Construct: ORF ccsbBroad304_06708

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF014739.1_s304c1, BRDN0000403742
Derived from:
ccsbBroadEn_06708
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
PDGFRB (5159)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_06708
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 5159 PDGFRB platelet derived growth fac... NM_002609.4 99.9% 99.9% 539C>T
2 human 5159 PDGFRB platelet derived growth fac... NM_001355016.2 94.1% 94.1% 0_1ins192;347C>T
3 human 5159 PDGFRB platelet derived growth fac... NM_001355017.2 83.5% 81.8% 0_1ins517;22C>T;114_147del
4 mouse 18596 Pdgfrb platelet derived growth fac... NM_008809.2 83.1% 85.4% (many diffs)
5 mouse 18596 Pdgfrb platelet derived growth fac... NM_001146268.1 83% 85.3% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
3384
ORF length:
3318
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatgcg gcttccgggt gcgatgccag ctctggccct caaaggcgag ctgctgttgc
121tgtctctcct gttacttctg gaaccacaga tctctcaggg cctggtcgtc acacccccgg
181ggccagagct tgtcctcaat gtctccagca ccttcgttct gacctgctcg ggttcagctc
241cggtggtgtg ggaacggatg tcccaggagc ccccacagga aatggccaag gcccaggatg
301gcaccttctc cagcgtgctc acactgacca acctcactgg gctagacacg ggagaatact
361tttgcaccca caatgactcc cgtggactgg agaccgatga gcggaaacgg ctctacatct
421ttgtgccaga tcccaccgtg ggcttcctcc ctaatgatgc cgaggaacta ttcatctttc
481tcacggaaat aactgagatc accattccat gccgagtaac agacccacag ctggtggtga
541cactgcacga gaagaaaggg gacgttgcac tgcctgtccc ctatgatcac caacgtggct
601tttttggtat ctttgaggac agaagctaca tctgcaaaac caccattggg gacagggagg
661tggattctga tgcctactat gtctacagac tccaggtgtc atccatcaac gtctctgtga
721acgcagtgca gactgtggtc cgccagggtg agaacatcac cctcatgtgc attgtgatcg
781ggaatgaggt ggtcaacttc gagtggacat acccccgcaa agaaagtggg cggctggtgg
841agccggtgac tgacttcctc ttggatatgc cttaccacat ccgctccatc ctgcacatcc
901ccagtgccga gttagaagac tcggggacct acacctgcaa tgtgacggag agtgtgaatg
961accatcagga tgaaaaggcc atcaacatca ccgtggttga gagcggctac gtgcggctcc
1021tgggagaggt gggcacacta caatttgctg agctgcatcg gagccggaca ctgcaggtag
1081tgttcgaggc ctacccaccg cccactgtcc tgtggttcaa agacaaccgc accctgggcg
1141actccagcgc tggcgaaatc gccctgtcca cgcgcaacgt gtcggagacc cggtatgtgt
1201cagagctgac actggttcgc gtgaaggtgg cagaggctgg ccactacacc atgcgggcct
1261tccatgagga tgctgaggtc cagctctcct tccagctaca gatcaatgtc cctgtccgag
1321tgctggagct aagtgagagc caccctgaca gtggggaaca gacagtccgc tgtcgtggcc
1381ggggcatgcc ccagccgaac atcatctggt ctgcctgcag agacctcaaa aggtgtccac
1441gtgagctgcc gcccacgctg ctggggaaca gttccgaaga ggagagccag ctggagacta
1501acgtgacgta ctgggaggag gagcaggagt ttgaggtggt gagcacactg cgtctgcagc
1561acgtggatcg gccactgtcg gtgcgctgca cgctgcgcaa cgctgtgggc caggacacgc
1621aggaggtcat cgtggtgcca cactccttgc cctttaaggt ggtggtgatc tcagccatcc
1681tggccctggt ggtgctcacc atcatctccc ttatcatcct catcatgctt tggcagaaga
1741agccacgtta cgagatccga tggaaggtga ttgagtctgt gagctctgac ggccatgagt
1801acatctacgt ggaccccatg cagctgccct atgactccac gtgggagctg ccgcgggacc
1861agcttgtgct gggacgcacc ctcggctctg gggcctttgg gcaggtggtg gaggccacgg
1921ctcatggcct gagccattct caggccacga tgaaagtggc cgtcaagatg cttaaatcca
1981cagcccgcag cagtgagaag caagccctta tgtcggagct gaagatcatg agtcaccttg
2041ggccccacct gaacgtggtc aacctgttgg gggcctgcac caaaggagga cccatctata
2101tcatcactga gtactgccgc tacggagacc tggtggacta cctgcaccgc aacaaacaca
2161ccttcctgca gcaccactcc gacaagcgcc gcccgcccag cgcggagctc tacagcaatg
2221ctctgcccgt tgggctcccc ctgcccagcc atgtgtcctt gaccggggag agcgacggtg
2281gctacatgga catgagcaag gacgagtcgg tggactatgt gcccatgctg gacatgaaag
2341gagacgtcaa atatgcagac atcgagtcct ccaactacat ggccccttac gataactacg
2401ttccctctgc ccctgagagg acctgccgag caactttgat caacgagtct ccagtgctaa
2461gctacatgga cctcgtgggc ttcagctacc aggtggccaa tggcatggag tttctggcct
2521ccaagaactg cgtccacaga gacctggcgg ctaggaacgt gctcatctgt gaaggcaagc
2581tggtcaagat ctgtgacttt ggcctggctc gagacatcat gcgggactcg aattacatct
2641ccaaaggcag cacctttttg cctttaaagt ggatggctcc ggagagcatc ttcaacagcc
2701tctacaccac cctgagcgac gtgtggtcct tcgggatcct gctctgggag atcttcacct
2761tgggtggcac cccttaccca gagctgccca tgaacgagca gttctacaat gccatcaaac
2821ggggttaccg catggcccag cctgcccatg cctccgacga gatctatgag atcatgcaga
2881agtgctggga agagaagttt gagattcggc cccccttctc ccagctggtg ctgcttctcg
2941agagactgtt gggcgaaggt tacaaaaaga agtaccagca ggtggatgag gagtttctga
3001ggagtgacca cccagccatc cttcggtccc aggcccgctt gcctgggttc catggcctcc
3061gatctcccct ggacaccagc tccgtcctct atactgccgt gcagcccaat gagggtgaca
3121acgactatat catccccctg cctgacccca aacccgaggt tgctgacgag ggcccactgg
3181agggttcccc cagcctagcc agctccaccc tgaatgaagt caacacctcc tcaaccatct
3241cctgtgacag ccccctggag ccccaggacg aaccagagcc agagccccag cttgagctcc
3301aggtggagcc ggagccagag ctggaacagt tgccggattc ggggtgccct gcgcctcggg
3361cggaagcaga ggatagcttc ctgtacccaa ctttcttgta caaagtggtt ggtaagccta
3421tccctaaccc tctcctcggt ctcgattcta cgtagtaatg agctagcgct aaccggtggc
3481gcgttaagtc gacaatcaac ctctggatta

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