Construct: ORF ccsbBroad304_06551

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF011309.1_s304c1, BRDN0000406289
Derived from:
ccsbBroadEn_06551
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
MAGEA1 (4100)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_06551
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 4100 MAGEA1 MAGE family member A1 NM_004988.5 99.7% 99.6% 94A>G;813C>T
2 human 4103 MAGEA4 MAGE family member A4 NM_001011548.1 85.1% 75% (many diffs)
3 human 4103 MAGEA4 MAGE family member A4 NM_001011549.1 85.1% 75% (many diffs)
4 human 4103 MAGEA4 MAGE family member A4 NM_001011550.1 85.1% 75% (many diffs)
5 human 4103 MAGEA4 MAGE family member A4 NM_002362.4 85.1% 75% (many diffs)
6 human 4103 MAGEA4 MAGE family member A4 XM_005274678.3 85.1% 75% (many diffs)
7 human 4103 MAGEA4 MAGE family member A4 XM_005274679.4 85.1% 75% (many diffs)
8 human 4103 MAGEA4 MAGE family member A4 XM_005274677.2 68% 59.9% (many diffs)
9 human 100533997 MAGEA10-MAGEA5 MAGEA10-MAGEA5 readthrough NM_001204811.3 31.6% 26.2% (many diffs)
10 human 4104 MAGEA5 MAGE family member A5 NM_021049.4 31.6% 26.2% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
993
ORF length:
927
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatgtc tcttgagcag aggagtctgc actgcaagcc tgaggaagcc cttgaggccc
121aacaagaggc cctgggcctg gtgtgtgtgc aggctgccgc ctcctcctcc tctcctctgg
181tcctgggcac cctggaggag gtgcccactg ctgggtcaac agatcctccc cagagtcctc
241agggagcctc cgcctttccc actaccatca acttcactcg acagaggcaa cccagtgagg
301gttccagcag ccgtgaagag gaggggccaa gcacctcttg tatcctggag tccttgttcc
361gagcagtaat cactaagaag gtggctgatt tggttggttt tctgctcctc aaatatcgag
421ccagggagcc agtcacaaag gcagaaatgc tggagagtgt catcaaaaat tacaagcact
481gttttcctga gatcttcggc aaagcctctg agtccttgca gctggtcttt ggcattgacg
541tgaaggaagc agaccccacc ggccactcct atgtccttgt cacctgccta ggtctctcct
601atgatggcct gctgggtgat aatcagatca tgcccaagac aggcttcctg ataattgtcc
661tggtcatgat tgcaatggag ggcggccatg ctcctgagga ggaaatctgg gaggagctga
721gtgtgatgga ggtgtatgat gggagggagc acagtgccta tggggagccc aggaagctgc
781tcacccaaga tttggtgcag gaaaagtacc tggagtaccg gcaggtgccg gacagtgatc
841ccgcacgcta tgagttcctg tggggtccaa gggcccttgc tgaaaccagc tatgtgaaag
901tccttgagta tgtgatcaag gtcagtgcaa gagttcgctt tttcttccca tccctgcgtg
961aagcagcttt gagagaggag gaagagggag tctgcccaac tttcttgtac aaagtggttg
1021gtaagcctat ccctaaccct ctcctcggtc tcgattctac gtagtaatga gctagcgcta
1081accggtggcg cgttaagtcg acaatcaacc tctggatta

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