Construct: ORF ccsbBroad304_06738
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006259.1_s304c1, BRDN0000399671
- Derived from:
- ccsbBroadEn_06738
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PLEK (5341)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_06738
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5341 | PLEK | pleckstrin | NM_002664.3 | 99.9% | 99.7% | 1019G>A |
| 2 | mouse | 56193 | Plek | pleckstrin | NM_019549.2 | 88.5% | 92% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1116
- ORF length:
- 1050
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgga accaaagcgg atcagagagg gctaccttgt gaagaagggg agcgtgttca 121 atacgtggaa acccatgtgg gttgtattgt tagaagatgg aattgaattc tataagaaga 181 aaagtgacaa cagccccaaa ggaatgatcc cgctgaaagg gagcactctg actagccctt 241 gtcaagactt tggcaaaagg atgtttgtgt ttaagatcac tacgaccaaa cagcaggacc 301 acttcttcca ggcagccttc ctggaggaga gagatgcctg ggttcgggat atcaagaagg 361 ccattaaatg cattgaagga ggccagaaat ttgccaggaa atctaccagg aggtccattc 421 gactgccaga aaccattgac ttaggtgcct tatatttgtc catgaaagac actgaaaaag 481 gaataaaaga actgaatcta gagaaggaca agaagatttt taatcactgc ttcacaggta 541 actgcgtcat tgattggctg gtatccaacc agtctgttag gaatcgccag gaaggcctca 601 tgattgcttc atcgctgctc aatgaggggt atctgcagcc tgctggagac atgtccaaga 661 gtgcagtgga tggaactgct gaaaaccctt tcctggacaa ccctgatgcc ttctactact 721 ttccagacag tgggttcttc tgtgaagaga attccagtga tgatgatgtg attctgaaag 781 aagaattcag aggggtcatt atcaagcagg gatgtttact gaagcagggg catagaagga 841 aaaactggaa agtgaggaag ttcatcttga gagaagaccc tgcctacctg cactactatg 901 accctgctgg ggcagaagat cccctgggag caattcactt gagaggctgt gtggtgactt 961 cagtggagag caactcaaat ggcaggaaga gtgaggaaga gaaccttttt gagatcatca 1021 cagcagatga agtgcactat ttcttgcaag cagccacccc caaggagcgc acagagtgga 1081 tcaaagccat ccagatggcc tcccgaactg ggaagtaccc aactttcttg tacaaagtgg 1141 ttggtaagcc tatccctaac cctctcctcg gtctcgattc tacgtagtaa tgagctagcg 1201 ctaaccggtg gcgcgttaag tcgacaatca acctctggat ta