Construct: ORF ccsbBroad304_06574

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF018495.1_s304c1, BRDN0000406540
Derived from:
ccsbBroadEn_06574
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
MDH2 (4191)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_06574
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 4191 MDH2 malate dehydrogenase 2 NM_005918.4 99.9% 99.7% 26C>T
2 human 4191 MDH2 malate dehydrogenase 2 NM_001282403.2 87.4% 87.2% 26C>T;428_429ins126
3 human 4191 MDH2 malate dehydrogenase 2 NM_001282404.2 68.3% 68.3% 0_1ins321
4 mouse 17448 Mdh2 malate dehydrogenase 2, NAD... NM_008617.2 85.2% 94.6% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1080
ORF length:
1014
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatgct ctccgccctc gcccggcctg tcagcgctgc tctccgccgc agcttcagca
121cctcggccca gaacaatgct aaagtagctg tgctaggggc ctctggaggc atcgggcagc
181cactttcact tctcctgaag aacagcccct tggtgagccg cctgaccctc tatgatatcg
241cgcacacacc cggagtggcc gcagatctga gccacatcga gaccaaagcc gctgtgaaag
301gctacctcgg acctgaacag ctgcctgact gcctgaaagg ttgtgatgtg gtagttattc
361cggctggagt ccccagaaag ccaggcatga cccgggacga cctgttcaac accaatgcca
421cgattgtggc caccctgacc gctgcctgtg cccagcactg cccggaagcc atgatctgcg
481tcattgccaa tccggttaat tccaccatcc ccatcacagc agaagttttc aagaagcatg
541gagtgtacaa ccccaacaaa atcttcggcg tgacgaccct ggacatcgtc agagccaaca
601cctttgttgc agagctgaag ggtttggatc cagctcgagt caacgtccct gtcattggtg
661gccatgctgg gaagaccatc atccccctga tctctcagtg cacccccaag gtggactttc
721cccaggacca gctgacagca ctcactgggc ggatccagga ggccggcacg gaggtggtca
781aggctaaagc cggagcaggc tctgccaccc tctccatggc gtatgccggc gcccgctttg
841tcttctccct tgtggatgca atgaatggaa aggaaggtgt tgtggaatgt tccttcgtta
901agtcacagga aacggaatgt acctacttct ccacaccgct gctgcttggg aaaaagggca
961tcgagaagaa cctgggcatc ggcaaagtct cctcttttga ggagaagatg atctcggatg
1021ccatccccga gctgaaggcc tccatcaaga agggggaaga tttcgtgaag accctgaagt
1081gcccaacttt cttgtacaaa gtggttggta agcctatccc taaccctctc ctcggtctcg
1141attctacgta gtaatgagct agcgctaacc ggtggcgcgt taagtcgaca atcaacctct
1201ggatta

Download FASTA (ORF) (Full)