Construct: ORF ccsbBroad304_06753
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005280.1_s304c1, BRDN0000412143
- Derived from:
- ccsbBroadEn_06753
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- POU5F1 (5460)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_06753
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6045 | RNF2 | ring finger protein 2 | NM_007212.4 | 66.7% | 49.6% | (many diffs) |
| 2 | human | 6045 | RNF2 | ring finger protein 2 | XM_011509851.3 | 66.7% | 49.6% | (many diffs) |
| 3 | human | 6045 | RNF2 | ring finger protein 2 | XM_011509852.2 | 66.7% | 49.6% | (many diffs) |
| 4 | human | 6045 | RNF2 | ring finger protein 2 | XM_005245413.3 | 63.2% | 50.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1194
- ORF length:
- 1125
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat ggcgggacac ctggcttcag attttgcctt ctcgccccct ccaggtggtg 121 gaggtgatgg gccagggggg ccggagccgg gctgggttga tcctcggacc tggctaagct 181 tccaaggccc tcctggaggg ccaggaatcg ggccgggggt tgggccaggc tctgaggtgt 241 gggggattcc cccatgcccc ccgccgtatg agttctgtgg ggggatggcg tactgtgggc 301 cccaggttgg agtggggcta gtgccccaag gcggcttgga gacctctcag cctgagggcg 361 aagcaggagt cggggtggag agcaactccg atggggcctc cccggagccc tgcaccgtca 421 cccctggtgc cgtgaagctg gagaaggaga agctggagca aaacccggag gagtcccagg 481 acatcaaagc tctgcagaaa gaactcgagc aatttgccaa gctcctgaag cagaagagga 541 tcaccctggg atatacacag gccgatgtgg ggctcaccct gggggttcta tATCAGCAAG 601 CACTCAGTCA CAGCATTGAG GAAGGACTGA AGATACAGGC CATGAACAGA CTGCAGCGAG 661 GCAAGAAACA ACAGATTGAA AATGGTAGTG GAGCAGAAGA TAATGGTGAC AGTTCACACT 721 GCAGTAATGC ATCCACACAT AGCAATCAGG AAGCAGGCCC TAGTAACAAA CGGACCAAAA 781 CATCTGATGA TTCTGGGCTA GAGCTTGATA ATAACAATGC AGCAATGGCA ATTGATCCAG 841 TAATGGATGG TGCTAGTGAA ATTGAATTAG TATTCAGGCC TCATCCCACA CTTATGGAAA 901 AAGATGACAG TGCACAGACG AGATACATAA AGACTTCTGG TAACGCCACT GTTGATCACT 961 TATCCAAGTA TCTGGCTGTG AGGTTAGCTT TAGAAGAACT TCGAAGCAAA GGTGAATCAA 1021 ACCAGATGAA CCTTGATACA GCCAGTGAGA AGCAGTATAC CATTTATATA GCAACAGCCA 1081 GTGGCCAGTT CACTGTATTA AATGGCTCTT TTTCTTTGGA ATTGGTCAGT GAGAAATACT 1141 GGAAAGTGAA CAAACCCATG GAACTTTATT ACGCACCTAC AAAGGAGCAC AAATGCCCAA 1201 CTTTCTTGTA Caaagtggtt ggtaagccta tccctaaccc tctcctcggt ctcgattcta 1261 cgtagtaatg agctagcgct aaccggtggc gcgttaagtc gacaatcaac ctctggatta