Construct: ORF ccsbBroad304_06630

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF009685.1_s304c1, BRDN0000461773
Derived from:
ccsbBroadEn_06630
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
NEFM (4741)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_06630
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 4741 NEFM neurofilament medium NM_005382.2 99.9% 99.8% 1315C>A
2 human 4741 NEFM neurofilament medium NM_001105541.2 58.9% 58.8% 0_1ins1128;187C>A
3 mouse 18040 Nefm neurofilament, medium polyp... NM_008691.2 81.2% 83% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
2817
ORF length:
2748
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcaccat gagctacacg ttggactcgc tgggcaaccc gtccgcctac cggcgggtaa
121ccgagacccg ctcgagcttc agccgcgtca gcggctcccc gtccagtggc ttccgctcgc
181agtcgtggtc ccgcggctcg cccagcaccg tgtcctcctc ctataagcgc agcatgctcg
241ccccgcgcct cgcttacagc tcggccatgc tcagctccgc cgagagcagc cttgacttca
301gccagtcctc gtccctgctc aacggcggct ccggacccgg cggcgactac aagctgtccc
361gctccaacga gaaggagcag ctgcaggggc tgaacgaccg ctttgccggc tacatagaga
421aggtgcacta cctggagcag cagaataagg agattgaggc ggagatccag gcgctgcggc
481agaagcaggc ctcgcacgcc cagctgggcg acgcgtacga ccaggagatc cgcgagctgc
541gcgccaccct ggagatggtg aaccacgaga aggctcaggt gcagctggac tcggaccacc
601tggaggaaga catccaccgg ctcaaggagc gctttgagga ggaggcgcgg ttgcgcgacg
661acactgaggc ggccatccgc gcgctgcgca aagacatcga ggaggcgtcg ctggtcaagg
721tggagctgga caagaaggtg cagtcgctgc aggatgaggt ggccttcctg cggagcaacc
781acgaggagga ggtggccgac cttctggccc agatccaggc atcgcacatc acggtggagc
841gcaaagacta cctgaagaca gacatctcga cggcgctgaa ggaaatccgc tcccagctcg
901aaagccactc agaccagaat atgcaccagg ccgaagagtg gttcaaatgc cgctacgcca
961agctcaccga ggcggccgag cagaacaagg aggccatccg ctccgccaag gaagagatcg
1021ccgagtaccg gcgccagctg cagtccaaga gcatcgagct agagtcggtg cgcggcacca
1081aggagtccct ggagcggcag ctcagcgaca tcgaggagcg ccacaaccac gacctcagca
1141gctaccagga caccatccag cagctggaaa atgagcttcg gggcacaaag tgggaaatgg
1201ctcgtcattt gcgcgaatac caggacctcc tcaacgtcaa gatggctctg gatatagaaa
1261tcgctgcgta cagaaaactc ctggagggtg aagagactag atttagcaca tttgcaggaa
1321gcatcactgg gccactgtat acacaccgac ccccaatcac aatatccagt aagattcaga
1381aaaccaaggt ggaagctccc aagcttaagg tccaacacaa atttgtcgag gagatcatag
1441aggaaaccaa agtggaggat gagaagtcag aaatggaaga ggccctgaca gccattacag
1501aggaattggc cgtttccatg aaggaagaga agaaagaagc agcagaagaa aaggaagagg
1561aacccgaagc tgaagaagaa gaagtagctg ccaaaaagtc tccagtgaaa gcaactgcac
1621ctgaagttaa agaagaggaa ggggaaaagg aggaagaaga aggccaggaa gaagaggagg
1681aagaagatga gggagctaag tcagaccaag ccgaagaggg aggatccgag aaggaaggct
1741ctagtgaaaa agaggaaggt gagcaggaag aaggagaaac agaagctgaa gctgaaggag
1801aggaagccga agctaaagag gaaaagaaag tggaggaaaa gagtgaggaa gtggctacca
1861aggaggagct ggtggcagat gccaaggtgg aaaagccaga aaaagccaag tctcctgtgc
1921caaaatcacc agtggaagag aaaggcaagt ctcctgtgcc caagtcacca gtggaagaga
1981aaggcaagtc tcctgtgccc aagtcaccag tggaagagaa aggcaagtct cctgtgccga
2041aatcaccagt ggaagagaaa ggcaagtctc ctgtgtcaaa atcaccagtg gaagagaaag
2101ccaaatctcc tgtgccaaaa tcaccagtgg aagaggcaaa gtcaaaagca gaagtgggga
2161aaggtgaaca gaaagaggaa gaagaaaagg aagtcaagga agctcccaag gaagagaagg
2221tagagaaaaa ggaagagaaa ccaaaggatg tgccagagaa gaagaaagct gagtcccctg
2281taaaggagga agctgtggca gaggtggtca ccatcaccaa atcggtaaag gtgcacttgg
2341agaaagagac caaagaagag gggaagccac tgcagcagga gaaagagaag gagaaagcgg
2401gaggagaggg aggaagtgag gaggaaggga gtgataaagg tgccaaggga tccaggaagg
2461aagacatagc tgtcaatggg gaggtagaag gaaaagagga ggtagagcag gagaccaagg
2521aaaaaggcag tgggagggaa gaggagaaag gcgttgtcac caatggccta gacttgagcc
2581cagcagatga aaagaagggg ggtgataaaa gtgaggagaa agtggtggtg accaaaacgg
2641tagaaaaaat caccagtgag gggggagatg gtgctaccaa atacatcact aaatctgtaa
2701ccgtcactca aaaggttgaa gagcatgaag agacctttga ggagaaacta gtgtctacta
2761aaaaggtaga aaaagtcact tcacacgcca tagtaaagga agtcacccag agtgacttgc
2821caactttctt gtacaaagtg gttggtaagc ctatccctaa ccctctcctc ggtctcgatt
2881ctacgtagta atgagctagc gctaaccggt ggcgcgttaa gtcgacaatc aacctctgga
2941tta

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