Transcript: Mouse NM_001146268.1

Mus musculus platelet derived growth factor receptor, beta polypeptide (Pdgfrb), transcript variant 1, mRNA.

Source:
NCBI, updated 2017-05-21
Taxon:
Mus musculus (mouse)
Gene:
Pdgfrb (18596)
Length:
5413
CDS:
430..3729

Additional Resources:

NCBI RefSeq record:
NM_001146268.1
NBCI Gene record:
Pdgfrb (18596)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_001146268.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000350692 GTACGGTGGTGTGTTCATATC pLKO_005 4050 3UTR 100% 10.800 15.120 N Pdgfrb n/a
2 TRCN0000078768 CCATGCTTAATGAATGCTGTT pLKO.1 4250 3UTR 100% 4.050 5.670 N Pdgfrb n/a
3 TRCN0000078770 CCGGAACAAACACACCTTCTT pLKO.1 2511 CDS 100% 4.950 3.960 N Pdgfrb n/a
4 TRCN0000078771 CGACCAGTTCTACAATGCCAT pLKO.1 3159 CDS 100% 2.640 2.112 N Pdgfrb n/a
5 TRCN0000321929 GATGTCACTGAGACGACAATT pLKO_005 847 CDS 100% 13.200 9.240 N Pdgfrb n/a
6 TRCN0000321931 GTGAGAGGAAGCGTATCTATA pLKO_005 758 CDS 100% 13.200 9.240 N Pdgfrb n/a
7 TRCN0000321930 GAAGCGGGCTACTATACTATG pLKO_005 1594 CDS 100% 10.800 7.560 N Pdgfrb n/a
8 TRCN0000078772 CCAGTTCTACAATGCCATCAA pLKO.1 3162 CDS 100% 4.950 3.465 N Pdgfrb n/a
9 TRCN0000078769 CCTTGAATGAAGTCAACACTT pLKO.1 3572 CDS 100% 4.950 3.465 N Pdgfrb n/a
10 TRCN0000321932 CTTCTGGGTGAAGGCTATAAA pLKO_005 3310 CDS 100% 15.000 9.000 N Pdgfrb n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001146268.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_14736 pDONR223 0% 83% 85.3% None (many diffs) n/a
2 ccsbBroad304_14736 pLX_304 0% 83% 85.3% V5 (many diffs) n/a
3 TRCN0000469110 GACGGTGGAGGGATTATCAGACCA pLX_317 10% 83% 85.3% V5 (many diffs) n/a
4 ccsbBroadEn_06708 pDONR223 100% 83% 85.3% None (many diffs) n/a
5 ccsbBroad304_06708 pLX_304 0% 83% 85.3% V5 (many diffs) n/a
6 TRCN0000478789 ATCATTCATAGAGTAAGACTGTAC pLX_317 9.9% 83% 85.3% V5 (many diffs) n/a
7 TRCN0000487742 ACACCCGCTTGAGCTACACACACC pLX_317 4.7% 83% 85.3% V5 (not translated due to prior stop codon) (many diffs) n/a
8 TRCN0000489549 GAATTTGTTGTAATTGCGTTAAAC pLX_317 18.3% 40.9% 1.1% V5 (not translated due to prior stop codon) (many diffs) n/a
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