Construct: ORF ccsbBroad304_06634

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF009048.1_s304c1, BRDN0000401504
Derived from:
ccsbBroadEn_06634
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
NFATC1 (4772)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_06634
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 4772 NFATC1 nuclear factor of activated... NM_172390.3 99.9% 100% 786T>G;1986A>G
2 human 4772 NFATC1 nuclear factor of activated... NM_001278670.2 97.2% 97.4% (many diffs)
3 human 4772 NFATC1 nuclear factor of activated... NM_001278675.2 95.9% 94.1% (many diffs)
4 human 4772 NFATC1 nuclear factor of activated... NM_001278672.2 93.3% 91.7% (many diffs)
5 human 4772 NFATC1 nuclear factor of activated... XM_017025783.2 89.1% 88.3% 786T>G;1902_1903ins56;1917_1918ins175
6 human 4772 NFATC1 nuclear factor of activated... NM_006162.5 85.8% 84.7% (many diffs)
7 human 4772 NFATC1 nuclear factor of activated... NM_172389.3 82.4% 79.8% (many diffs)
8 human 4772 NFATC1 nuclear factor of activated... NM_001278669.2 75% 74.1% (many diffs)
9 human 4772 NFATC1 nuclear factor of activated... NM_172387.3 72.2% 69.9% (many diffs)
10 human 4772 NFATC1 nuclear factor of activated... NM_172388.3 28.7% 27.5% (many diffs)
11 human 4772 NFATC1 nuclear factor of activated... NM_001278673.1 25.1% 24% (many diffs)
12 mouse 18018 Nfatc1 nuclear factor of activated... NM_001164109.1 82.8% 88% (many diffs)
13 mouse 18018 Nfatc1 nuclear factor of activated... NM_016791.4 79.7% 83.2% (many diffs)
14 mouse 18018 Nfatc1 nuclear factor of activated... NM_198429.2 71.3% 74.9% (many diffs)
15 mouse 18018 Nfatc1 nuclear factor of activated... NM_001164111.1 68.7% 70.9% (many diffs)
16 mouse 18018 Nfatc1 nuclear factor of activated... NM_001164110.1 62.9% 66% (many diffs)
17 mouse 18018 Nfatc1 nuclear factor of activated... NM_001164112.1 60.6% 62.6% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
2217
ORF length:
2148
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcaccat gccaagcacc agctttccag tcccttccaa gtttccactt ggccctgcgg
121ctgcggtctt cgggagagga gaaactttgg ggcccgcgcc gcgcgccggc ggcaccatga
181agtcagcgga ggaagaacac tatggctatg catcctccaa cgtcagcccc gccctgccgc
241tccccacggc gcactccacc ctgccggccc cgtgccacaa ccttcagacc tccacaccgg
301gcatcatccc gccggcggat cacccctcgg ggtacggagc agctttggac ggtgggcccg
361cgggctactt cctctcctcc ggccacacca ggcctgatgg ggcccctgcc ctggagagtc
421ctcgcatcga gataacctcg tgcttgggcc tgtaccacaa caataaccag tttttccacg
481atgtggaggt ggaagacgtc ctccctagct ccaaacggtc cccctccacg gccacgctga
541gtctgcccag cctggaggcc tacagagacc cctcgtgcct gagcccggcc agcagcctgt
601cctcccggag ctgcaactca gaggcctcct cctacgagtc caactactcg tacccgtacg
661cgtcccccca gacgtcgcca tggcagtctc cctgcgtgtc tcccaagacc acggaccccg
721aggagggctt tccccgcggg ctgggggcct gcacactgct gggttccccg cggcactccc
781cctccacctc gccccgcgcc agcgtcactg aggagagctg gctgggtgcc cgctcctcca
841gacccgcgtc cccgtgcaac aagaggaagt acagcctcaa cggccggcag ccgccctact
901caccccacca ctcgcccacg ccgtccccgc acggctcccc gcgggtcagc gtgaccgacg
961actcgtggtt gggcaacacc acccagtaca ccagctcggc catcgtggcc gccatcaacg
1021cgctgaccac cgacagcagc ctggacctgg gagatggcgt ccctgtcaag tcccgcaaga
1081ccaccctgga gcagccgccc tcagtggcgc tcaaggtgga gcccgtcggg gaggacctgg
1141gcagcccccc gcccccggcc gacttcgcgc ccgaagacta ctcctctttc cagcacatca
1201ggaagggcgg cttctgcgac cagtacctgg cggtgccgca gcacccctac cagtgggcga
1261agcccaagcc cctgtcccct acgtcctaca tgagcccgac cctgcccgcc ctggactggc
1321agctgccgtc ccactcaggc ccgtatgagc ttcggattga ggtgcagccc aagtcccacc
1381accgagccca ctacgagacg gagggcagcc ggggggccgt gaaggcgtcg gccggaggac
1441accccatcgt gcagctgcat ggctacttgg agaatgagcc gctgatgctg cagcttttca
1501ttgggacggc ggacgaccgc ctgctgcgcc cgcacgcctt ctaccaggtg caccgcatca
1561cagggaagac cgtgtccacc accagccacg aggccatcct ctccaacacc aaagtcctgg
1621agatcccact cctgccggag aacagcatgc gagccgtcat tgactgtgcc ggaatcctga
1681aactcagaaa ctccgacatt gaacttcgga aaggagagac ggacatcggg aggaagaaca
1741cacgggtacg gctggtgttc cgcgttcacg tcccgcaacc cagcggccgc acgctgtccc
1801tgcaggtggc ctccaacccc atcgaatgct cccagcgctc agctcaggag ctgcctctgg
1861tggagaagca gagcacggac agctatccgg tcgtgggcgg gaagaagatg gtcctgtctg
1921gccacaactt cctgcaggac tccaaggtca ttttcgtgga gaaagcccca gatggccacc
1981atgtctggga gatggaagcg aaaactgacc gggacctgtg caagccgaat tctctggtgg
2041ttgagatccc gccgtttcgg aatcagagga taaccagccc cgttcacgtc agtttctacg
2101tctgcaacgg gaagagaaag cgaagccagt accagcgttt cacctacctt cccgccaacg
2161gtaacgccat ctttctaacc gtaagccgtg aacatgagcg cgtggggtgc tttttcttgc
2221caactttctt gtacaaagtg gttggtaagc ctatccctaa ccctctcctc ggtctcgatt
2281ctacgtagta atgagctagc gctaaccggt ggcgcgttaa gtcgacaatc aacctctgga
2341tta

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