Construct: ORF ccsbBroad304_06595

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF015637.1_s304c1, BRDN0000399216
Derived from:
ccsbBroadEn_06595
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
MSH4 (4438)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_06595
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 4438 MSH4 mutS homolog 4 NM_002440.4 99.8% 99.8% (many diffs)
2 mouse 55993 Msh4 mutS homolog 4 NM_031870.3 81% 81.7% (many diffs)
3 mouse 55993 Msh4 mutS homolog 4 NM_001282054.1 69.9% 75.3% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
2874
ORF length:
2808
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatgct gaggcctgag atctcatcaa cctcgccttc tgccccggcg gtttccccgt
121cgtcgggaga aacccgctca cctcagggtc cccgctacaa tttcggactc caggagactc
181cacagagccg cccttcggtc caggtggtct ctgcatccac ctgtcctggc acgtcaggag
241ctgcgggcga ccggagcagc agcagcagca gccttccctg ccccgcgcca aactcccggc
301cagctcaagg ttcatacttt ggaaacaaaa gagcttatgc agagaacaca gttgcatcaa
361attttacttt tggtgcaagc tcatcttctg cacgagatac taattatcct caaacactta
421aaactccatt gtctactgga aatcctcaga gatcaggtta taagagctgg acaccacaag
481tgggatattc agcttcatcc tcatctgcga tttctgcaca ctccccatca gttattgtag
541ctgttgtaga agggagagga cttgccagag gtgaaatagg aatggcaagt attgatttaa
601aaaaccccca aattatacta tcccagtttg cagacaacac aacatatgca aaggtgatca
661ctaaacttaa aattttatca cctttggaaa taataatgtc aaatactgct tgtgctgtgg
721ggaattccac caagttgttc actctgatca cagaaaattt caagaatgtt aatttcacta
781ctatccaaag gaaatacttc aatgaaacaa aaggattaga gtacattgaa cagttatgca
841tagcagaatt cagcactgtc ctaatggagg ttcagtccaa gtattactgc cttgcagctg
901ttgcagcttt gttaaaatat gttgaattta ttcaaaattc agtttatgca ccaaaatcac
961tgaagatttg tttccagggt agtgaacaga cagccatgat agattcatca tcagcccaaa
1021accttgaatt gttaattaat aatcaagact ataggaataa tcacactctc tttggtgttc
1081taaattatac taagactcct ggagggagta gacgacttcg ttctaatata ttagagcctc
1141tagttgatat tgaaaccgtt aacatgagat tagattgtgt tcaagaacta cttcaagatg
1201aggaactatt ttttggactt caatcagtta tatcaagatt tcttgataca gagcagcttc
1261tttctgtttt agtccaaatt ccaaagcaag acacggtcaa tgctgctgaa tcaaagataa
1321caaatttaat atacttaaaa cataccttgg aacttgtgga tcctttaaag attgctatga
1381agaactgtaa cacaccttta ttaagagctt actatggttc cttggaagac aagaggtttg
1441gaatcatact tgaaaagatt aaaacagtaa ttaatgatga tgcaagatac atgaaaggat
1501gcctaaacat gaggactcag aagtgctatg cagtgaggtc taacataaat gaatttcttg
1561acatagcaag aagaacatac acagagattg tagatgacat agcaggaatg atatcacaac
1621ttggagaaaa atacagtctt cctttaagga caagttttag ctctgctcga ggatttttca
1681tccagatgac tacagattgt atagccctac ctagtgatca acttccttca gaatttatta
1741agatttctaa agtgaaaaat tcttacagct ttacatcagc agatttaatt aaaatgaatg
1801aaagatgcca agaatctttg agagaaatct atcacatgac ttatatgata gtgtgcaaac
1861tgcttagtga gatttatgaa catattcatt gcttatataa actatctgac actgtgtcaa
1921tgctggatat gctactgtca tttgctcatg cctgcactct ttctgactat gttcgaccag
1981aatttactga tactttagca atcaaacagg gatggcatcc tattcttgaa aaaatatctg
2041cggaaaaacc tattgccaac aatacctatg ttacagaagg gagtaatttt ttgatcataa
2101ctggaccaaa catgagtgga aaatccacat atttaaaaca gattgctctt tgtcagatta
2161tggcccagat tggatcatat gttccagcag aatattcttc ctttagaatt gctaaacaga
2221tttttacaag aattagtact gatgatgata tcgaaacaaa ttcatcaaca tttatgaaag
2281aaatgaaaga gatagcatat attctacata atgctaatga caaatcgctc atattaattg
2341atgaacttgg cagaggtact aatacggaag aaggtattgg catttgttat gctgtttgtg
2401aatatctact gagcttaaag gcatttacac tgtttgctac acatttcctg gaactatgcc
2461atattgatgc cctgtatcct aatgtagaaa acatgcattt tgaagttcaa catgtaaaga
2521atacctcaag aaataaagaa gcaattttgt atacctacaa actttctaag ggactcacag
2581aagagaaaaa ttatggatta aaagctgcag aggtgtcatc acttccacca tcaattgtct
2641tggatgccaa ggaaatcaca actcaaatta cgagacaaat tttgcaaaac caaaggagta
2701cccctgagat ggaaagacag agagctgtgt accatctagc cactaggctt gttcaaactg
2761ctcgaaactc tcaattggat ccagacagtt tacgaatata tttaagtaac ctcaagaaga
2821agtacaaaga agattttccc aggactgaac aagttccaga aaagactgaa gaatacccaa
2881ctttcttgta caaagtggtt ggtaagccta tccctaaccc tctcctcggt ctcgattcta
2941cgtagtaatg agctagcgct aaccggtggc gcgttaagtc gacaatcaac ctctggatta

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