Construct: ORF ccsbBroad304_06755
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004405.1_s304c1, BRDN0000410321
- Derived from:
- ccsbBroadEn_06755
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PPAT (5471)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_06755
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5471 | PPAT | phosphoribosyl pyrophosphat... | NM_002703.5 | 99.9% | 100% | 171G>C |
| 2 | human | 5471 | PPAT | phosphoribosyl pyrophosphat... | NR_156493.2 | 39.8% | (many diffs) | |
| 3 | mouse | 231327 | Ppat | phosphoribosyl pyrophosphat... | NM_172146.2 | 85.9% | 93% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1617
- ORF length:
- 1551
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgga gctggaggag ttggggatcc gagaggaatg tggcgtgttc gggtgcatcg 121 cctcaggaga gtggcccacg cagctggatg taccgcatgt gatcactctg ggactcgtgg 181 ggctgcagca ccggggtcag gagagtgctg gtattgtgac tagtgatggg agttccgtgc 241 caacattcaa atcacacaag ggaatgggtc ttgtaaatca cgtctttact gaagacaatt 301 tgaaaaaatt atatgtttca aatcttggaa ttggacacac caggtatgcc accacaggaa 361 aatgtgaact agaaaattgt cagcccttcg ttgttgaaac acttcatggg aagatagctg 421 tggcacataa tggcgaattg gtaaatgctg ctcgattaag gaaaaagctt ctgcgtcatg 481 gtattggtct gtctacaagt tctgatagtg aaatgattac ccagttactg gcgtataccc 541 ctcctcagga acaagatgac accccagact gggtagccag gattaaaaac ttgatgaagg 601 aagcacccac agcatactcc ctgcttataa tgcacagaga tgttatttat gcagtacgag 661 atccttatgg aaatcgtccc ttatgcattg gtcgtcttat tccagtgtct gatataaatg 721 acaaagagaa aaaaacatca gaaacagaag gatgggtggt gtcttcagaa tcttgtagct 781 tcttatctat tggtgcaaga tattaccgtg aagtcttgcc tggagaaatt gtggaaatat 841 ccagacacaa tgtccaaact cttgatatta tatcaaggtc tgaaggaaac ccagtggctt 901 tttgtatctt tgaatatgtt tattttgcaa gaccagacag tatgttcgaa gaccaaatgg 961 tttatacagt aagataccgt tgtggccagc agctagcgat tgaagcacct gtggatgcag 1021 atttggttag cactgttcca gaatcTGCTA CGCCTGCTGC TCTTGCTTAC GCAGGAAAGT 1081 GTGGACTTCC ATATGTGGAG GTGCTGTGTA AAAACCGGTA TGTAGGGAGA ACCTTCATTC 1141 AGCCAAACAT GAGGTTAAGA CAACTTGGTG TTGCAAAAAA ATTTGGAGTA TTGTCAGACA 1201 ACTTTAAAGG CAAAAGAATT GTTCTTGTAG ATGATTCAAT TGTCAGAGGC AATACCATCT 1261 CACCTATAAT AAAACTGCTC AAAGAATCTG GTGCAAAAGA GGTACACATT CGAGTAGCTT 1321 CACCACCAAT TAAATATCCA TGCTTCATGG GAATAAACAT TCCTACAAAA GAAGAGCTCA 1381 TTGCCAATAA ACCAGAATTT GATCACCTTG CAGAATATCT AGGAGCAAAC AGTGTTGTGT 1441 ATCTGTCAGT AGAAGGACTG GTTTCATCTG TACAAGAAGG GATAAAGTTT AAAAAACAGA 1501 AAGAGAAAAA GCACGATATT ATGATCCAAG AAAATGGAAA TGGTCTGGAA TGTTTTGAAA 1561 AGAGTGGTCA TTGTACAGCT TGTCTCACTG GAAAATATCC TGTAGAATTA GAATGGTACC 1621 CAACTTTCTT GTACaaagtg gttggtaagc ctatccctaa ccctctcctc ggtctcgatt 1681 ctacgtagta atgagctagc gctaaccggt ggcgcgttaa gtcgacaatc aacctctgga 1741 tta