Construct: ORF ccsbBroad304_06708
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014739.1_s304c1, BRDN0000403742
- Derived from:
- ccsbBroadEn_06708
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PDGFRB (5159)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_06708
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5159 | PDGFRB | platelet derived growth fac... | NM_002609.4 | 99.9% | 99.9% | 539C>T |
| 2 | human | 5159 | PDGFRB | platelet derived growth fac... | NM_001355016.2 | 94.1% | 94.1% | 0_1ins192;347C>T |
| 3 | human | 5159 | PDGFRB | platelet derived growth fac... | NM_001355017.2 | 83.5% | 81.8% | 0_1ins517;22C>T;114_147del |
| 4 | mouse | 18596 | Pdgfrb | platelet derived growth fac... | NM_008809.2 | 83.1% | 85.4% | (many diffs) |
| 5 | mouse | 18596 | Pdgfrb | platelet derived growth fac... | NM_001146268.1 | 83% | 85.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 3384
- ORF length:
- 3318
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgcg gcttccgggt gcgatgccag ctctggccct caaaggcgag ctgctgttgc 121 tgtctctcct gttacttctg gaaccacaga tctctcaggg cctggtcgtc acacccccgg 181 ggccagagct tgtcctcaat gtctccagca ccttcgttct gacctgctcg ggttcagctc 241 cggtggtgtg ggaacggatg tcccaggagc ccccacagga aatggccaag gcccaggatg 301 gcaccttctc cagcgtgctc acactgacca acctcactgg gctagacacg ggagaatact 361 tttgcaccca caatgactcc cgtggactgg agaccgatga gcggaaacgg ctctacatct 421 ttgtgccaga tcccaccgtg ggcttcctcc ctaatgatgc cgaggaacta ttcatctttc 481 tcacggaaat aactgagatc accattccat gccgagtaac agacccacag ctggtggtga 541 cactgcacga gaagaaaggg gacgttgcac tgcctgtccc ctatgatcac caacgtggct 601 tttttggtat ctttgaggac agaagctaca tctgcaaaac caccattggg gacagggagg 661 tggattctga tgcctactat gtctacagac tccaggtgtc atccatcaac gtctctgtga 721 acgcagtgca gactgtggtc cgccagggtg agaacatcac cctcatgtgc attgtgatcg 781 ggaatgaggt ggtcaacttc gagtggacat acccccgcaa agaaagtggg cggctggtgg 841 agccggtgac tgacttcctc ttggatatgc cttaccacat ccgctccatc ctgcacatcc 901 ccagtgccga gttagaagac tcggggacct acacctgcaa tgtgacggag agtgtgaatg 961 accatcagga tgaaaaggcc atcaacatca ccgtggttga gagcggctac gtgcggctcc 1021 tgggagaggt gggcacacta caatttgctg agctgcatcg gagccggaca ctgcaggtag 1081 tgttcgaggc ctacccaccg cccactgtcc tgtggttcaa agacaaccgc accctgggcg 1141 actccagcgc tggcgaaatc gccctgtcca cgcgcaacgt gtcggagacc cggtatgtgt 1201 cagagctgac actggttcgc gtgaaggtgg cagaggctgg ccactacacc atgcgggcct 1261 tccatgagga tgctgaggtc cagctctcct tccagctaca gatcaatgtc cctgtccgag 1321 tgctggagct aagtgagagc caccctgaca gtggggaaca gacagtccgc tgtcgtggcc 1381 ggggcatgcc ccagccgaac atcatctggt ctgcctgcag agacctcaaa aggtgtccac 1441 gtgagctgcc gcccacgctg ctggggaaca gttccgaaga ggagagccag ctggagacta 1501 acgtgacgta ctgggaggag gagcaggagt ttgaggtggt gagcacactg cgtctgcagc 1561 acgtggatcg gccactgtcg gtgcgctgca cgctgcgcaa cgctgtgggc caggacacgc 1621 aggaggtcat cgtggtgcca cactccttgc cctttaaggt ggtggtgatc tcagccatcc 1681 tggccctggt ggtgctcacc atcatctccc ttatcatcct catcatgctt tggcagaaga 1741 agccacgtta cgagatccga tggaaggtga ttgagtctgt gagctctgac ggccatgagt 1801 acatctacgt ggaccccatg cagctgccct atgactccac gtgggagctg ccgcgggacc 1861 agcttgtgct gggacgcacc ctcggctctg gggcctttgg gcaggtggtg gaggccacgg 1921 ctcatggcct gagccattct caggccacga tgaaagtggc cgtcaagatg cttaaatcca 1981 cagcccgcag cagtgagaag caagccctta tgtcggagct gaagatcatg agtcaccttg 2041 ggccccacct gaacgtggtc aacctgttgg gggcctgcac caaaggagga cccatctata 2101 tcatcactga gtactgccgc tacggagacc tggtggacta cctgcaccgc aacaaacaca 2161 ccttcctgca gcaccactcc gacaagcgcc gcccgcccag cgcggagctc tacagcaatg 2221 ctctgcccgt tgggctcccc ctgcccagcc atgtgtcctt gaccggggag agcgacggtg 2281 gctacatgga catgagcaag gacgagtcgg tggactatgt gcccatgctg gacatgaaag 2341 gagacgtcaa atatgcagac atcgagtcct ccaactacat ggccccttac gataactacg 2401 ttccctctgc ccctgagagg acctgccgag caactttgat caacgagtct ccagtgctaa 2461 gctacatgga cctcgtgggc ttcagctacc aggtggccaa tggcatggag tttctggcct 2521 ccaagaactg cgtccacaga gacctggcgg ctaggaacgt gctcatctgt gaaggcaagc 2581 tggtcaagat ctgtgacttt ggcctggctc gagacatcat gcgggactcg aattacatct 2641 ccaaaggcag cacctttttg cctttaaagt ggatggctcc ggagagcatc ttcaacagcc 2701 tctacaccac cctgagcgac gtgtggtcct tcgggatcct gctctgggag atcttcacct 2761 tgggtggcac cccttaccca gagctgccca tgaacgagca gttctacaat gccatcaaac 2821 ggggttaccg catggcccag cctgcccatg cctccgacga gatctatgag atcatgcaga 2881 agtgctggga agagaagttt gagattcggc cccccttctc ccagctggtg ctgcttctcg 2941 agagactgtt gggcgaaggt tacaaaaaga agtaccagca ggtggatgag gagtttctga 3001 ggagtgacca cccagccatc cttcggtccc aggcccgctt gcctgggttc catggcctcc 3061 gatctcccct ggacaccagc tccgtcctct atactgccgt gcagcccaat gagggtgaca 3121 acgactatat catccccctg cctgacccca aacccgaggt tgctgacgag ggcccactgg 3181 agggttcccc cagcctagcc agctccaccc tgaatgaagt caacacctcc tcaaccatct 3241 cctgtgacag ccccctggag ccccaggacg aaccagagcc agagccccag cttgagctcc 3301 aggtggagcc ggagccagag ctggaacagt tgccggattc ggggtgccct gcgcctcggg 3361 cggaagcaga ggatagcttc ctgtacccaa ctttcttgta caaagtggtt ggtaagccta 3421 tccctaaccc tctcctcggt ctcgattcta cgtagtaatg agctagcgct aaccggtggc 3481 gcgttaagtc gacaatcaac ctctggatta