Construct: ORF ccsbBroad304_06551
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011309.1_s304c1, BRDN0000406289
- Derived from:
- ccsbBroadEn_06551
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MAGEA1 (4100)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_06551
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 4100 | MAGEA1 | MAGE family member A1 | NM_004988.5 | 99.7% | 99.6% | 94A>G;813C>T |
| 2 | human | 4103 | MAGEA4 | MAGE family member A4 | NM_001011548.1 | 85.1% | 75% | (many diffs) |
| 3 | human | 4103 | MAGEA4 | MAGE family member A4 | NM_001011549.1 | 85.1% | 75% | (many diffs) |
| 4 | human | 4103 | MAGEA4 | MAGE family member A4 | NM_001011550.1 | 85.1% | 75% | (many diffs) |
| 5 | human | 4103 | MAGEA4 | MAGE family member A4 | NM_002362.4 | 85.1% | 75% | (many diffs) |
| 6 | human | 4103 | MAGEA4 | MAGE family member A4 | XM_005274678.3 | 85.1% | 75% | (many diffs) |
| 7 | human | 4103 | MAGEA4 | MAGE family member A4 | XM_005274679.4 | 85.1% | 75% | (many diffs) |
| 8 | human | 4103 | MAGEA4 | MAGE family member A4 | XM_005274677.2 | 68% | 59.9% | (many diffs) |
| 9 | human | 100533997 | MAGEA10-MAGEA5 | MAGEA10-MAGEA5 readthrough | NM_001204811.3 | 31.6% | 26.2% | (many diffs) |
| 10 | human | 4104 | MAGEA5 | MAGE family member A5 | NM_021049.4 | 31.6% | 26.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 993
- ORF length:
- 927
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgtc tcttgagcag aggagtctgc actgcaagcc tgaggaagcc cttgaggccc 121 aacaagaggc cctgggcctg gtgtgtgtgc aggctgccgc ctcctcctcc tctcctctgg 181 tcctgggcac cctggaggag gtgcccactg ctgggtcaac agatcctccc cagagtcctc 241 agggagcctc cgcctttccc actaccatca acttcactcg acagaggcaa cccagtgagg 301 gttccagcag ccgtgaagag gaggggccaa gcacctcttg tatcctggag tccttgttcc 361 gagcagtaat cactaagaag gtggctgatt tggttggttt tctgctcctc aaatatcgag 421 ccagggagcc agtcacaaag gcagaaatgc tggagagtgt catcaaaaat tacaagcact 481 gttttcctga gatcttcggc aaagcctctg agtccttgca gctggtcttt ggcattgacg 541 tgaaggaagc agaccccacc ggccactcct atgtccttgt cacctgccta ggtctctcct 601 atgatggcct gctgggtgat aatcagatca tgcccaagac aggcttcctg ataattgtcc 661 tggtcatgat tgcaatggag ggcggccatg ctcctgagga ggaaatctgg gaggagctga 721 gtgtgatgga ggtgtatgat gggagggagc acagtgccta tggggagccc aggaagctgc 781 tcacccaaga tttggtgcag gaaaagtacc tggagtaccg gcaggtgccg gacagtgatc 841 ccgcacgcta tgagttcctg tggggtccaa gggcccttgc tgaaaccagc tatgtgaaag 901 tccttgagta tgtgatcaag gtcagtgcaa gagttcgctt tttcttccca tccctgcgtg 961 aagcagcttt gagagaggag gaagagggag tctgcccaac tttcttgtac aaagtggttg 1021 gtaagcctat ccctaaccct ctcctcggtc tcgattctac gtagtaatga gctagcgcta 1081 accggtggcg cgttaagtcg acaatcaacc tctggatta