Transcript: Human NM_021049.4

Homo sapiens MAGE family member A5 (MAGEA5), mRNA.

Source:
NCBI, updated 2019-09-23
Taxon:
Homo sapiens (human)
Gene:
MAGEA5 (4104)
Length:
1664
CDS:
173..547

Additional Resources:

NCBI RefSeq record:
NM_021049.4
NBCI Gene record:
MAGEA5 (4104)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_021049.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000416970 TGGCCTGGTGGTTGATAATAA pLKO_005 731 3UTR 100% 15.000 7.500 Y MAGEA5 n/a
2 TRCN0000282137 GCCGGTCACAAAGGCAGAAAT pLKO_005 556 3UTR 100% 13.200 6.600 Y MAGEA9B n/a
3 TRCN0000420026 AGAAGGTGGCTGACTTGATTC pLKO_005 504 CDS 100% 10.800 5.400 Y MAGEA5 n/a
4 TRCN0000139693 CAGTAAGAAGGTGGCTGACTT pLKO.1 499 CDS 100% 4.950 2.475 Y MAGEA5 n/a
5 TRCN0000166663 CCCAAGATTTGGTGCAGGAAA pLKO.1 913 3UTR 100% 4.950 2.475 Y MAGEA1 n/a
6 TRCN0000139580 CCCTGCCTTAATGTGACATGA pLKO.1 1189 3UTR 100% 4.950 2.475 Y MAGEA5 n/a
7 TRCN0000141221 CTGCCATCGATTTCACTCTAT pLKO.1 390 CDS 100% 4.950 2.475 Y MAGEA5 n/a
8 TRCN0000128375 GAATGACAGTAGTCACACATA pLKO.1 1366 3UTR 100% 4.950 2.475 Y MAGEA3 n/a
9 TRCN0000144761 GATTGGGAAATCCATTCCATT pLKO.1 1432 3UTR 100% 4.950 2.475 Y MAGEA2B n/a
10 TRCN0000152384 GATTGGGAAATCCATTCCATT pLKO.1 1432 3UTR 100% 4.950 2.475 Y MAGEA6 n/a
11 TRCN0000145472 GCAGTCAACATTCTTAGTAGT pLKO.1 1236 3UTR 100% 4.950 2.475 Y MAGEA5 n/a
12 TRCN0000422039 ACTCGCTGCTTGAAAGTACTG pLKO_005 1004 3UTR 100% 4.050 2.025 Y MAGEA5 n/a
13 TRCN0000436948 TCACCAGGTCCTCTCAAGAGT pLKO_005 344 CDS 100% 3.000 1.500 Y MAGEA5 n/a
14 TRCN0000139290 CAAAGGCAGAAATGCTGGAGA pLKO.1 564 3UTR 100% 2.640 1.320 Y MAGEA4 n/a
15 TRCN0000144376 CAACATTCTTAGTAGTGGGTT pLKO.1 1241 3UTR 100% 2.640 1.320 Y MAGEA5 n/a
16 TRCN0000141849 GATTTCACTCTATGGAGGCAA pLKO.1 398 CDS 100% 2.640 1.320 Y MAGEA5 n/a
17 TRCN0000139120 CCTGATAATCGTCTTGGGCAT pLKO.1 776 3UTR 100% 2.160 1.080 Y MAGEA5 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_021049.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_00967 pDONR223 100% 100% 100% None n/a
2 ccsbBroad304_00967 pLX_304 0% 100% 100% V5 n/a
3 TRCN0000474004 TTCCCTTAAAATCATCCATACATA pLX_317 58.4% 100% 100% V5 n/a
4 ccsbBroadEn_06552 pDONR223 100% 34.5% 30.5% None (many diffs) n/a
5 ccsbBroad304_06552 pLX_304 0% 34.5% 30.5% V5 (many diffs) n/a
6 TRCN0000472116 CAAACACCCAATCCGTACCTTTCC pLX_317 36.8% 34.5% 30.5% V5 (many diffs) n/a
7 ccsbBroadEn_06551 pDONR223 100% 31.6% 26.2% None (many diffs) n/a
8 ccsbBroad304_06551 pLX_304 0% 31.6% 26.2% V5 (many diffs) n/a
9 TRCN0000480348 CATGATCCTTACGCAAACTTAGCG pLX_317 45.8% 31.6% 26.2% V5 (many diffs) n/a
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