NM_001025590.1 | 64.4% | 70.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1338
- ORF length:
- 1269
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat ggccaagctg acagaatcca tgactaacgt cctggagggc gactccatgg 121 atcaggacgt cgaaagccca gtggccattc accagccaaa gttgcctaag caggccaggg 181 atgacctgcc aagacacatc agccgagatc ggaccaaaag gaaaatccag aggtacgtga 241 ggaaagacgg aaagtgcaat gttcatcacg gcaacgtgag ggagacctat cgctacctga 301 ccgatatctt caccacatta gtggacctga agtggagatt caacctattg atttttgtca 361 tggtttacac agtgacctgg ctcttttttg gaatgatctg gtggttgatc gcatacatac 421 ggggagacat ggaccacata gaggacccct cctggactcc ttgtgttacc aacctcaacg 481 ggttcgtctc tgctttttta ttctcaatag agacagaaac caccattggt tatggctacc 541 gggtcatcac agataaatgc ccggagggaa ttattcttct cttaatccaa tctgtgttgg 601 ggtccattgt caatgcattc atggtgggat gcatgtttgt aaaaatctct caacccaaga 661 agagggcaga gaccctggtc ttttccaccc atgcagtgat ctccatgcgg gatgggaaac 721 tgtgcctgat gttccgggta ggggacctta ggaattccca cattgtggag gcttccatca 781 gagccaagtt gatcaaatcc aaacagacct cggaggggga gttcatcccg ttgaaccaga 841 cggatatcaa cgtagggtat tacacggggg atgaccgtct gtttctggtg tcaccgctga 901 tcattagcca tgaaattaac caacagagtc ctttctggga gatctccaaa gcccagctgc 961 ccaaagagga actggaaatt gtggtcatcc tagaaggaat ggtggaagcc acagggatga 1021 catgccaagc tcgaagctcc tacatcacca gtgagatcct gtggggttac cggttcacac 1081 ctgtcctgac cctggaggac gggttctacg aagttgacta caacagcttc catgagacct 1141 atgagaccag caccccatcc cttagtgcca aagagctggc cgagttagcc agcagggcag 1201 agctgcccct gagttggtct gtatccagca aactcaacca acatgcagaa ctggagactg 1261 aagaggaaga aaagaacctc gaagagcaaa cagaaagaaa tggtgatgtg gcaaacctgg 1321 agaatgaatc caaagttttg ccaactttct tgtacaaagt ggttggtaag cctatcccta 1381 accctctcct cggtctcgat tctacgtagt aatgagctag cgctaaccgg tggcgcgtta 1441 agtcgacaat caacctctgg atta