Construct: ORF ccsbBroad304_06653
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008671.1_s304c1, BRDN0000462160
- Derived from:
- ccsbBroadEn_06653
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NPAS2 (4862)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_06653
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 4862 | NPAS2 | neuronal PAS domain protein 2 | NM_002518.4 | 99.8% | 99.8% | 528C>T;1180A>G;2130C>T |
| 2 | human | 4862 | NPAS2 | neuronal PAS domain protein 2 | XM_011511242.2 | 96.2% | 96.2% | (many diffs) |
| 3 | human | 4862 | NPAS2 | neuronal PAS domain protein 2 | XM_005263953.2 | 92.5% | 92.5% | (many diffs) |
| 4 | human | 4862 | NPAS2 | neuronal PAS domain protein 2 | XM_017004214.1 | 87.4% | 87.4% | (many diffs) |
| 5 | human | 4862 | NPAS2 | neuronal PAS domain protein 2 | XM_005263959.2 | 86.3% | 86% | (many diffs) |
| 6 | human | 4862 | NPAS2 | neuronal PAS domain protein 2 | XM_017004215.1 | 86.2% | 85.8% | (many diffs) |
| 7 | human | 4862 | NPAS2 | neuronal PAS domain protein 2 | XM_005263961.4 | 84.8% | 84.8% | (many diffs) |
| 8 | human | 4862 | NPAS2 | neuronal PAS domain protein 2 | XM_017004216.1 | 81.1% | 80.8% | (many diffs) |
| 9 | human | 4862 | NPAS2 | neuronal PAS domain protein 2 | XM_005263960.2 | 80.9% | 80.9% | (many diffs) |
| 10 | human | 4862 | NPAS2 | neuronal PAS domain protein 2 | XM_017004217.1 | 74.7% | 74.4% | (many diffs) |
| 11 | human | 4862 | NPAS2 | neuronal PAS domain protein 2 | XM_011511243.2 | 63% | 61.5% | (many diffs) |
| 12 | mouse | 18143 | Npas2 | neuronal PAS domain protein 2 | NM_008719.2 | 85.1% | 86.9% | (many diffs) |
| 13 | mouse | 18143 | Npas2 | neuronal PAS domain protein 2 | XM_011238452.2 | 85.1% | 86.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2541
- ORF length:
- 2472
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat ggatgaagat gagaaagaca gagccaagag agcttctcga aacaagtctg 121 agaagaagcg tcgggaccag ttcaatgttc tcatcaaaga gctcagttcc atgctccctg 181 gcaacacgcg gaaaatggac aaaaccaccg tgttggaaaa ggtcatcgga tttttgcaga 241 aacacaatga agtctcagcg caaacggaaa tctgtgacat tcagcaagac tggaagcctt 301 cattcctcag taatgaagaa ttcacccagc tgatgttgga ggcattagat ggcttcatta 361 tcgcagtgac aacagacggc agcatcatct atgtctctga cagtatcacg cctctccttg 421 ggcatttacc gtcggatgtc atggatcaga atttgttaaa tttcctccca gaacaagaac 481 attcagaagt ttataaaatc ctttcttccc atatgcttgt gacggattcc ccctccccag 541 aatacttaaa atctgacagc gatttagagt tttattgcca tcttctcaga ggcagtttga 601 acccaaagga atttccaact tatgaataca taaaatttgt aggaaatttt cgctcttaca 661 acaatgtgcc tagcccctcc tgtaatggtt ttgacaacac cctttcaaga ccttgccggg 721 tgccactagg aaaggaggtt tgcttcattg ccaccgttcg tctggcaaca ccacaattct 781 taaaggaaat gtgcatagtt gacgaacctt tagaggaatt cacttcaagg catagcttgg 841 aatggaaatt tttatttctg gatcacagag cacctccaat cataggatac ctgccttttg 901 aagtgctggg aacctcaggc tatgactact accacattga tgacctggag ctcctggcca 961 ggtgtcacca gcacctgatg cagtttggca aagggaagtc gtgttgctac cggtttctga 1021 ccaaaggtca gcagtggatc tggctgcaga ctcactacta catcacctac catcagtgga 1081 actccaagcc cgagttcatc gtgtgcacac actcggtggt cagttacgca gatgtccggg 1141 tggaaaggag gcaggagctg gctctggaag acccgccatc cgaggccctc cactcctcag 1201 cactaaagga caagggctca agcctggaac ctcggcagca ctttaacgca ctcgacgtgg 1261 gtgcctcggg ccttaatacc agtcattcgc catcggcgtc ctcaagaagt tcccacaaat 1321 cctcgcacac agccatgtca gaacccacct ccactcccac caagctgatg gcagaggcca 1381 gcaccccggc tttgccaaga tcagccaccc tgccccaaga gttacctgtc cccgggctca 1441 gccaggcagc caccatgccg gcccctctgc cttccccatc gtcctgcgac ctcacacagc 1501 agctcctgcc tcagaccgtt ctgcagagca cgcccgctcc catggcacag ttttcggcac 1561 agttcagcat gttccagacc atcaaagacc agctagagca gcggacgcgg atcctgcagg 1621 ccaatatccg gtggcaacag gaagagctcc acaagatcca ggagcagctc tgcctggtcc 1681 aggactccaa cgtccagatg ttcctgcagc agccagctgt atccctgagc ttcagcagca 1741 cccagcgacc tgaggctcag cagcagctac agcaaaggtc agctgcagtg actcagcccc 1801 agctcggggc gggcccccaa cttccagggc agatctcctc tgcccaggtc acaagccagc 1861 acctgctcag agaatcaagt gtgatatcaa cccagggtcc aaagccaatg agaagctcac 1921 agctaatgca gagcagcggc cgctctggaa gcagcctagt gtccccgttc agcagcgcca 1981 cagctgcgct cccgccaagt ctgaatctga ccacacctgc ttccacctcc caggatgcca 2041 gccagtgcca gcccagccca gacttcagcc atgatcggca gctcaggctg ttgctgagcc 2101 agcccatcca gcccatgatg cccgggtcct gtgacgcaag gcagccctcg gaagtcagca 2161 ggacgggacg gcaagtcaag tacgcccaga gccagactgt gtttcaaaat ccagacgcac 2221 accccgccaa cagcagcagc gccccgatgc ccgtcctgct gatggggcag gcggtgctcc 2281 accccagctt ccctgcctcc caaccatcgc ccctgcagcc tgcacaggcc cggcagcagc 2341 caccgcagca ctacctgcag gtacaggcac caacctcttt gcacagtgag cagcaggact 2401 cgctacttct ctccacctac tcacaacagc cagggaccct gggctacccc caaccacccc 2461 cagcacagcc ccagccccta cgtcctcccc gaagggtcag cagtctgtct gagtcgtcag 2521 gcctccagca gccgccccga ttgccaactt tcttgtacaa agtggttggt aagcctatcc 2581 ctaaccctct cctcggtctc gattctacgt agtaatgagc tagcgctaac cggtggcgcg 2641 ttaagtcgac aatcaacctc tggatta